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              Open Access  
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distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research 
              
              
(Published online: 
              
              
              21-05-2015) 
              
              12.
              
              Serum 
              metabolic and minerals profile in norgestomet primed postpartum 
              anestrous surti buffaloes 
              -
              
              Sanjay 
              C. Parmar, C. T. Khasatiya, J. K. Chaudhary, R. V. Patel and H. B. 
              Dhamsaniya 
              
              Veterinary World, 8(5): 625-630   
              
   
                
                
doi: 
              10.14202/vetworld.2015.625-630   
              Sanjay C. Parmar: 
              Department of Animal Reproduction, Gynaecology and Obstetrics, 
              Anand Agricultural University, Anand, Gujarat, India; 
              
              dr.sanjayparmar@yahoo.in 
              C. T. Khasatiya: 
              Department of Veterinary Gynaecology and Obstetrics, Navsari 
              Agricultural University, Navsari, Gujarat, India; 
              
              drctkhasatiya@yahoo.com 
              J. K. Chaudhary: Sumul 
              Animal Breeding Centre, Surat, Gujarat, India; 
              
              dr.jignesh2006@gmail.com 
              R. V. Patel: Sabarmati 
              Ashram Gaushala, Bidaj Farm, Kheda, Gujarat, India; 
              
              drrohitpatel2090@gmail.com 
              H. B. Dhamsaniya: 
              Sabarmati Ashram Gaushala, Bidaj Farm, Kheda, Gujarat, India;
              
              
              drdhamsaniyahitesh@gmail.com    Received:
              27-01-2015, 
              
              Revised: 
              
              12-04-2015, Accepted:
              
              
              18-04-2015, Published 
              online: 
              
              21-05-2015   
              
              
              Corresponding author:Sanjay C. Parmar, e-mail: dr.sanjayparmar@yahoo.in 
 
              Citation: Parmar 
              SC, Khasatiya CT, Chaudhary JK, Patel RV, Dhamsaniya HB (2015) 
              Serum metabolic and minerals profile in norgestomet primed 
              postpartum anestrous surti buffaloes, Veterinary World 8(5):625-630. 
 
              Abstract 
 
              Aim: The study was 
              undertaken to find out the serum metabolic and minerals profile in 
              postpartum anestrous surti buffaloes treated with norgestomet ear 
              implants alone and in combination with pregnant mare serum 
              gonadotropin (PMSG).  
              Materials and Methods: The study was conducted on 18 
              postpartum anestrous Surti buffaloes divided into three groups of 
              six animals each at random to conduct the experiment. The 
              buffaloes in Group-I and Group-II were implanted with Crestar ear 
              implant for 9 days together with 2 ml injection of Crestar 
              solution given i/m on the day of the implant insertion. In 
              Group-II, additionally 500 IU PMSG was given i/m on the day of 
              implant removal, whereas the buffaloes in Group-III served as 
              anestrous control group and received 5 ml Normal Saline i/m on day 
              0 and 9 as a placebo treatment.  
              Results: The overall serum total protein values did not differ 
              significantly (p > 0.05) between time (days) intervals in any of 
              the groups. The mean serum total cholesterol levels at 10th
              day and on the day of estrus 
              were found significantly lower (p < 0.05) in the control group as 
              compared to treatment Groups I and II. However, there was no 
              significant difference (p > 0.05) at 10th
              day and on the day of estrus 
              between treatment groups (T1 and T2). The overall mean serum 
              cobalt, zinc, iron, and manganese values did not differ 
              significantly (p > 0.05) between different time intervals among 
              any of the groups, except copper which was significantly lower (p 
              < 0.05) at 10th 
              day in control group as compared to 
              treatment groups. 
              Conclusion: Microelements cannot be synthesized in the body. 
              Hence, it is concluded that the mineral mixture should be supplied 
              daily in the animals ration to suffice the requirement of the 
              trace elements. The mean serum metabolic and micro-minerals 
              profiles in treatment and control groups revealed that overall 
              mean serum total protein, cholesterol, copper, and zinc levels 
              were apparently higher in treatment groups whereas, mean serum 
              cobalt, iron, and manganese concentration had no consistent trend 
              between treatment and control groups of Surti buffaloes.  
              Keywords: anestrous, buffaloes, cholesterol, micro-minerals, 
              norgestomet, pregnant mare serum gonadotropin, protein.  
 
