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R esearch
(Published online:
08-09-2015)
2.
Experimental infection of Aphanomyces invadans and
susceptibility in seven species of tropical fish -
Seyedeh F. Afzali,
Hassan Hj. Mohd Daud, Issa Sharifpour, Mohammad Afsharnasab and
Shiv Shankar
Veterinary World, 8(9): 1038-1044
doi:
10.14202/vetworld.2015.1038-1044
Seyedeh F. Afzali:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 UPM,
Serdang,
Selangor, Malaysia; s.f.a.845@gmail.com
Hassan Hj. Mohd Daud:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 UPM,
Serdang,
Selangor, Malaysia; hassanmd@upm.edu.my
Issa
Sharifpour:
Iranian Fisheries Research Organization, Tehran, Iran;
isharifpour@yahoo.com
Mohammad Afsharnasab:
Iranian Fisheries Research Organization, Tehran, Iran;
mafsharnasab@yahoo.com
Shiv
Shankar: Department of Food Engineering, Mokpo National
University, Korea;
shivbiotech@gmail.com
Received: 07-04-2015, Revised: 18-07-2015, Accepted: 26-07-2015,
Published online: 08-09-2015
Corresponding author:
Hassan Hj. Mohd Daud, e-mail: hassanmd@upm.edu.my
Citation:
Afzali S.F, Hassan M.D
, Sharifpour I, Afsharnasab M, Shankar S (2015) Experimental
Infection of Aphanomyces invadans and susceptibility in
seven species of Tropical fish, Veterinary World 8(9):
1038-1044.
Abstract
Aim:
Epizootic ulcerative syndrome
(EUS) causes by aquatic oomycete fungus,
Aphanomyces invadans is a
dangerous fish disease of a wide range of fresh and brackish
water, wild and farmed fish throughout the world. The objective of
the present study was to determine the susceptibility of a number
of tropical fish species to the EUS and compare the severity of
infection between experimental groups.
Materials and Methods:
Snakehead, Channa striata
(Bloch, 1793); snakeskin gourami,
Trichopodus pectoralis
(Regan, 1910); koi carp,
Cyprinus carpio
(Linnaeus, 1758); broadhead catfish,
Clarias macrocephalus
(Günther, 1864); goldfish,
Carassius auratus
(Linnaeus, 1758); climbing perch,
Anabas testudineus
(Bloch, 1792); and Nile tilapia,
Oreochromis niloticus
(Linnaeus, 1758) were challenged by
intramuscular injection using zoospores of
Aphanomyces invadans
(NJM9701). The infected fish skins and muscles were examined for
EUS histopathological characteristics, and the results on the
severity of lesions and mortality were analyzed using SPSS
program.
Results: All
zoospore-injected fish were shown to be susceptible to the EUS
infection except Nile tilapia. Although, the general
histopathological pattern was similar in the zoospore-injected
group, but there were some variation in granulomatous reaction,
that is the presence or absence of giant cells, and time of
mortality were detected. The result of statistical analysis showed
that there was a significant difference between species, (c 2=145.11
and p<0.01).
Conclusion: Gourami, koi carp,
and catfish were demonstrated to be highly susceptible while
goldfish and climbing perch were found to be moderately
susceptible to the EUS infection. These findings suggested that
the cellular response of fish to mycotic infection and
granulomatous reaction varied in different fish species, which
could not be an indicator of susceptibility or resistant to the
EUS itself, although it was shown that the granulation rate and
the level of maturity or solidification (consolidation of
granulomas) were higher in resistant fish.
Keywords: Aphanomyces invadans,
epizootic ulcerative syndrome,
freshwater fish, histopathology.
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