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Research (Published online: 08-09-2015)

2.  Experimental infection of Aphanomyces invadans and susceptibility in seven species of tropical fish - Seyedeh F. Afzali, Hassan Hj. Mohd Daud, Issa Sharifpour, Mohammad Afsharnasab and Shiv Shankar

Veterinary World, 8(9): 1038-1044



   doi: 10.14202/vetworld.2015.1038-1044


Seyedeh F. Afzali: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM,

Serdang, Selangor, Malaysia;

Hassan Hj. Mohd Daud: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM,

Serdang, Selangor, Malaysia;

Issa Sharifpour: Iranian Fisheries Research Organization, Tehran, Iran;

Mohammad Afsharnasab: Iranian Fisheries Research Organization, Tehran, Iran;

Shiv Shankar: Department of Food Engineering, Mokpo National University, Korea;


Received: 07-04-2015, Revised: 18-07-2015, Accepted: 26-07-2015, Published online: 08-09-2015


Corresponding author: Hassan Hj. Mohd Daud, e-mail:

Citation: Afzali S.F, Hassan M.D , Sharifpour I, Afsharnasab M, Shankar S (2015) Experimental Infection of Aphanomyces invadans and susceptibility in seven species of Tropical fish, Veterinary World 8(9): 1038-1044.

Aim: Epizootic ulcerative syndrome (EUS) causes by aquatic oomycete fungus, Aphanomyces invadans is a dangerous fish disease of a wide range of fresh and brackish water, wild and farmed fish throughout the world. The objective of the present study was to determine the susceptibility of a number of tropical fish species to the EUS and compare the severity of infection between experimental groups.

Materials and Methods: Snakehead, Channa striata (Bloch, 1793); snakeskin gourami, Trichopodus pectoralis (Regan, 1910); koi carp, Cyprinus carpio (Linnaeus, 1758); broadhead catfish, Clarias macrocephalus (GŁnther, 1864); goldfish, Carassius auratus (Linnaeus, 1758); climbing perch, Anabas testudineus (Bloch, 1792); and Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) were challenged by intramuscular injection using zoospores of Aphanomyces invadans (NJM9701). The infected fish skins and muscles were examined for EUS histopathological characteristics, and the results on the severity of lesions and mortality were analyzed using SPSS program.

Results: All zoospore-injected fish were shown to be susceptible to the EUS infection except Nile tilapia. Although, the general histopathological pattern was similar in the zoospore-injected group, but there were some variation in granulomatous reaction, that is the presence or absence of giant cells, and time of mortality were detected. The result of statistical analysis showed that there was a significant difference between species, (c2=145.11 and p<0.01).

Conclusion: Gourami, koi carp, and catfish were demonstrated to be highly susceptible while goldfish and climbing perch were found to be moderately susceptible to the EUS infection. These findings suggested that the cellular response of fish to mycotic infection and granulomatous reaction varied in different fish species, which could not be an indicator of susceptibility or resistant to the EUS itself, although it was shown that the granulation rate and the level of maturity or solidification (consolidation of granulomas) were higher in resistant fish.

Keywords: Aphanomyces invadans, epizootic ulcerative syndrome, freshwater fish, histopathology.

