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R esearch
(Published online:
22-05-2015)
16.
Morphometric evaluation of seminiferous tubule and proportionate
numerical analysis of Sertoli and spermatogenic cells indicate
differences between crossbred and purebred bulls
-
Utkarsh K. Tripathi, Shivani Chhillar, A. Kumaresan, M. K.
Muhammad Aslam, S. K. Rajak, Samiksha Nayak, A. Manimaran, T. K.
Mohanty and Savita Yadav
Veterinary World, 8(5): 645-650
doi:
10.14202/vetworld.2015.645-650
Utkarsh K. Tripathi:
Theriogenology Laboratory, Animal Reproduction, Gynaecology &
Obstetrics, National Dairy Research Institute, Karnal, Haryana,
India;
utrip09@gmail.com
Shivani Chhillar:
Theriogenology Laboratory, Animal Reproduction, Gynaecology &
Obstetrics, National Dairy Research Institute, Karnal, Haryana,
India;
shivanichhillar@gmail.com
A. Kumaresan:
Theriogenology Laboratory, Animal Reproduction, Gynaecology &
Obstetrics, National Dairy Research Institute, Karnal, Haryana,
India;
ogkumaresan@gmail.com
M. K. Muhammad Aslam:
Theriogenology Laboratory, Animal Reproduction, Gynaecology &
Obstetrics, National Dairy Research Institute, Karnal, Haryana,
India;
muhaslam@gmail.com
S. K. Rajak:
Theriogenology Laboratory, Animal Reproduction, Gynaecology &
Obstetrics, National Dairy Research Institute, Karnal, Haryana,
India;
shailendra06rajak@gmail.com
Samiksha Nayak:
Theriogenology Laboratory, Animal Reproduction, Gynaecology &
Obstetrics, National Dairy Research Institute, Karnal, Haryana,
India;
nayak.samiksha86@gmail.com
A. Manimaran:
Theriogenology Laboratory, Animal Reproduction, Gynaecology &
Obstetrics, National Dairy Research Institute, Karnal, Haryana,
India;
maranpharma@gmail.com
T. K. Mohanty:
Artificial Breeding Research Centre, National Dairy Research
Institute, Karnal, Haryana, India;
mohanty.tushar@gmail.com
Savita Yadav:
Department of Biophysics, All India Institute of Medical Sciences,
New Delhi, India;
savita11@gmail.com
Received:
16-01-2015,
Revised:
04-04-2015, Accepted:
27-04-2015, Published
online:
22-05-2015
Corresponding author:
A. Kumaresan, e-mail: ogkumaresan@gmail.com
Citation:
Tripathi UK, Chhillar S, Kumaresan A, Aslam MKM, Rajak SK, Nayak
S, Manimaran A, Mohanty TK, Yadav S (2015) Morphometric evaluation
of seminiferous tubule and proportionate numerical analysis of
Sertoli and spermatogenic cells indicate differences between
crossbred and purebred bulls, Veterinary World 8(5):645-650.
Abstract
Aim: The present study
compared the testicular cytology and histology between crossbred
(Holstein–Friesian [HF] × Tharparkar) and purebred (HF and
Tharparkar) bulls to find out differences if any.
Materials and Methods: Four peripubertal bulls from each breed
were utilized for the study. Through percutaneous needle
aspiration biopsy, Sertoli and spermatogenic cells were extracted,
and morphometry was studied. For histological studies, testicular
tissues obtained through unilateral castration were utilized.
Sertoli cells specific GATA4 antibody was used to study the
population of Sertoli cells in the seminiferous tubule through
immunofluorescence.
Results: The testicular weight, volume, and scrotal
circumference differed significantly among the breeds. The
diameter and area of the seminiferous tubule was high in HF,
followed by Karan Fries (KF), and Tharparkar bulls. However, the
degree of compactness, based on qualitative evaluation, was high
in Tharparkar followed by KF and HF bulls. The intensity of Leydig
cells was higher in Tharparkar bulls followed by KF and HF. The
proportion of Sertoli cells was higher (p<0.05) in HF and
Tharparkar bulls compared to KF bulls.
Conclusion: It may be concluded that variations exist in
testicular components of the breeds studied and the proportion of
Sertoli cells in relation to spermatogenic cells was significantly
lower in crossbred bulls compared to purebred bulls.
