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Research (Published online: 06-05-2015)

5. Acute and subchronic toxicity assessment model of Ferula assa-foetida gum in rodents - Ayman Goudah, Khaled Abo-El-Sooud and Manal A. Yousef

Veterinary World, 8(5): 584-589



   doi: 10.14202/vetworld.2015.584-589


Ayman Goudah: Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;

Khaled Abdo-El-Sooud: Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;

Manal A. Yousef: Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;


Received: 11-12-2014, Revised: 01-04-2015, Accepted: 10-04-2015, Published online: 06-05-2015


Corresponding author: Ayman Goudah, e-mail address:

Citation: Goudah A, Abo-EL-Sooud K, Yousef MA (2015) Acute and subchronic toxicity assessment model of Ferula assa-foetida gum in rodents, Veterinary World 8(5);584-589.

Aim: The present study was performed to investigate acute and subchronic oral toxicity of Ferula assa-foetida gum (28 days) in Sprague Dawley rats.

Materials and Methods: Acute oral administration of F. assa-foetida was done as a single bolus dose up to 5 g/kg in mice and subchronic toxicity study for 28 days was done by oral administration at doses of 0 (control) and 250 mg/kg in Sprague Dawley rats.

Results: The obtained data revealed that oral administration of F. assa-foetida extract in rats for 28 successive days had no significant changes on body weight, body weight gain, the hematological parameters in rats all over the period of the experiment, and there are no significant increases in the activity of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine and urea. Liver of treated rats showed mild changes as thrombosis and sinusoidal leukocytosis. It also showed portal infiltration with inflammatory cells, while kidney of treated rat showed an atrophy of glomerular tuft, thickening of parietal layer of Bowman capsule, and focal tubular necrosis. It also showed dilatation and congestion of renal blood vessels.

Conclusion: We concluded that F. assa-foetida gum had broad safety and little toxicity for short term use in dose of 250 mg/kg.

Keywords: F. assa-foetida, histopathology, rats, serum enzymatic activity, subchronic toxicity.

