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R esearch
(Published online:
22-09-2015)
12. Emergence
of oriental theileriosis in cattle and its transmission through
Rhipicephalus (Boophilus) microplus in Assam, India -
Parikshit
Kakati, Prabhat Chandra Sarmah, Debdatta Ray, Kanta Bhattacharjee,
Rajeev Kumar Sharma, Luit Moni Barkalita, Dipak Kumar Sarma,
Bhaben Chandra Baishya, Pranjal Borah and Bobitha Stanley
Veterinary World, 8(9): 1099-1104
doi:
10.14202/vetworld.2015.1099-1104
Parikshit Kakati:
Department of Parasitology, College of Veterinary Science, Assam
Agricultural University, Guwahati, Assam, India; parik67@gmail.com
Prabhat
Chandra Sarmah:
Department of Parasitology, College of Veterinary Science, Assam
Agricultural University, Guwahati, Assam, India; pcsarmah@gmail.com
Debdatta Ray:
Division of Parasitology, Indian Veterinary Research Institute,
Izatnagar, Uttar Pradesh, India; dd_rayvet@yahoo.co.in
Kanta
Bhattacharjee:
Department of Parasitology, College of Veterinary Science, Assam
Agricultural University, Guwahati, Assam, India; kantabhatta@gmail.com
Rajeev
Kumar Sharma:
Department of Microbiology, College of Veterinary Science, Assam
Agricultural University, Assam, India; dr.sharmark@rediffmail.com
Luit
Moni Barkalita:
Department of Animal Biotechnology, College of Veterinary Science,
Assam Agricultural University, Assam, India; luitbarkalita@gmail.com
Dipak
Kumar Sarma:
Teaching Veterinary Clinical Complex, College of Veterinary
Science, Assam Agricultural University, Assam, India; drdipak77@gmail.com
Bhaben
Chandra Baishya:
Teaching Veterinary Clinical Complex, College of Veterinary
Science, Assam Agricultural University, Assam, India;
bhabenchbaishya@gmail.com
Pranjal
Borah:
Goat Research Station, Burnihat, Assam Agricultural University,
Assam, India; drpran2002@yahoo.co.in
Bobitha
Stanley: Division of Parasitology, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India;
bobitha916@gmail.com
Received: 01-06-2015, Revised: 12-08-2015, Accepted: 20-08-2015,
Published online: 22-09-2015
Corresponding author:
Kanta Bhattacharjee, e-mail: kantabhatta@gmail.com
Citation:
Kakati P, Sarmah PC,
Ray D, Bhattacharjee K, Sharma RK, Barkalita LM, Sarma DK, Baishya
BC, Borah P, Stanley B (2015) Emergence of oriental theileriosis
in cattle and its transmission through Rhipicephalus (Boophilus)
microplus in Assam, India, Veterinary World 8(9):
1099-1104.
Abstract
Aim:
The aim of the present study was to investigate the presence
of Theileria in blood samples of crossbred and indigenous
adult cows raised under unorganized small scale farming system in
a Babesia and Anaplasma endemic geographical area
from Assam, India and to see its transmission through
Rhipicephalus (Boophilus) microplus ticks.
Materials and Methods: For the present study, 57 clinical
cases of cattle suspected to be of hemoparasitic infections were
taken into consideration. The parasites were identified based on
morphology in giemsa stained blood smear followed by polymerase
chain reaction (PCR). Sera samples were tested for T. annulata
antibodies in plate and Dot-ELISA. PCR was also conducted in
eggs of Rhipicephalus (Boophilus) microplus tick collected
from a Theileria orientalis positive animal.
Results: PCR amplified 1124, 776, and 160 bp DNA fragments of
B. bigemina (64.91%),T. orientalis(21.05%)
and A. marginale (14.03%), respectively. This assay further
conducted in 12 T. orientalis positive blood samples with
primers of Buffeli, Chitose, and Ikeda variants of T.
orientalis showed 3 samples positive to Ikeda type and none
for Buffeli and Chitose. Babesia bovis and Theileria
annulata specific primers also did not amplify any fragment
during the PCR assay of the blood samples. Further, all sera
samples tested negative to T. annulata antibodies in Plate
and Dot-ELISA. PCR conducted in eggs of R (B).microplus
tick collected from a T. orientalis positive animal
revealed presence of the parasite DNA. Gradual improvement in
physical condition leading to complete recovery in 10 out of 12
T. orientalis infected clinical cases treated with
buparvaquone(at 2.5mg/kg.b.wt I/M) was the feedback obtained from
field veterinarians and the cattle owners.
Conclusion: The present investigation represents the first
report of occurrence of T. orientalis in cattle of Assam
with involvement of pathogenic Ikeda strain in clinical outbreaks
and its possible natural transmission by R (B). microplus
through the transovarian mode.
Keywords: Anaplasma marginale, Assam (India),
Babesia bigemina, Ikeda type, Rhipicephalus (Boophilus)
microplus, Theileria orientalis.
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