Keywords: crossbred bulls, purebred bulls, seminiferous
tubules, Sertoli cells, testicular histology.
References
1. Mukhopadhyay, C.S., Gupta, A.K., Yadav, B.R., Khate, K.,
Raina, V.S., Mohanty, T.K. and Dubey, P.P. (2010) Subfertility
in males: An important cause of bull disposal in bovines.
Asian Aust. J. Anim. Sci., 23: 450-455.
http://dx.doi.org/10.5713/ajas.2010.90298 |
|
|
|
2. Vijetha, B.T., Rajak, S.K., Layek, S.S., Kumaresan, A.,
Mohanty, T.K., Chakravarty, A.K., Gupta, A.K., Muhammad Aslam,
M.K., Manimaran, A. and Prasad, S. (2014) Breeding soundness
evaluation in crossbred bulls: Can testicular measurements be
used as a tool to predict ejaculate quality? Indian J. Anim.
Sci., 84(2): 177-180. |
|
|
|
3. Mandal, D.K. and Tyagi, S. (2004) Pre-copulatory behaviour
of Sahiwal bulls during semen collection and effects of age
and season on their sexual performance. Indian J. Dairy Sci.,
57: 334-338. |
|
|
4. Khatun, M., Kaur, S., Kanchan and Mukhopadhyay, C.S. (2013)
Subfertility problems leading to disposal of breeding bulls.
Asian-Aust. J. Anim. Sci., 26(3): 303-308.
http://dx.doi.org/10.5713/ajas.2012.12413
PMid:25049791 PMCid:PMC4093486 |
|
|
|
5. Rajak, S.K., Vijetha, B.T., Kumaresan, A., Lyek, S.S.,
Mohanty, T.K., Gaurav, M.K., Chakravarty, A.K., Dutta, T.K.,
Manimaran, A. and Prasad, S. (2014) Testicular cytology
indicate differences in Sertoli cell counts between "good
freezer" and "poor freezer" bulls. Indian J. Exp. Biol. [In
press] |
|
|
6. Aslam, M.K.M., Kumaresan, A., Sharma, V.K., Tajmul, M.,
Chhillar, S., Chakravarty, A.K., Manimaran, A., Mohanty, T.K.,
Srinivasan, A. and Yadav, S. (2014) Identification of putative
fertility markers in seminal plasma of crossbred bulls through
differential proteomics. Theriogenology, 82(9): 1254-1262.
http://dx.doi.org/10.1016/j.theriogenology.2014.08.007
PMid:25258256 |
|
|
7. Johnson, L. (1991) Spermatogenesis. In: Cupps, P.T.,
editors. Reproduction in Domestic Animals. Academic Press
Inc., San Diego, California. p173-219.
http://dx.doi.org/10.1016/b978-0-08-057109-6.50010-0 |
|
|
|
8. Franca, L.R. and Russel, L.D. (1998) The testis of domestic
animals. In: Martinez, F. and Regadera, J., editors. Male
Reproduction: A Multidisciplinary Overview. Churchill
Livingstone, Madrid, Spain. p197-219. |
|
|
9. Franca, L.R. and Godinho, C.L. (2003) Testis morphometry,
seminiferous epithelium cycle length, and daily sperm
production in domestic cats (Felis catus). Biol. Reprod.,
68(5): 1554-1561.
http://dx.doi.org/10.1095/biolreprod.102.010652
PMid:12606460 |
|
|
10. Tripathi, U.K., Aslam, M.K.M., Pandey, S., Nayak, S.,
Chhillar, S., Srinivasan, A., Mohanty, T.K., Kadam, P.H.,
Chauhan, M.S. Yadav, S. and Kumaresan, A. (2014) Differential
proteomic profile of spermatogenic and Sertoli cells from
peri-pubertal testes of three different bovine breeds. Front
Cell Dev. Biol., 2: 24.
http://dx.doi.org/10.3389/fcell.2014.00024
PMid:25364731 PMCid:PMC4206989 |
|
|
|
11. Rajak, S.K., Kumaresan, A., Gaurav, M.K., Aslam, M.K.M.,
Mohanty, T.K., Prasad, S., Chakravarty A.K. and
Venkatasubramanian, V. (2013) Testicular biometry and semen
quality is not altered by the process of fine needle
aspiration in crossbred bulls. Indian J. Anim. Sci., 83(7):
732-735. |
|
|
12. Leme, D.P. and Papa, F.O. (2010) Cytological
identification and quantification of testicular cell types
using fine needle aspiration in horses. Equine Vet. J., 32:
444-446.
http://dx.doi.org/10.2746/042516400777591156 |
|
|
13. Santos, M., Marcos, R. and Caniatti, M. (2010) Cytologic
study of normal canine testis. Theriogenology, 73(2): 208-214.
http://dx.doi.org/10.1016/j.theriogenology.2009.07.030
PMid:19906415 |
|
|
14. Baker, J.R. (1945) Cytological Technique. 2nd ed. Methuen,
London, UK.