              References 
 
                
                  | 1. Acharya, R.M. (1960) Buffalo: The dominant force. Dairy 
                  India. Indian Council of Agricultural Research, New Delhi. 
                  p51-55. |  
                  |  |  
                  | 2. Valee, B.L. and Wacker, W.E.C. (1976) In: Neurath, H., 
                  editor. The Protein. Vol. 5. Academic Press, New York. |  
                  |  |  
                  | 3. Hidiroglou, M. (1979) Trace element deficiencies and 
                  fertility in ruminants: A review. J. Dairy Sci., 62(8): 
                  1195-1206. http://dx.doi.org/10.3168/jds.S0022-0302(79)83400-1
 |  
                  |  |  
                  | 4. Roberts, S.J. (1971) Veterinary Obstetrics and Genital 
                  Diseases, 2nd Edn. Scientific Book Agency, Calcutta, India. |  
                  |  |  
                  | 5. Robinson, T.J. (1977) Pregnancy. In: The Progress in 
                  Physiology of Farm Animals, 3: 793. Edr. John Hammond, Butter 
                  Worth Publication, London, United Kingdom. |  
                  |  |  
                  | 6. Krishna, G. and Ranjhan, S.K. (1980) Laboratory Manual for 
                  Nutrition Research. Vikash Publishing House Pvt. Ltd., New 
                  Delhi, India. p83-84. |  
                  |  |  
                  | 7. Steel, R.G.D. and Torrie, J.H. (1981) Principles and 
                  Procedures of Statistics, A Biometric Approach. 2nd ed. Mc 
                  Graw Hill, Int. Book Agency, Singapore. |  
                  |  |  
                  | 8. Soni, D.K. (2014) Management of Post – Partum Inactive 
                  Ovaries in Surti Buffaloes with GnRH Analogue, Toldimphos 
                  Sodium and Vitamin A, D3, E Preparation and its Effect on 
                  Biochemical Profile. M. V. Sc. Thesis Submitted to the Navsari 
                  Agricultural University, Navsari, Gujarat, India. |  
                  |  |  
                  | 9. Chaudhari, C.F. (2005) Serum biochemical and hormonal 
                  studies on induced estrus in delayed pubertal Kankrej heifers. 
                  M. V. Sc. Thesis Submitted to the Sardarkrushinagar Dantiwada 
                  Agricultural University, Sardarkrushinagar, Gujarat, India. |  
                  |  |  
                  | 10. Dhami, A.J., Khasatiya, C.T., Savaliya, F.P. and Kavani, 
                  F.S. (2006) Postpartum plasma protein profile and reproductive 
                  efficiency in anestrous and subestrous buffaloes following 
                  hormone therapy. Indian J. Field Vet., 1(3): 28-32. |  
                  |  |  
                  | 11. Khasatiya, C.T., Kharadi, V.B., Dhami, A.J., Hinsu, T.V., 
                  Panchal, M.T. and Kavani, F.S. (2005) Effect of GnRH and PGF2α 
                  treatment on conception rate and blood biochemical and mineral 
                  profile of postpartum true anestrous and subestrous Surti 
                  buffaloes. Indian J. Anim. Sci., 75(10): 1153-1158. |  
                  |  |  
                  | 12. Roberts, S.J. (1971) Veterinary Obstetrics and Genital 
                  Diseases. 2nd ed. Scientific Book Agency, Calcutta. |  
                  |  |  
                  | 13. Raju, M.S., Gupta, R.C., Khurana, N.K. and Khar, S.K. 
                  (1996) Plasma cholesterol levels in relation to superovulatory 
                  response and embryo recovery. Indian J. Anim. Reprod., 17(2): 
                  96-98. |  
                  |  |  
                  | 14. Selvaraju, S. and Rajasundaram, R.C. (2001) Effect of GnRH 
                  and eCG on serum total cholesterol (STC) profile in 
                  norgestomet primed postpartum anestrous cows. Indian Vet. J., 
                  78: 313-315. |  
                  |  |  
