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R esearch
(Published online:
22-09-2015)
13. Assessment
of blood changes post-challenge with Corynebacterium
pseudotuberculosis and its exotoxin (phospholipase D): A
comprehensive study in goat
- Z. K. H. Mahmood, F.
F. Jesse, A. A. Saharee, S. Jasni, R. Yusoff and H. Wahid
Veterinary World, 8(9): 1105-1117
doi:
10.14202/vetworld.2015.1105-1117
Z. K.
H. Mahmood:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, Serdang,
Selangor,
Malaysia; zaid.jeber@Gmail.com
F. F.
Jesse:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, Serdang,
Selangor,
Malaysia; jesseariasamy@gmail.com
A. A.
Saharee:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, Serdang,
Selangor,
Malaysia; abdaziz@upm.edu.my
S.
Jasni:
Department of Microbiology and Pathology, Faculty of Veterinary
Medicine, Universiti Malaysia Kelantan, Malaysia; jasni@umk.edu.my
R.
Yusoff:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, Serdang,
Selangor,
Malaysia; rosnina@vet.upm.edu.my
H.
Wahid: Department of Veterinary Clinical Studies, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, Serdang,
Selangor,
Malaysia;
wahidharon@gmail.com
Received: 23-03-2015, Revised: 16-08-2015, Accepted: 22-08-2015,
Published online: 22-09-2015
Corresponding author:
F. F. Jesse, e-mail: jesseariasamy@gmail.com
Citation:
Mahmood ZKH, Jesse FF,
Saharee AA, Jasni S, Yusoff R, Wahid H (2015) Assessment of blood
changes post-challenge with Corynebacterium pseudotuberculosis
and its exotoxin (phospholipase D): A comprehensive study in
goat, Veterinary World 8(9): 1105-1117.
Abstract
Aim:
There is very little information regarding blood changes
during the challenge of phospholipase D (PLD) in goats. Therefore,
this experiment was conducted to study the changes in blood after
the challenge with Corynebacterium pseudotuberculosis and
its exotoxin, PLD to fill in the gap of caseous lymphadenitis (CLA)
research.
Materials and Methods: Twenty-six crossbred Boer goats aged
12-14 months were divided into 3 groups; the first group n=6 was
inoculated with 1 ml phosphate buffered solution s.c. as the
control. The second group n=10 was inoculated with C.
pseudotuberculosis 1 × 10 9 cfu s.c. The
third group n=10 was intravenous injected with PLD 1 ml/20 kg body
weight. Serial blood collections were done at 1 h, 3 h, 5 h, 8 h,
and 12 h then every 24 h post-inoculation for the first 30 days of
the experiment. Subsequently, the blood collection continued twice
a week till the end of the experiment (90 days post-challenge).
Results: Both C. pseudotuberculosis and PLD treated
groups showed significant changes (p<0.05) in red blood cell
count, hemoglobin (Hb), packed cell volume, mean corpuscular
volume, mean corpuscular Hb concentration, white blood cell count,
neutrophils, lymphocytes, monocytes, eosinophils, basophils,
globulin, and total plasma proteins. Similarly, both treated
groups showed significant changes (p<0.05) in alanine transaminase,
alkaline phosphatase, aspartate transaminase, total bilirubin,
calcium concentration, creatine phosphokinase, creatinine, gamma-glutamyl
transpeptidase, urea concentration, lactate dehydrogenase,
prothrombin time, and activated partial thromboplastin time.
Conclusion: It concluded that C. pseudotuberculosis and
PLD have a negative impact on the goat’s health in general
reflected by all those changes recorded in the hemogram, leukogram,
and the blood chemistry.
Keywords: blood biochemistry, blood changes,
caseous lymphadenitis, goat, Corynebacterium pseudotuberculosis,
hemogram, leukogram, phospholipase D.
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