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Research (Published online: 22-05-2015)

16. Morphometric evaluation of seminiferous tubule and proportionate numerical analysis of Sertoli and spermatogenic cells indicate differences between crossbred and purebred bulls - Utkarsh K. Tripathi, Shivani Chhillar, A. Kumaresan, M. K. Muhammad Aslam, S. K. Rajak, Samiksha Nayak, A. Manimaran, T. K. Mohanty and Savita Yadav

Veterinary World, 8(5): 645-650

 

 

   doi: 10.14202/vetworld.2015.645-650

 

Utkarsh K. Tripathi: Theriogenology Laboratory, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana, India; utrip09@gmail.com

Shivani Chhillar: Theriogenology Laboratory, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana, India; shivanichhillar@gmail.com

A. Kumaresan: Theriogenology Laboratory, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana, India; ogkumaresan@gmail.com

M. K. Muhammad Aslam: Theriogenology Laboratory, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana, India; muhaslam@gmail.com

S. K. Rajak: Theriogenology Laboratory, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana, India; shailendra06rajak@gmail.com

Samiksha Nayak: Theriogenology Laboratory, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana, India; nayak.samiksha86@gmail.com

A. Manimaran: Theriogenology Laboratory, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana, India; maranpharma@gmail.com

T. K. Mohanty: Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, Haryana, India; mohanty.tushar@gmail.com

Savita Yadav: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India; savita11@gmail.com

 

Received: 16-01-2015, Revised: 04-04-2015, Accepted: 27-04-2015, Published online: 22-05-2015

 

Corresponding author: A. Kumaresan, e-mail: ogkumaresan@gmail.com


Citation: Tripathi UK, Chhillar S, Kumaresan A, Aslam MKM, Rajak SK, Nayak S, Manimaran A, Mohanty TK, Yadav S (2015) Morphometric evaluation of seminiferous tubule and proportionate numerical analysis of Sertoli and spermatogenic cells indicate differences between crossbred and purebred bulls, Veterinary World 8(5):645-650.



Aim: The present study compared the testicular cytology and histology between crossbred (Holstein–Friesian [HF] Tharparkar) and purebred (HF and Tharparkar) bulls to find out differences if any.

Materials and Methods: Four peripubertal bulls from each breed were utilized for the study. Through percutaneous needle aspiration biopsy, Sertoli and spermatogenic cells were extracted, and morphometry was studied. For histological studies, testicular tissues obtained through unilateral castration were utilized. Sertoli cells specific GATA4 antibody was used to study the population of Sertoli cells in the seminiferous tubule through immunofluorescence.

Results: The testicular weight, volume, and scrotal circumference differed significantly among the breeds. The diameter and area of the seminiferous tubule was high in HF, followed by Karan Fries (KF), and Tharparkar bulls. However, the degree of compactness, based on qualitative evaluation, was high in Tharparkar followed by KF and HF bulls. The intensity of Leydig cells was higher in Tharparkar bulls followed by KF and HF. The proportion of Sertoli cells was higher (p<0.05) in HF and Tharparkar bulls compared to KF bulls.

Conclusion: It may be concluded that variations exist in testicular components of the breeds studied and the proportion of Sertoli cells in relation to spermatogenic cells was significantly lower in crossbred bulls compared to purebred bulls.

Keywords: crossbred bulls, purebred bulls, seminiferous tubules, Sertoli cells, testicular histology.



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