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Research (Published online: 09-10-2015)

1. Relation between antioxidant status and postpartum anestrous condition in Murrah buffalo - Mayukh Ghosh, Meenakshi Gupta, Rajesh Kumar, Sunil Kumar, A. K. Balhara and Inderjeet Singh

Veterinary World, 8(10): 1163-1166



   doi: 10.14202/vetworld.2015.1163-1166


Mayukh Ghosh: Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences,

Hisar - 125 004, Haryana, India;

Meenakshi Gupta: Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences,

Hisar - 125 004, Haryana, India;

Rajesh Kumar: Department of Veterinary Physiology, Veterinary College, Pookode, Lakkidi - 673 576, Kerala, India;

Sunil Kumar: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India;

A. K. Balhara: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffalo, Hisar - 125 001, Haryana,


Inderjeet Singh: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffalo, Hisar - 125 001, Haryana,



Received: 19-04-2015, Revised: 19-08-2015, Accepted: 26-08-2015, Published Online: 09-10-2015


Corresponding author: Rajesh Kumar, e-mail:

Citation: Ghosh M, Gupta M, Kumar R, Kumar S, Balhara AK, Singh I (2015) Relation between antioxidant status and postpartum anestrous condition in Murrah buffalo, Veterinary World 8(10): 1163-1166.

Aim: Objective of the present study was to investigate the relation between antioxidant status and postpartum anestrous (PPA) condition in Murrah buffalo.

Materials and Methods: Jugular blood samples were collected from two different groups of Murrah buffaloes each group consisting of 20 animals. Group I was of PPA and Group II were of cyclic buffaloes. The animals selected were examined for confirmation for cyclic and acyclic condition (>120 days) after calving by routine transrectal ultrasonography. Heard record was also used for cross confirmation.

Results: The analysis of antioxidants in plasma and hemolysates revealed that the levels of vitamin E, β-carotene and reduced glutathione in plasma and superoxide dismutase (SOD) in hemolysate were significantly higher in cyclic animals than PPA animals. The levels of vitamin C, SOD and glutathione peroxidase in plasma did not show any significant difference among the two groups studied. The low antioxidant level in affected animals may predispose them toward PPA condition.

Conclusion: Stress imposed by pregnancy and lactation affected the reproductive performance in PPA animals which might be inherently more susceptible to these stressors than those who were normal cyclic as all the animals were maintained under similar feeding and management practices.

Keywords: antioxidants, postpartum anestrous, stress.