                  | 15. Akhtar, M.S., Farooq, A.A. and Mushtaq, M. (2010) 
                  Biochemical and hormonal profile in anestrous Nili-Ravi 
                  buffaloes. Indian Vet. J., 87: 603-604. |  
                  |  |  
                  | 16. Jayachandran, S., Nanjappan, K., Muralidharan, J., 
                  Selvaraj, P. and Manoharan. A. (2013) Blood biochemical and 
                  mineral status in cyclic and postpartum anestrous buffaloes. 
                  Int. J. Food Agric. Vet. Sci., 3(1): 99-97. |  
                  |  |  
                  | 17. Prajapati, K.R. (2011) Induction and synchronization of 
                  estrus in Surti buffaloes using controlled internal drug 
                  release (CIDR) device in different phases of breeding seasons. 
                  M. V. Sc. Thesis Submitted to be Navsari Agricultural 
                  University, Navsari, Gujarat, India. |  
                  |  |  
                  | 18. Ali, R. and Shukla, S.P. (2012) Haemato-biochemical 
                  changes in postpartum anestrous buffaloes during low breeding 
                  season. Res., 4(9): 1-2. |  
                  |  |  
                  | 19. Rushdi, M.A.E. (2010) Serum biochemical reference values 
                  for female buffaloes in Egypt. Buffalo Bull., 29(2): 141-147. |  
                  |  |  
                  | 20. Purohit, M.K. and Kohli, I.S. (1977) Variations in the 
                  blood serum cholesterol level in Rathi cow during estrus. 
                  Indian Vet. J., 54: 268-270. |  
                  |  |  
                  | 21. Hafez, E.S.E. (1980) Reproduction in Farm Animals. 4th ed. 
                  Lea and Febiger, Philadelphia. PMCid:PMC550867
 |  
                  |  |  
                  | 22. Pal, S.K., Mohanty, B.N., Ray, S.K.H. and Mohanty, S.N. 
                  (1991) Studies on serum protein, cholesterol and certain 
                  enzymes in relation to reproductive status in Bovine females. 
                  Indian J. Anim. Reprod., 12(1): 28-29. |  
                  |  |  
                  | 23. Kavani, F.S., Sharma, V.K., Siddique, G.M. and Vadodaria, 
                  V.P. (1987) Serum proteins, ascorbic acid and total 
                  cholesterol in anestrous Kankrej heifers. Indian J. Anim. 
                  Reprod., 8(2): 148-150. |  
                  |  |  
                  | 24. Shah, R.G. (1999) Hormonal and biochemical profile in 
                  fertile and infertile postpartum Surti buffaloes. Ph.D. 
                  Thesis, Gujarat Agricultural University, Anand, Gujarat, 
                  India. |  
                  |  |  
                  | 25. Underwood, E.J. (1962) Trace Elements in Human and Animal 
                  Nutrition. 2nd ed. Academic Press, Inc., New York, U.S.A. |  
                  |  |  
                  | 26. Desai, M.C., Thakkar, T.P., Amin, D.R. and Janakiraman, K. 
                  (1978) A note on serum copper levels in relation to 
                  reproductive performance in Surti buffaloes. Indian J. Anim. 
                  Sci., 48: 534-536. |  
                  |  |  
                  | 27. McDowell, L.R. (1992). Minerals in Animal and Human 
                  Nutrition. Academic Press Inc., London, United Kingdom. |  
                  |  |  
                  | 28. Wagner, W.C. (1962) Improving fertility in dairy cows. J. 
                  Am. Vet. Med. Assoc., 140: 939. PMid:14040143
 |  
                  |  |  
                  | 29. Alderman, G. (1963) Mineral nutrition in cattle. Vet. 
                  Rec., 75: 1015-1018. |  
                  |  |  
                  | 30. Pugh, D.G., Elmore, R.G. and Hembree, T.R. (1985) A review 
                  of relationship between mineral nutrition and reproduction in 
                  cattle. Bovine Pr., 20: 10. |  
                  |  |  