1. OIE. (2014). Infection with Aphanomyces invadans (Epizootic Ulcerative Syndrome). Ch. 10.2. Aquatic Animal Health Code - 07/08/2014. Available from: Accessed on: 25-03-2015
2. Lilley, J., Callinan, R.B., Chinabut, S. and Kanchanakhan, S. (1998) Epizootic Ulcerative Syndrome (EUS) Technical Handbook. The Aquatic Animal Health Research Institute, Bangkok. p88.
3. Baldock, F.C., Blazer, V., Callinan, R., Hatai, K., Karunasagar, I., Mohan, C.V. and Bondad-Reantaso, M.G. (2005) Outcomes of a short expert consultation on epizootic ulcerative syndrome (EUS): Re-examination of causal factors, case definition and nomenclature. In: Walker, P., Laster, R. and Bondad-Reantaso, M.G., editors. Asian Fisheries Society Publications, Manila. p555-585.
4. Saylor, R.K., Miller, D.L., Vandersea, M.W., Bevelhimer, M.S., Schofield, P.J. and Bennett, W.A. (2010). Epizootic ulcerative syndrome caused by Aphanomyces invadans in captive bullseye snakehead Channa marulius collected from south Florida, USA. Dis. Aquat. Organ., 88: 169-175.
5. Kamilya, D. and Baruah, A. (2013) Epizootic ulcerative syndrome (EUS) in fish: History and current status of understanding. Rev. Fish Biol Fish., 24: 1-12.
6. OIE. (2013). Infection with Aphanomyces invadans, Epizootic Ulcerative Syndrome. Manual of Diagnostic Tests for Aquatic Animals 2013. Ch. 2.3.2. OIE Aquatic Animal Health Standards Commission, World Assembly of Delegates of OIE, Paris, France. p13.
7. Roberts, R.J. (2012) Fish Pathology. 4th ed. Wiley-Blackwell, London. p590.
8. Johnson, R.A., Zabrecky, J., Kiryu, Y. and Shields, J.D. (2004) Infection experiments with Aphanomyces invadans in four species of estuarine fish. J. Fish Dis., 27: 287-295.
9. Dykstra, M.J., Levine, J.F., Noga, E.J., Hawkins, J.H., Gerdes, P., Grier, H. and Strake, D. (1989) Ulcerative mycosis: A serious menhaden disease of the southeastern coastal fisheries of the United States. J. Fish Dis., 12: 175-178.
10. Pradhan, P.K., Mohan, C.V., Shankar, K.M. and Mohana Kumar, B. (2008) Infection experiments with Aphanomyces invadans in advanced fingerlings of four different carp species. Proceeding of the Diseases in Asian Aquaculture VI, Fish Health Section, Manila, Philippines, 2008. Asian Fisheries Society, Manila, Philippines. p105-114.
11. Baruah, A., Saha, R.K. and Kamilya, D. (2012) Inter-Species transmission of the epizootic ulcerative syndrome (EUS) pathogen, Aphanomyces invadans, and associated physiological responses. Isr. J Aquac., 696: 9.
12. Oidtmann, B., Steinbauer, P., Geiger, S. and Hoffmann, R.W. (2008) Experimental infection and detection of Aphanomyces invadans in European catfish, rainbow trout and European eel. Dis. Aquat. Organ., 82: 195-207.
13. Sharifpour, I. (1997) Histology of the Inflammatory Response of Carp (Cyprinus carpio L.) to Various Stimuli. Doctoral Dissertation, University of Stirling, UK.
14. Wada, S., Rha, S., Kondo, T., Suda, H., Hatai, K. and Ishi, H. (1996). Histopathology of ayu and carp artificially infected with Aphanomyces piscicida. Fish Pathol., 31: 71-80.
15. Chinabut, S., Roberts, R.J., Willoughby, G.R. and Pearson, M.D. (1995) Histopathology of snakehead, Channastriatus (Bloch), experimentally infected with the specific Aphanomyces fungus associated with epizootic ulcerative syndrome (EUS) at different temperatures. J. Fish Dis., 18: 41-47.
16. Wada, S., Yuasa, K., Rha, S., Nakamura, K. and Hatai, K. (1994) Histopathology of Aphanomyces infection in dwarf gourami "Colisa lalia". Fish Pathol., 29: 229-237.
17. Thompson, K.D., Lilley, J.H., Chen, S.C., Adams, A. and Richards, R.H. (1999) The immune response of rainbow trout (Oncorhynchus mykiss) against Aphanomyces invadans. Fish Shellfish Immunol., 9: 195-201.
18. Pradhan, P.K., Mohan, C.V, Shankar, K.M. and Kumar, B.M. (2007) Sequential inflammatory response of fingerlings of Indian major carps to Aphanomyces invadans. Indian J. Fish., 54: 389-396.
19. Phadee, P., Kurata, O., Hatai, K., Hirono, I. and Aoki, T. (2004) Detection and identification of fish-pathogenic Aphanomyces piscicida using polymerase chain reaction (PCR) with species-specific primers. J. Aquat. Anim. Health, 16: 220-230.