Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
R esearch
(Published online:
24-11-2015)
13.
Clinicopathological evaluation of
non-parasitic dermatoses in canines -
M. J. Sindha, B. J. Trangadia, P. D. Vihol, R. S. Parmar and B.
V. Patel
Veterinary World, 8(11): 1346-1350
doi:
10.14202/vetworld.2015.1346-1350
M. J. Sindha:
Animal Disease Diagnostic Laboratory, Amul Dairy, Mogar,
Gujarat, India;
drmehulpathologist@gmail.com
B. J. Trangadia:
Department of Veterinary Pathology, College of Veterinary
Science & Animal Husbandry, Navsari Agricultural University,
Navasari, Gujarat, India;
drbjt77@gmail.com
P. D. Vihol:
Department of Veterinary Pathology, College of Veterinary
Science & Animal Husbandry, Navsari Agricultural University,
Navasari, Gujarat, India;
drpritivet@gmail.com
R. S. Parmar:
Poultry Complex, College of Veterinary Science & Animal
Husbandry, Anand Agricultural University, Anand, Gujarat, India;
drrohit.vet23@rediffmail.com
B. V. Patel:
Cambay Satellite Dairy, Amul Dairy, Undel, Gujarat, India;
bhavesh4yuva@gmail.com
Received: 15-07-2015, Revised: 11-10-2015, Accepted: 19-10-2015,
Published online: 24-11-2015
Corresponding author:
M. J. Sindha, e-mail: drmehulpathologist@gmail.com
Citation:
Sindha MJ, Trangadia BJ, Vihol PD, Parmar RS, Patel BV
(2015) Clinicopathological evaluation of non-parasitic
dermatoses in canines,
Veterinary World 8(11):
1346-1350.
Abstract
Aim:
The present study has been carried out to detect non-parasitic
dermatoses in canines brought at the Nandini Veterinary
Hospital, Surat.
Materials and Methods: The current investigation was
carried out on skin scrapping, skin biopsy specimens, blood, and
serum samples of 210 freshly registered cases of dogs with
dermatological afflictions. Dogs found healthy on clinical
examination were used as control animals (n=15). The incidence
of non-parasitic dermatoses has been recorded as per age, breed,
and sex of dogs. For bacterial isolation, the pus/exudates
samples were collected from 40 cases of pyoderma and streaked
onto brain-heart infusion agar while 13 skin scrapping samples
were inoculated on Sabouraud’s dextrose agar with
chloramphenicol for isolation of fungi. The organisms were
identified on the basis of gross and microscopic observation of
cultural growth on media. The blood and sera samples were also
collected to note alteration in hematology and biochemical
parameters, respectively. Tissue samples from lesions were
collected and subsequently preserved in 10% neutral buffered
formalin for histopathology.
Results: Out of 210 cases of dermatoses, 60 cases were of
non-parasitic dermatoses, i.e., 28.57%. Of these, bacterial skin
infections (pyoderma) were found to be the predominant at
80.00%, followed by other non-parasitic dermatological
disorders, i.e., 11.67% and fungal skin infection, i.e., 8.33%.
The dogs belonging to age group 1-3 years showed greater
susceptibility to non-parasitic dermatological conditions. Breed
wise incidence of pyoderma was found more in the Pomeranian
breed (20.83%), whereas fungal skin affections were found to be
higher in mongrel breed (60.00% and 42.86%, respectively). Male
dogs showed greater involvement in bacterial, fungal, and other
non-parasitic dermatoses. Bacteriological culture examination of
40 pus swabs resulted in the growth of 39 bacterial isolates.
Mycological culture of skin scrapings from 13 suspected cases of
fungal dermatoses resulted in the recovery of five fungal
isolates. Hematological and serum biochemical parameters
revealed a significant difference in all cases of non-parasitic
dermatoses. Histopathological study revealed characteristic
changes like infiltration of neutrophils with perifolliculitis,
hyperkeratosis, and rafts of acantholytic cells. Histochemical
staining revealed purple or magenta color fungal elements.
Conclusion: Based on current experiment it has been
concluded that among non-parasitic dermatoses bacterial and
fungal skin infections are the main ailments, followed by
nutritional and other causes in adult and male dogs which can be
diagnosed by cultural inoculation, microscopic examination of
skin scrapings, and dermatohistopathology along with hematology
and biochemistry.
Keywords: bacterial, canines, fungal,
non-parasitic dermatoses.
References
|
1. Pachauri, S.P. (1999) Canine Preventive Medicine. Nilay
Publication, Pantnagar. p111, 620-631. |
|
|
|
2. Scott, D.W. and Paradis, M. (1990) A survey of canine and
feline skin disorders seen in university practice: Small
Animal Clinic, University of Montreal, Saint Hyacinthe,
Quebec (1987-1988). Can. Vet. J.,31: 753-756. |
|
|
|
3. Feijo, F.M.C., Souza, N.F. de and Ramadinha, R.H.R.
(1998) A study of the yeast Malassezia pachydermatis by
examination of skin cytology in the dog. Rev. Bras. Med.
Vet.,20: 66-68. |
|
|
|
4. Srivastava, A.K. (2011) Staphylococcal pyoderma: An
emerging crisis in canine practice.Indian.J. Canine Pract.,
3(1): 7-12. |
|
|
|
5. Al-Doory, Y. (1980) In: Al-Doory, Y., editor. Laboratory
Medical Mycology. Lea and Febiger, Philadelphia. p249-283. |
|
|
|
6. Scott, D.W., Miller, W.H. Jr. and Griffin, C.E. (1995)
Muller and Kirk's Small Animal Dermatology. 5th ed.