PMCid:PMC1934164 |
|
|
15. Tarulli, G.A., Stanton, P.G., Loveland, K.L., Meyts, E.R.,
McLachlan, R.I. and Meachem, S.J. (2013) A survey of Sertoli
cell differentiation in men after gonadotropin suppression and
in testicular cancer. Spermatogenesis, 3(1): e24014.
http://dx.doi.org/10.4161/spmg.24014 |
|
|
|
16. Sudheer, S. and Xavier, C.J. (2000) Disposal pattern of
breeding bulls in Kerala. Indian J. Anim. Reprod., 21(1):
72-73. |
|
|
17. Arrighi, S., Bosi, G., Gropeptti, D. and Cremonesi, F.
(2010) Morpho – and histometric evaluation on the testis and
epididymis in buffalo bulls during different reproductive
seasons. TOANATJ., 2: 29-33.
http://dx.doi.org/10.2174/1877609401002010029 |
|
|
|
18. Al-Sahaf, M.M.H. and Ibrahim, N.S. (2012) The effect of
months on physical of sperms and morphological alterations in
the testes and epididymis of Iraqi buffalo. Iraqi J. Vet.
Med., 36: 204-220. |
|
|
19. Brito, L.F.C., Barth, A.D., Wilde, R.E. and Kastelic, J.P.
(2012) Effect of growth rate from 6 to 16 mo of age on sexual
development and reproductive function in beef bulls.
Theriogenology, 77(7): 1398-1405.
http://dx.doi.org/10.1016/j.theriogenology.2011.11.003
PMid:22225693 |
|
|
|
20. Sudheer, S. (2000) Relationship of age and scrotal
circumference with the seminal characteristics of crossbred
bulls. Indian J. Anim. Sci., 70(8): 804-806. |
|
|
21. Rajak, S.K., Kumaresan, A., Gaurav, M.K., Layek, S.S.,
Mohanty, T.K., Muhammad Aslam, M.K., Tripathi, U.K., Prasad,
S. and De, S. (2014) Testicular cell indices and peripheral
blood testosterone concentrations in relation to age and semen
quality in crossbred (Holstein Friesian x Tharparkar) bulls.
Asian-Aust. J. Anim. Sci., 27(11): 1554-1561.
http://dx.doi.org/10.5713/ajas.2014.14139
PMid:25358314 PMCid:PMC4213699 |
|
|
22. Berndtson, W.E., Igboeli, G. and Parker, W.G. (1987a) The
numbers of Sertoli cells in mature Holstein bulls and their
relationship to quantitative aspects of spermatogenesis. Biol.
Reprod., 37(1): 60-67.
http://dx.doi.org/10.1095/biolreprod37.1.60
PMid:3651551 |
|
|
23. Blanchard, T.L. and Johnson, L. (1997) Determination of
daily sperm production in stallion testes by enumeration of
germ cells in homogenates. Theriogenology, 47: 655-664.
http://dx.doi.org/10.1016/S0093-691X(97)00024-1 |
|
|
24. Griswold, M.D. (1998) The central role of Sertoli cells in
spermatogenesis. Semin. Cell Dev. Biol., 9(4): 411-416.
http://dx.doi.org/10.1006/scdb.1998.0203
PMid:9813187 |
|
|
|
25. Russell, L.D. and Griswold, M.D. (1993) The Sertoli Cell.
Cache River Press, Clearwater, Florida. |
|
|
26. Berndtson, W.E., Igboeli, G., Pickett, B.W. (1987b)
Relationship of absolute numbers of Sertoli cells to
testicular size and spermatogenesis in young beef bulls. J.
Anim. Sci., 64(1): 241-246.
PMid:3818487 |
|