                  | 31. Carlson, J.C., Bhur, M.M., Wontworth, R. and Hansl, W. 
                  (1982) Evidence of membrane changes during regression in the 
                  bovine corpus luteum. Endocrinology, 110(5): 1472-1476. http://dx.doi.org/10.1210/endo-110-5-1472
 PMid:7200419
 |  
                  |  |  
                  | 32. Apagar, J. (1985) Zinc and reproduction. Anim. Nutr. Rev., 
                  5: 43. http://dx.doi.org/10.1146/annurev.nu.05.070185.000355
 PMid:3896272
 |  
                  |  |  
                  | 33. Maynard, L.A. and Loosli, J.K. (1969) Animal Nutrition. 
                  6th ed. Mc-Graw hill Company, New Delhi, India. |  
                  |  |  
                  | 34. Reddy, L.Y.S. and Reddy, S.M. (1988) Blood serum levels of 
                  iron in fertile and infertile cows. Indian J. Dairy Sci., 
                  41(1): 18-20. |  
                  |  |  
                  | 35. Chandolia, R.K. and Verma, S.K. (1987) Blood plasma trace 
                  elements in anestrous buffalo heifers. Indian J. Anim. Sci., 
                  57(3): 201-203. |  
                  |  |  
                  | 36. Kumar, A., Singh, C. and Singh, A.P. (2003) Relationship 
                  between trace mineral content of soil, fodder and animals of 
                  different age and stages of lactation. Indian J. Anim. Sci., 
                  73(4): 444-447. |  
                  |  |  
                  | 37. Das, J.M., Dutta, P., D ka, K.C., Biswas, R.K., Sarmah, 
                  B.C. and Dhali, A. (2009) Comparative study on serum macro and 
                  micro mineral profiles during estrus in repeat breeding 
                  crossbred cattle with impaired and normal ovulation. Livest. 
                  Res. Rural Dev., 21(72). Retrieved May 4, 2015, from 
                  http://www.lrrd.org/lrrd21/5/das21072.htm |  
                  |  |  
                  | 38. Noakes, D.E., Parkinson, T.J. and Gary, C.W. (2009) 
                  Veterinary Reproduction and Obstetrics. 9th ed. Saunder 
                  Elsevier, London. p833. |  
                  |  |  
                  | 39. Chaurasia, R., Kushwaha, H.S., Chaurasia, D., Gendley, 
                  M.K. and Santra, A.K. (2010) Comparative studies of certain 
                  macro minerals during various reproductive states in 
                  buffaloes. Buffalo Bull., 29(4): 291-298. |  
                  |  |  
                  | 40. El-Shahata, K.H. and Maatyb, A.M.A. (2010) The effect of 
                  dietary supplementation with calcium salts of long chain fatty 
                  acids and/ or L-carnitine on ovarian activity in Rahmani ewes. 
                  Anim. Reprod. Sci., 117(1-2): 78-82. http://dx.doi.org/10.1016/j.anireprosci.2009.04.005
 PMid:19473790
 |  
                  |  |  
                  | 41. Yusuf, M., Nakao, T., Long, S.T., Yoshida, C., Koike, K. 
                  and Hayashi, A. (2010) Reproductive performance of repeat 
                  breeders in dairy herds. Theriogenology, 73(9): 1220-1229. http://dx.doi.org/10.1016/j.theriogenology.2010.01.016
 PMid:20226515
 |  
                  |  |  
                  | 42. Akhtar, M.S., Farooq, A.A., AkbarLodhi, L., Muhammad, S., 
                  Mazhar Ayaz, M., Lashari M.H., Murtaza, S., Hussain, I., 
                  Irshad, M., Hussain, M. and Asif Raza, M. (2014) Studies on 
                  serum macro and micro minerals status in repeat breeder and 
                  normal cyclic Nili-Ravi buffaloes and their treatment 
                  strategies. Afr. J. Biotech., 13(10): 1143-1146. http://dx.doi.org/10.5897/AJB12.2328
 |  
                  |  |  
                  | 43. Ahmed, W.M., El-khadrawy, H.H., Hanafi, E.M., Ali, A.H. 
                  and Shalaby, S.A. (2010) Clinical perspective of repeat 
                  breeding syndrome in buffaloes. J. Anim. Sci., 6: 661-666. |  |