1. FAO. (2004) Agricultural data domain agricultural production, livestock primary, provisional 2005 data for India. Available from: Accessed on 02-04-2015.
2. El-Wishy, A.B. (2007) The postpartum buffalo: II. Acyclicity and anestrus. Anim. Rep. Sci., 97(2-3): 216-236.
3. Kumar, P.R., Shukla, S.N., Shrivastava, O.P. and Purkayastha, R.D. (2013) Incidence of postpartum anestrus among buffaloes in and around Jabalpur. Vet. World, 6: 716-19.
4. Khan, H.M., Bhakat, M., Mohanty, T.K. and Pathbanda, T.K. (2014) Influence of vitamin E, macro and micro minerals on reproductive performance of cattle and buffaloes – A review. Agric. Rev., 35(2): 113-121.
5. Perera, B.M.A.O. (2011) Reproductive cycles of buffalo. Anim. Reprod. Sci., 124: 194-199.
6. Rijasnaz, V.V., Mondal, S.K. and Fahim, A. (2014) Effect of weaning on postpartum reproductive performance ofmurrah buffaloes. Indian J. Anim. Res., 48(5): 501-503.
7. Devaraj, M. and Janakiraman, K. (1986) Postpartum performance of Surti buffaloes. Indian J. Anim. Sci., 56: 532-534.
8. Kumar, R., Gupta, M., Rose, M.K., Ghosh, M. and Singh, I. (2014) Hematological profile in postpartum anestrous and normal cyclic Murrah buffaloes. J. Cell Tissue Res. 14(2): 4241-4243.
9. Ruginosu, E., Creanga, S.T., Sofronie, M., Malancus, R., Boghian, V. and Solcan, G. (2010) Cercetăriagronomicein. Moldova, 2(142): 65-77.
10. Santos, J.E.P., Bisinotto, R.S., Ribeiro, E.S., Lima, F.S., Greco, L.F., Staples, C.R. and Thatcher, W.W. (2010) Applying nutrition and physiology to improve reproduction in dairy cattle. Soc. Reprod. Fertil. Suppl., 67: 387-403.
11. Agrawal, A., Sahel, R.A. and Bedaiwy, M.A. (2003) Role of reactive oxygen species in the pathology of human reproduction. Fertil. Steril., 79: 829-843.
12. Agarwal, A., Gupta, S. and Sharma, R. (2005) Oxidative stress and its implications in female infertility – A clinician's perspective. Reprod. BioMed., 11(5): 641-650 Available from: Accessed on 09-04-2015 .
13. Sreejayan, and Rao, M. N. A. (1997) Nitric Oxide Scavenging by Curcuminoids. J. Pharm. Pharmacol., 49: 105-107.
14. Beutler, E., editor. (1971) Red cell metabolism. In: Manual of Biochemical Methods. Grune and Straton, New York. p146.
15. Madesh, M. and Balasubramanian, K.A. (1998) Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J. Biochem. Biophys., 35(3): 184-188.
16. Kayden, H.J., Chow, C.K. and Bjornson, L.K. (1973) Spectrophotometric method for determination of tocopherol in red blood cells. J. Lipid Res., 14: 533-540.
17. Baker, H., Frank, D. and Winley, N.C. (1968) Clinical Vitaminology. Interscience Publishers, New York. p772.
18. Kahlon, R.S. and Singh, R. (2003) Status of antioxidant enzymes in normal cycling and α-tocopherol supplemented anestrus buffalo heifers (Bubalus bubalis). Asian-Aust. J. Anim. Sci., 16(2): 217-221.
19. Surapaneni, K.M. and Vishnu, P.V. (2009) Lipid peroxidation, glutathione, ascorbic acid, vitamin E, antioxidant enzymes and serum homocystine status in patients with polycystic ovary syndrome. Biol. Med., 1(33): 44-49.
20. Weiss, W.P. (1998) Requirements of fat soluble vitamins for dairy cows: A review. J. Dairy Sci., 81: 2493-2501.
21. Jukola, E., Hakkarainen, J., Saloniemi, H. and Sankari, S. (1996) Blood selenium, vitamin E, vitamin A, and β-carotene concentrations and udder health, fertility treatments, and fertility. J. Dairy Sci., 79: 838-845.
22. Derar, D.R., Hasab-Enaby, H.S., Ali, H.H., Zain, A. and Shehata, S.H. (2011) Postpartum ovarian resumption in native dairy cows in upper Egypt and their relation to oxidant antioxidant status. Endocrinol. Metab. Syndr., S4: 002.
23. Serpek, B., Baspmnar, N., Halmloglu, S. and Erdem, H. (2001) The relationship between ascorbic acid, oestradiol 17β and progesterone in plasma and in ovaries during the sexual cycle in cattle. Rev. Méd. Vét., 152: 253-260.
24. Ahmed, W.A., Nabil, G.M., El-Khadrawy, H.H., Hanafi, E.M. and Abdel-Moez, S.I. (2006) Monitorin progesterone level and markers of oxidative stress in blood of buffalo-cows with impaired fertility. Egypt. J. Biophys. Biomed. Eng., 7: 71-83.
25. Hanafi, E.M., Ahmed, W.M., El Moez, S.I.A., El Khadrawy, H.H. and El Hameed, A.A.A. (2008) Effect of clinical endometritis on ovarian activity and oxidative stress status in Egyptian buffalo-cows. Am. Eurasian J. Agric. Environ. Sci., 4(5): 530-536.
26. Ahmed, W.M., El-Khadrawy, H.H., Amal, R., Hameed, A. and Amer, H.A. (2010) Applied investigation on ovarian inactivity in buffalo-heifers. Int. J. Acad. Res., 2(1): 26-32.
27. Cotgreave, I.A. and Gerdes, R.C. (1998) Recent trends in glutathione biochemistry – Glutathione protein interactions: A molecular link between oxidative stress and proliferation. Biochem. Biophys. Res. Commun., 242: 1-9.
28. Brookes, P.S. (2005) Mitochondrial H+ leak and ROS generation: An old couple. Free Radic. Biol. Med., 38: 12-23.
29. Kumar, B.V.S., Kataria, M. and Kumar, A. (2012) Amelioration of heat stress by feeding electrolytes, ascorbic acid and zinc in buffaloes. Buffalo Bull. 30(4): 229-239.
30. Sharma, N., Singh, N.K., Singh, O.P., Pandey, V. and Verma, P.K. (2011) Oxidative stress and antioxidant status during transition period in dairy cows. Asian-Aust. J. Anim. Sci., 24: 479-484.
31. Amanullah, M., Tandle, M.K., Honnappagol, S.S., Sonwane, S.D., Kartikesh, S.M. Das, B.C. and Jagjiwaniram. (1997) Serum cholesterol, calcium, phosphorus and total protein in relation to estrus and anoestrus in non-descript buffaloes. Indian J. Dairy. Sci., 50: 410-412.
32. Kumar S., Saxena, A. and Ramsagar . (2010) Comparative studies on metabolic profile of anestrous and normal cyclic Murrah buffaloes. Buffalo Bull., 29(1): 7-10.
33. Michalkova, V., Benoit, J.B., Attardo, G.M., Medlock, J. and Aksoy, S. (2014) Amelioration of reproduction-associated oxidative stress in a viviparous insect is critical to prevent reproductive senescence. PLoS One, 9(4): e87554 .
PMid:24763119 PMCid:PMC3998933