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R esearch
(Published online:
14-05-2015)
7.
Detection and characterization of
extended-spectrum β-lactamases (blaCTX-M-1
and
blaSHV)
producing Escherichia coli, Salmonella spp.
and
Klebsiella pneumoniae isolated from humans in Mizoram -
Iadarilin Warjri, T. K. Dutta, H. Lalzampuia and Rajesh Chandra
Veterinary World, 8(5): 599-604
doi:
10.14202/vetworld.2015.599-604
Iadarilin Warjri:
Department of Veterinary Microbiology, Central Agricultural
University, Selesih, Aizawl, Mizoram, India;
iadawarjri@gmail.com
T. K.
Dutta:
Department of Veterinary Microbiology, Central Agricultural
University, Selesih, Aizawl, Mizoram, India;
tapandutta@rediffmail.com
H.
Lalzampuia:
Department of Veterinary Microbiology, Central Agricultural
University, Selesih, Aizawl, Mizoram, India;
ateahlawndo@gmail.com
Rajesh
Chandra: Department of Veterinary Microbiology, Central
Agricultural University, Selesih, Aizawl, Mizoram, India;
rajeshchandra2k@rediffmail.com
Received:
18-12-2014, Revised: 05-04-2015, Accepted: 11-04-2015, Published
online: 14-05-2015
Corresponding author:
T. K. Dutta, e-mail: tapandutta@rediffmail.com
Citation:
Warjri I, Dutta TK,
Lalzampuia H, Chandra R (2015) Detection and characterization of
extended-spectrum β-lactamases (blaCTX-M-1 and blaSHV)
producing Escherichia coli, Salmonella spp.
and Klebsiella pneumoniae isolated from humans in Mizoram,
Veterinary World 8(5):599-604.
Abstract
Aim:
The present study was
conducted to isolate and characterize the extended spectrum β-lactamases
(ESBLs) producing enteric bacteria in human beings in Mizoram,
India.
Materials and Methods:
Fecal samples were collected from human
beings with or without the history of diarrhea from different
hospitals of Mizoram. Samples were processed for isolation and
identification of Escherichia coli,
Salmonella and
Klebsiella pneumoniae.
All the isolates were subjected to antibiotic sensitivity assays.
Phenotypically, ESBLs production ability was determined by double
discs synergy test (DDST) method. ESBLs producing isolates were
subjected to polymerase chain reaction (PCR) for detection of
ESBLs genes. Plasmids were cured by acridine orange. Transfer of
resistance from a donor to recipient strains was done by
in vitro
horizontal method.
Results: A total of 414
enteric bacteria were isolated from 180 fecal samples (113 were
from diarrheic patients and 67 were from non-diarrheic patients),
of which 333 (80.44%), 52 (12.56%), and 29 (7.00%) were
E. coli,
K. pneumoniae
and Salmonella
spp., respectively. Double
discs synergy test (DDST) exhibited 72 (21.62%)
E. coli, 12
(23.08%) K. pneumoniae
and 4 (13.79%)
Salmonella spp. were ESBLs
producers. Altogether, 24 (13.04%) isolates were found to be
positive for at least one resistance genes under this study. A
total of 36 (8.70%) E. coli,
4 (0.97%) K. pneumoniae
and 2 (0.48%)
Salmonella spp. were found to
be positive for blaCTX-M-1
gene by PCR. Similarly, 5 (1.21%)
E. coli and
4 (0.97%) K. pneumoniae
isolates were found to be positive for
blaSHV
gene. A total of 3 (0.72%)
K. pneumoniae
isolates were recorded as positive for
both blaCTX-M-1
and blaSHV
genes. All the isolates were carrying
plasmids ranging between 0.9 kb and ~30 kb. The resistance plasmid
could not be transferred to a recipient by
in vitro horizontal gene
transfer method.
Conclusion: ESBLs producing
enteric bacteria are circulating in human population in North
Eastern Region of India. Indiscriminate use of antibiotics should
be avoided to control the menace of multidrug resistance bacteria
in the environment, animals, and human beings.
Keywords: Enterobacteriaceae,
extended spectrum β-lactamases, India,
Mizoram.
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