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Research (Published online: 24-11-2015)

12. Biochemical studies in experimentally Escherichia coli infected broiler chicken supplemented with neem (Azadirachta indica) leaf extract - Vikash Sharma, K. K. Jakhar, Vikas Nehra and Sarvan Kumar

Veterinary World, 8(11): 1340-1345

 

 

   doi: 10.14202/vetworld.2015.1340-1345

 

Vikash Sharma: Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, Haryana, India; sharmavikashjind@gmail.com

K. K. Jakhar: Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, Haryana, India; hod.vpp@luvas.edu.in

Vikas Nehra: Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, Haryana, India; drvikasnehra@gmail.com

Sarvan Kumar: Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, Haryana, India; drsrvn38@gmail.com

 

Received: 24-06-2015, Revised: 10-10-2015, Accepted: 17-10-2015, Published online: 24-11-2015


Corresponding author:
Vikash Sharma, e-mail: sharmavikashjind@gmail.com


Citation: Sharma V, Jakhar KK, Nehra V, Kumar S (2015) Biochemical studies in experimentally Escherichia coli infected broiler chicken supplemented with neem (Azadira chta indica) leaf extract, Veterinary World 8(11): 1340-1345.



Aim: An experimental study was conducted on 192-day-old broiler chicks for evaluating the effect of 10% neem leaf extract (NLE) supplementationon biochemical parameters in chickens experimentally infected with Escherichia coli O78 at 107 CFU/0.5 ml at 7 days of age.

Materials and Methods: The 192-day-old broiler chicks were procured. These chicks were divided into two groups (A and B) containing 96 birds each on the 1st day. Diet of all the chicks of Group A was supplemented with 10%NLE in water, whereas chicks of Group B were given feed and water devoid of NLE supplementation throughout the experiment. After rearing for 1 week, chicks of both the groups (A and B) were again divided into two subgroups (Group A into A1 and A2 and Group B into B1 and B2) of 54 and 42 birds, respectively. At the age of 7 days all the chicks of groups A1 and B1 were injected with E. coli O78 at 107 CFU/0.5 ml intraperitoneally. Blood samples were collected from six chicks from each group at day 0, 2, 4, 7, 14, 21, 28 days post-infection and serum was separated for biochemical studies.

Results: There was a significant increase in serum alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) activities, globulin concentration and a decrease in total protein (TP), albumin concentrations, and alkaline phosphatase (ALP) activity in both the infected groups. However, the changes in biochemical values, i.e., ALT, AST, LDH, ALP, TP, albumin, and globulin wereof lower magnitude in NLE supplemented group suggesting hepatoprotective and cardioprotective effect of NLE.

Conclusions: Fromthe present study, it is reasonable to conclude that significant increase in the value of ALT, AST, LDH, globulin, and significant decrease in the value of ALP, TP, and albumin was of lower magnitude in supplemented infected group (A1) as compared to non-supplemented infected group (B1) suggesting hepatoprotective and cardioprotective effect of NLE.

Keywords: biochemical studies, chicken, experimentally Escherichia coli infected broiler, neem leaf extract.



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