1. Ateba, S.B., Simo, R.V., Mbanya, J.C., Krenn, L. and Njamen, D. (2014) Safety profile and gender specific differences of a methanol extract of Eriosema laurentii (Leguminosae) in acute and subchronic (28 days) oral toxicity studies in Wistar rats. Food Chem. Toxicol., 65:27-32.
2. Mahendra, P. and Bisht, S. (2012) Ferula asafoetida: Traditional uses and pharmacological activity. Pharmacogn. Rev., 6(12):141-146.
PMid:23055640 PMCid:PMC3459456
3. Fabricant, D.S. and Farnsworth, N.R. (2001) The value of plants used in traditional medicine for drug discovery. Environ. Health Persp.,109 Suppl 1:69-75.
PMid:11250806 PMCid:PMC1240543
4. Zhang, Y. Kim, K.H. Zhang, W. Guo, Y. Kim, S.H. and Lü, J. (2012) Galbanic acid decreases androgen receptor abundance and signaling and induces G1 arrest in prostate cancer cells. Int. J. Cancer, 130(1):200-212.
PMid:21328348 PMCid:PMC3137900
5. Lee, C.L. Chiang, L.C. Cheng, L.H. Liaw, C.C. Abd El-Razek, M.H. Chang, F.R. and Wu, Y.C. (2009) Influenza A (H1N1) Antiviral and Cytotoxic Agents from Ferula assafoetida. J. Nat. Prod., 72(9):1568-1572.
6. Nabavi, S.M. Ebrahimzadeh, M.A. Nabavi, S.F. Eslami, B. and Dehpour, A.A. (2011) Antioxidant and antihaemolytic activities of Ferula foetida regel (Umbelliferae). Eur. Rev. Med. Pharmaco., 15(2):157-164.
7. Angelini, P. Pagiotti, R. Venanzoni, R. and Granetti, B. (2009) Antifungal and allelopathic effects of asafoetida against Trichoderma harzianum and Pleurotus spp. Allelopathy J., 23(1):357-368.
8. Abu-Zaiton, A.S. (2010) Anti-diabetic activity of Ferula assafoetida extract in normal and alloxan-induced diabetic rats. Pak. J. Biol. Sci., 13(2):97-100.
9. Kumar, P. and Singh, D.K. (2006) Molluscicidal activity of Ferula asafoetida, Syzygium aromaticum and Carum carvi and their active components against the snail Lymnaea acuminata. Chemosphere, 63(9):1568-1574.
10. Fatehi, M. Farifteh, F. and Fatehi-Hassanabad, Z. (2004) Antispasmodic and hypotensive effects of Ferula asafoetida gum extract. J. Ethnopharmacol., 91(2-3):321-324.
11. Abd El-Razek, M.H. Ohta, S. Ahmed, A.A. and Hirata, T. (2001) Sesquiterpene coumarins from the roots of Ferula assafoetida. Phytochemistry, 58(8):1289-1295.
12. Lorke, D. (1983) A new approach to practical acute toxicity testing. Arch. Toxicol., 54(4):275-287.
13. Pershin, G.K. (1971) Methods of experimental chemotherapy: Practical guidance. Second edition, ed. Second edition, Medicina, Moscow.
14. Merghani, T.H. (2010) The Core of Medical Physiology. 3rd ed.,pp. 157-158.
15. Reitman, S. and Frankel, S. (1957): A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol., 28(1):56-63.
16. Kroll, M.H. Roach, N.A. Poe, B. and Elin, R.J. (1987): Mechanism of interference with the Jaffe reaction for creatinine. Clin. Chem., 33(7):1129-1132.
17. Kind, P.R. and King, E.J. (1954) Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J. Clin. Pathol., 7(4):322-326.
18. Wills, M.R. and Savory, J. (1981) Biochemistry of renal failure. Ann. Clin. Lab. Sci., 11(4):292-299.
19. Mansurov, M.M. and Martirosov, M.S. (1990) The action of the sodium salt of galbanic acid in experimental erythrocyte hyperaggregation. Farmakol. Toksikol., 53(1):51-53.
20. Kim, K.H. Lee, H.J. Jeong, S.J. Lee, H.J. Lee, E.O. Kim, H.S. Zhang, Y. Ryu, S.Y. Lee, M.H. Lu, J. and Kim, S.H. (2011) Galbanic acid isolated from Ferula assafoetida exerts in vivo anti-tumor activity in association with anti-angiogenesis and anti-proliferation. Pharmaceut. Res., 28 (3): 597-609.
21. Jiang, C. Lee, H.J. Li, G.X. Guo, J. Malewicz, B. Zhao, Y. Lee, E.O. Lee, H.J. Lee, J.H. Kim, M.S. Kim, S.H. and Lu, J. (2006) Potent antiandrogen and androgen receptor activities of an Angelica gigas-containing herbal formulation: identification of decursin as a novel and active compound with implications for prevention and treatment of prostate cancer. Cancer Res., 66 (1): 453-463.
22. Guo, J. Jiang, C. Wang, Z. Lee, H.J. Hu, H. Malewicz, B. Lee, H.J. Lee, J.H. Baek, N.I. Jeong, J.H. Kim, D.K. Kang, K.S. Kim, S.H. and Lu, J. (2007) A novel class of pyranocoumarin anti-androgen receptor signaling compounds. Mol. Cancer. Ther., 6(3): 907-917.
23. Rajanikanth, B. Ravindranath, B. and Shankaranarayana, M.L. (1984) Volatile polysulphides of asafoetida. Phytochemistry, 23(4):899-900.
24. Hofer, O. Widhalm, M. and Greper, H. (1984) Circular dichroism of sesquitergeneumbelliferone ethers and structure elucidation of a new derivative isolated from the gum resin 'assafoetida. Monatsh. Chem., 115:1207-1218.
25. Iranshahi, M. (2012) A review of volatile sulfur-containing compounds from terrestrial plants: biosynthesis, distribution and analytical methods. J. Essent. Oil Res., 24(4):393-434.
26. Harper, H.A. (1973) Review of physiological chemistry. Los Altos, California.: 14th ed. Lange Medical Publications.
27. David, K. Weber, K. Danielson, S. Wright, J. and Foley, E. (2002) Hematology and serum biochemistry values of rats. J. Wildlife Dis., 38(3):576-582.
29. Cotran, R. Kumar, V. and Robins, S. (1989) Robin's pathological basis of disease. 4th edn. W.B Saunders Co. Harcourt. Pp: 212-217.
30. Adesokan, A.A. and Akanji, M.A. (2004) Effect of administration of aqueous extract of Enantia chlorantha on the activities of some enzymes in the small intestine of rats. Niger. J. Biochem. Mol. Biol., 18:103-105.
31. Ramaiah, S.K. (2007) A toxicologist guide to the diagnostic interpretation of hepatic biochemical parameters. Food and chemical toxicology. Food Chem. Toxicol., 45(9):1551-1557.
32. Han, Y.D. Song, S.Y. Lee, J.H. Lee, D.S. and Yoon, H.C. (2011) Multienzyme-modified biosensing surface for the electrochemical analysis of aspartate transaminase and alanine transaminase in human plasma. Anal. Bioanal. Chem., 400(3):797-805.
33. Ozer, J. Ratner, M. Shaw, M. Bailey, W. and Schomaker, S. (2008): The current state of serum biomarkers of hepatotoxicity. Toxicology, 245(3):194-205.
34. Lameire, N. Van Biesen, W. and Vanholder, R. (2005) Acute renal failure. Lancet, 365(9457):417-430.
35. Kelly, K.J. Neu, J. Camitta, B.M. and Honig, G.R. (1984) Methemoglobinemia in an infant treated with the folk remedy glycerited asafoetida. Pediatrics, 73(5):717-719.
36. Emami, A. Fasihi, S. and Mehregan, I. (2010) Medicinal Plants. Andisheh Avar, Tehran.