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R esearch
(Published online:
21-05-2015)
13.
Critical sources of bacterial contamination and adoption of
standard sanitary protocol during semen collection and processing
in Semen Station
-
Chandrahas Sannat, Ajit Nair, S. B. Sahu, S. A. Sahasrabudhe,
Ashish Kumar, Amit Kumar Gupta and R. K. Shende
Veterinary World, 8(5): 631-635
doi:
10.14202/vetworld.2015.631-635
Chandrahas Sannat:
Department of Veterinary Microbiology, College of Veterinary
Science & A.H., Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora,
Durg, Chhattisgarh, India;
csannat@rediffmail.com
Ajit Nair: Central
Semen Station, Livestock Development Department, Government of
Chhattisgarh, Anjora, Durg, Chhattisgarh, India;
ajitnair1971@gmail.com
S. B. Sahu: Central
Semen Station, Livestock Development Department, Government of
Chhattisgarh, Anjora, Durg, Chhattisgarh, India;
sahusb@ymail.com
S. A. Sahasrabudhe:
Central Semen Station, Livestock Development Department,
Government of Chhattisgarh, Anjora, Durg, Chhattisgarh, India;
oic2css@gmail.com
Ashish Kumar:
Department of Veterinary Microbiology, College of Veterinary
Science & A.H., Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora,
Durg, Chhattisgarh, India;
ashishvetdoc@gmail.com
Amit Kumar Gupta:
Department of Veterinary Microbiology, College of Veterinary
Science & A.H., Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora,
Durg, Chhattisgarh, India;
dramitkumaragrahari2009@gmail.com
R. K. Shende:
Department of Veterinary Microbiology, College of Veterinary
Science & A.H., Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora,
Durg, Chhattisgarh, India;
rshende786@rediffmail.com
Received:
29-12-2014,
Revised:
11-04-2015, Accepted:
19-04-2015, Published
online:
21-05-2015
Corresponding author:
Chandrahas Sannat, e-mail: csannat@rediffmail.com
Citation:
Sannat
C, Nair A, Sahu SB, Sahasrabudhe SA, Kumar A, Gupta AK, Shende RK
(2015) Critical sources of bacterial contamination and adoption of
standard sanitary protocol during semen collection and processing
in Semen Station, Veterinary World, 8(5):631-635.
Abstract
Aim: The present
investigation was conducted to locate the critical sources of
bacterial contamination and to evaluate the standard sanitation
protocol so as to improve the hygienic conditions during
collection, evaluation, and processing of bull semen in the Semen
Station.
Materials and Methods: The study compared two different
hygienic procedures during the collection, evaluation and
processing of semen in Central Semen Station, Anjora, Durg.
Routinely used materials including artificial vagina (AV) inner
liner, cone, semen collection tube, buffer, extender/diluter,
straws; and the laboratory environment like processing lab, pass
box and laminar air flow (LAF) cabinet of extender preparation
lab, processing lab, sealing filling machine, and bacteriological
lab were subjected to bacteriological examination in two phases of
study using two different sanitary protocols. Bacterial load in
above items/environment was measured using standard plate count
method and expressed as colony forming unit (CFU).
Results: Bacterial load in a laboratory environment and AV
equipments during two different sanitary protocol in present
investigation differed highly significantly (p<0.001). Potential
sources of bacterial contamination during semen collection and
processing included laboratory environment like processing lab,
pass box, and LAF cabinets; AV equipments, including AV Liner and
cone. Bacterial load was reduced highly significantly (p<0.001) in
AV liner (from 2.33±0.67 to 0.50±0.52), cone (from 4.16±1.20 to
1.91±0.55), and extender (from 1.33±0.38 to 0) after application
of improved practices of packaging, handling, and sterilization in
Phase II of study. Glasswares, buffers, and straws showed nil
bacterial contamination in both the phases of study. With slight
modification in fumigation protocol (formalin @600 ml/1000 ft3),
bacterial load was significantly decreased (p<0.001) up to 0-6 CFU
in processing lab (from 6.43±1.34 to 2.86±0.59), pass box (from
12.13±2.53 to 3.78±0.79), and nil bacterial load was reported in
LAFs.
Conclusion: Appropriate and careful management considering
critical points step by step starting right from collection of
semen to their processing can significantly minimize bacterial
contamination.
Keywords: bacterial contamination, critical sources, environment,
laboratory equipments, Semen Station.
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