Saunders, W. B, Philadelphia. p218-221, 244-258 and 279-328. |
|
|
|
7. Nesbitt, G.H. (1983) Bacterial skin disease. In: Canine
and Feline Dermatology: A Systematic Approach. Lea and
Febiger, Philadelphia. p81. |
|
|
8. Pal, M. and Singh, D.K. (1983) Studies on dermatomycoses
in dairy animals. Mycoses,26: 317-323.
http://dx.doi.org/10.1111/j.1439-0507.1983.tb03217.x |
|
|
|
9. Culling, C.F.A. (1969) Handbook of Histopathological and
Histochemical Techniques. 3rd ed. Butterworth and Company,
Durban. |
|
|
|
10. Snedecor, G.W. and Cochran, W.G. (1994) Statistical
Methods. 8th ed. USA, The Iowa State University Press. |
|
|
|
11. Aujla, R.S., Singh, N., Sood, N., Gupta, P.P. and Sodhi,
S. (1997) Bacterial dermatitis in dogs in Punjab –
Prevalenceand clinicopathological studies. Indian Vet.
J.,74: 837-840. |
|
|
|
12. Choi, W.P., Lee, S.I. and Lee, K.W. (2000) Aetiological
and epidemiological features of canine dermatitis. Korean J.
Vet. Res.,40: 94-100. |
|
|
|
13. Shyama, V.H. and Vijaykumar, K. (2012) Epidemiological
studies on bacterial skin infection in dogs. J. Vet. Anim.
Sci., 43: 49-51 |
|
|
|
14. Singh, R., Bigh, S.A., Soodan, J.S., Tikoo, A. and
Tantray, H. (2012) Clinico-epidemiological studies in canine
dermatitis. Indian J. Canine Pract., 43: 49-51. |
|
|
|
15. Patil, S.S., Madhava Rao, P. and Patil, N.A. (1999)
Epidemiology and bacterial isolates in canine pyoderma.
Indian J. Vet. Med.,19: 39-40. |
|
|
|
16. Pal, A., Basak, D.N. and Chakrabarty, A. (1993)
Epidemiological studies on canine pyoderma in West Bengal.
Indian J. Anim. Health., 32: 121-125. |
|
|
17. Sparkes, A.H., Gruffyd, J.T.J., Shaw, S.E., Wright, A.I.
and Stokes, C.R. (1993) Epidemiological and diagnostic
features of canine and feline dermatophytosis in the United
Kingdom from 1956-1991. Vet. Rec.,133: 57-61.
http://dx.doi.org/10.1136/vr.133.3.57 |
|
|
|
18. Guilhon, J., Jolivet, G. and Marchand, A. (1974)
Pyodermatitis in dogs (variation in bacterial flora). Bull.
Acad. Vet. Fr.,47: 41-47. |
|
|
|
19. Kelany, W.M. and Galal, H.M. (2011) Diagnosis of
recurrent pyoderma in dogs by traditional and molecular
based diagnostic assays and its therapeutic approach. J.Am.
Sci., 7(3): 120-134. |
|
|
|
20. Manjul, R.S. (2012) Common dermatological disease by
bacteria and fungi in pet dogs.Indian J. Fundam. Appl. Sci.,
2(2): 207-209. |
|
|
21. Yoon, J.W., Lee, K.J., Lee, S.Y., Chae, M.J., Park, J.K.,
Yoo, J.H. and Park, H.M. (2010) Antibiotic resistance
profiles of Staphylococcuspseudintermedius isolates from
canine patients in Korea. J. Microbiol. Biotechnol., 20:
1764-1768.
PMid:21193835 |
|
|
22. T-Sai, Y.J., Chung, W.C., Wang, L.C., Ju, Y.T., Hong,
C.L., T-Sai, Y.Y., Li, Y.H. and Wu, Y.L. (2011) The dog
mite, Demodex canis: Prevalence, fungal co-infection,
reactions to light, and hair follicle apoptosis. J. Insect.
Sci., 11:76.
PMid:21867442 PMCid:PMC3281427 |
|
|
|
23. Nichita, I. and Marcu, A. (2010) The fungal microbiota
isolated from cats and dogs. Anim.Sci. Biotechnol., 43(1):
411-414. |
|
|
|
24. Senthil Kumar, K., Selvaraj, P., Vairamuthu, S.,
Nagarajan, B., Nambi, A.P. and Prathaban, S. (2011) Survey
of fungal isolates from canine mycotic dermatitis in
Chennai. Tamil Nadu J. Vet. Anim. Sci.,7(1): 48-50. |
|
|
|
25. Sharma, S.K., Soodan, J.S. and Sharma, N. (2011) Haemato-biochemical
alterations in canine dermatitis. Indian Vet. J., 88(4):
56-58. |
|
|
26. Schalm, O.W. (1963) Interpretation of leukocyte response
in the dog. J. Am. Vet. Med. Assoc.,142: 147-152.
PMid:13976665 |
|
|
27. Kumar, G.L. and Kiernan, J.A. (2010)Special stains in
dermatopathology. Pathology Education Guide – Special Stains
and H and E. 2nded. Dako North America, California. p200.
PMid:20477865 |
|
