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Research (Published online: 28-10-2015)

15. Conventional and fluorescent based semen quality assessment in Karan Fries bulls - A. Panmei, A. K. Gupta, P. R. Shivahre, M. Bhakat and A. Upadhyay

Veterinary World, 8(10): 1243-1246

 

 

   doi: 10.14202/vetworld.2015.1243-1246

 

A. Panmei: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal,

Haryana, India; panmei.achun07@gmail.com

A. K. Gupta: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal,

Haryana, India; guptaak2009@gmail.com

P. R. Shivahre: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal,

Haryana, India; drpr06@gmail.com

M. Bhakat: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal,

Haryana, India; bhakat.mukesh@gmail.com

A. Upadhyay: Division of Dairy Cattle Breeding, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal,

Haryana, India; upadhyay.arpan@gmail.com

 

Received: 30-05-2015, Revised: 10-09-2015, Accepted: 18-09-2015, Published online: 28-10-2015

 

Corresponding author: A. Panmei, e-mail: panmei.achun07@gmail.com


Citation: Panmei A, Gupta AK, Shivahre PR, Bhakat M, Upadhyay A (2015) Conventional and fluorescent based semen quality assessment in Karan Fries bulls, Veterinary World 8(10): 1243-1246.



Aim: The present study was carried out on semen ejaculates of 15 Karan Fries (KF) bulls maintained at Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, India with an objective to evaluate the relationship between the conventional and fluorescent based semen quality analysis of the bulls.

Materials and Methods: A total of 96 ejaculates were collected from 15 KF (Holstein Friesian [HF] crossbred) bulls. Semen were evaluated for color, volume, mass activity (MA) and percentage of individual motility (IM), sperm concentration, percent live spermatozoa, hypo-osmotic swelling test and acrosome integrity, chromatin integrity, sperm viability, and membrane integrity. Data were analyzed using SPSS software package for descriptive analysis. The correlation between rankings of sires based on conventional and fluorescent semen parameters were calculated by Spearman’s rank correlation coefficient.

Results: The average ejaculates volume (ml), sperm concentration (106/ml), MA, IM (%), live (%), morphological abnormalities (%), host (%), acrosome integrity (%), chromomycin A3 (CMA3) (%), SYBR-PI (%), and fluorescent isothiocyanate-peanut agglutinin (FITC-PNA) (%) were 4.57±0.36, 1162.98±97.93, 2.95±0.09, 60.8±1.22, 71.41±2.10, 9.31±1.15, 65.5±1.81, 86.6±1.59, 3.53±0.43, 65.39±2.23 and 74.47±2.53, respectively. Rank correlations were found to be significant for SYBR-PI and FITC-PNA with most of the parameters evaluated by conventional methods. Overall, among conventional criteria, IM revealed ranking of bulls almost similar to that of fluorescent criteria.

Conclusion: Overview of our results indicated that, among conventional criteria, MA and IM revealed ranking of bulls almost similar to that of fluorescent criteria.

Keywords: conventional method, fluorescent technique, Karan Fries, semen quality.



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