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R esearch
(Published online:
26-05-2015)
18.
Pharmacodynamic interaction of fenugreek,
insulin and glimepiride on sero-biochemical parameters in diabetic
Sprague-Dawley rats - C. Haritha, A. Gopala Reddy, Y.
Ramana Reddy and B. Anilkumar
Veterinary World, 8(5): 656-663
doi:
10.14202/vetworld.2015.656-663
C.
Haritha:
Department of Pharmacology, College of Veterinary Science, Sri
Venkateswara Veterinary University, Korutla, Telangana, India;
dr.haritha03@gmail.com
A.
Gopala Reddy:
Department of Pharmacology, College of Veterinary Science, Sri
Venkateswara Veterinary University, Rajendranagar, Hyderabad,
Telangana, India;
gopalareddy123@gmail.com
Y.
Ramana Reddy:
Department of Animal Nurtition, College of Veterinary Science, Sri
Venkateswara Veterinary University, Rajendranagar, Hyderabad,
Telangana, India; ramanayr19@yahoo.co.in
B.
Anilkumar: Department of Pharmacology, College of Veterinary
Science, Sri Venkateswara Veterinary University, Korutla,
Telangana, India;
anilvetpharma@gmail.com
Received: 04-01-2015, Revised: 12-04-2015, Accepted: 21-04-2015,
Published online: 26-05-2015
Corresponding author:
B. Anilkumar, e-mail: anilvetpharma@gmail.com
Citation:
Haritha C, Gopala
Reddy A, Ramana Reddy Y, Anilkumar B (2015) Pharmacodynamic
interaction of fenugreek, insulin and glimepiride on sero-biochemical
parameters in diabetic Sprague-Dawley rats, Veterinary World 8(5):656-663.
Abstract
Aim:
This study was undertaken to assess the pharmacodynamic
interaction of fenugreek, insulin and glimepiride on
serobiochemical parameters in streptozotocin-induced diabetic
rats.
Materials and Methods: A total of 56 male Sprague-Dawley rats,
randomly divided into seven Groups. Group 1: Non-diabetic control;
Group 2: Streptozotocin induced diabetic control; Groups 3, 4 and
5 were treated with insulin, glimepiride and fenugreek seed
powder, respectively; Groups 6 and 7: Insulin + fenugreek seed
powder treatment and glimepiride + fenugreek seed powder treatment
respectively, in diabetic rats. Body weights, blood glucose,
lipids total cholesterol (TC), high-density lipoprotein (HDL),
low-density lipoprotein (LDL), triglycerides (TG) and proteins
(total protein, albumin, globulin, A/G ratios) were studied at
different time intervals. Rats were sacrified at the end of 8
weeks, pancreas and aorta collected for histopathological study.
Results: The results of Group 2 showed significantly (p<0.05)
higher concentration of glucose, TC, TG, LDL, globulin, A/G ratios
and significantly (p<0.05) lower concentration of albumin, total
protein, HDL and body weights when compared to Group 1 at the end
of 4 th and 8th weeks
intervals with marked alteration in histopathology of pancreas and
aorta. All the treatment Groups 3-7 showed significantly (p<0.05)
improvement in the all the parameters and the Groups 6 and 7
showed highest decrease in the concentration blood glucose, TC,
TG, LDL and increase in the albumin, total protein and body
weights during 6th and 8th
week, respectively.
Conclusion: The treatment with fenugreek, insulin and
glimepiride countered the alteration in the sero biochemical
parameters in diabetic rats, and their combination was found a
positive interaction in improving the sero biochemical status of
diabetic rats.
Keywords: cholesterol, diabetes, fenugreek,
glimepiride, glucose, insulin, protein.
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