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Research (Published online: 02-04-2016)

1. Pattern of induced estrus and conception rate following Ovsynch and Ovsynch based gonadotropin-releasing hormone treatments initiated on day 6 of estrous cycle in repeat breeding crossbred cows - N. Ahmed, D. Kathiresan, F. A. Ahmed, K. Lalrintluanga, P. Mayengbam and J. M. Gali

Veterinary World, 9(4): 342-345

 

 

   doi: 10.14202/vetworld.2016.342-345

 

 

N. Ahmed: Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Aizawl, Mizoram, India; nekibahmeds@gmail.com

D. Kathiresan: Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Aizawl, Mizoram, India; kathiresanvet@yahoo.com

F. A. Ahmed: Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Aizawl, Mizoram, India; drfazalali@gmail.com

K. Lalrintluanga: Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Aizawl, Mizoram, India; drtluanga40@yahoo.co.in

P. Mayengbam: Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Aizawl, Mizoram, India; dr_prava@rediffmail.com

J. M. Gali: Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Aizawl, Mizoram, India; biochemjagan@gmail.com

 

Received: 28-10-2015, Revised: 13-02-2016, Accepted: 24-02-2016, Published online: 02-04-2016

 

Corresponding author: N. Ahmed, e-mail: nekibahmeds@gmail.com


Citation: Ahmed N, Kathiresan D, Ahmed FA, Lalrintluanga K, Mayengbam P, Gali JM (2016) Pattern of induced estrus and conception rate following Ovsynch and Ovsynch based gonadotropin-releasing hormone treatments initiated on day 6 of estrous cycle in repeat breeding crossbred cows, Veterinary World, 9(4): 342-345.



Aim: The aim was to evaluate the estrus response, incidence of accessory corpus luteum formation and fertility following different hormonal protocols in repeat breeding crossbred cows.

Materials and Methods: This study was carried out on 24 repeat breeding crossbred cows allotted into four groups. Cows of Group I was not given any treatment, Group II was treated with gonadotropin-releasing hormone (GnRH) injection on day 6 post-estrus, Group III was treated with Ovsynch protocol, and Group IV was treated with Ovsynch based GnRH treatment. Estrus responses such as duration, onset, percentage, and intensity of estrus were recorded during the study. The incidence of accessory corpus luteum was recorded per rectally on day 7 after first and additional GnRH of Ovsynch treatment. The conception rate for all groups was calculated by the absence of estrus and on day 45 after artificial insemination (AI) per rectum. Serum samples were collected at AI and day 12 post-AI in Group I and II. Serum samples were also collected at GnRH, Prostaglandin F2α (PGF2α), timed AI (TAI) and day 12 post-TAI in Group III and IV.

Results: Ovsynch and Ovsynch based GnRH treatments are resulted in 100.00% induction of estrus after the PGF2α injection. Onset of induced estrus after the PGF2α injection for Group III and IV was recorded as 48.750±0.713 and 51.472±1.989 h, respectively, and it was not significant. There was no significant difference in duration of estrus among the groups. The incidence of intermediate estrus intensity was found to be highest. All the cows showed the incidence of formation of accessory corpus luteum subsequent to GnRH treatment on day 6 of the estrous cycle in Group II, III, and IV. The conception rate was 0.00%, 16.67%, 50.00%, and 50.00% in Group I, II, III, and IV, respectively.

Conclusion: Ovsynch and Ovsynch based GnRH treatments initiated on day 6 of estrous cycle capable of responding with a higher percentage of ovulation and formation of accessory corpus luteum which helped in higher conception rate over single post-AI GnRH treatment in repeat breeders. These treatments responded with better estrus response but did not significantly improve estrus intensity.

Keywords: accessory corpus luteum, conception rate, estrus response, Ovsynch, repeat breeding.



1. Saxena, M.S. (2004) Repeat breeding in cows and buffaloes. In: Proceeding National Symposium on Conservation and Propagation of Indigenous Breeds of Cattle and Buffaloes, Feb. Pantnagar, Uttaranchal. p26-28.
 
2. Patel, K.R., Dhami, A.J., Hadiya, K.K. and Savalia, K.K. (2014) Effect of mid-cycle PGF2α and GnRH at AI on conception rate, plasma progesterone and biochemical profile in repeat breeding crossbred cows. Indian J. Field Vet., 9(3): 5-11.
 
3. Kim, I.H., Suh, G.H. and Son, D.S. (2003) A progesterone-based timed AI protocol more effectively prevents premature estrus and incomplete luteal regression than an Ovsynch protocol in lactating Holstein cows. Theriogenology, 60: 809-817.
http://dx.doi.org/10.1016/S0093-691X(03)00136-5
 
4. Satheshkumar, S., Palanisamy, A., Ramadass, P., Subramanian, A. and Kathiresan, D. (2008) Follicular wave synchronization using GnRH agonist in Jersey crossbred cows. Indian J. Anim. Reprod., 29(2): 154-158.
 
5. Satheshkumar, S., Subramanian, A., Devanathan, T.G., Kathiresan, D., Veerapandian, C. and Palanisamy, A. 7(2012) Follicular and endocrinological turnover associated with GnRH induced follicular wave synchronization in Indian crossbred cows. Theriogenology, 77: 1144-1150.
http://dx.doi.org/10.1016/j.theriogenology.2011.10.019
PMid:22192396
 
6. Beltran, M.P. and Vasconcelos, J.L.M. (2008) Conception rate in Holstein cows treated with GnRH or hCG on the fifth day post artificial insemination during summer. Arq. Bras. Med. Vet. Zootec., 60: 580-586.
http://dx.doi.org/10.1590/S0102-09352008000300009
 
7. Kulasekar, K., Ranagasamy, S., Satheshkumar, S. and Sathiamoorthy, T. (2012) Induction of accessory corpus luteum using GnRH and fertility rate in repeat breeding cows. National Symposium on Addressing Animal Reproductive Stresses through Biotechnological Tools. 21-23 November, Assam, India. p90.
 
8. Pursley, J.R., Mee, M.O. and Wiltbank, M.C. (1995) Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology, 44: 915-923.
http://dx.doi.org/10.1016/0093-691X(95)00279-H
 
9. Rao, S.V. and Rao, A.R. (1981) Estrous behaviour and ovarian activity of crossbred heifers. Indian Vet. J., 58: 881-884.
 
10. Vijayarajan, A., Chandrahasan, C. and Napolean, R.E. (2009) Synchronization of ovulation in repeat breeding crossbred cows. Indian J. Field Vet., 5(1): 57-58.
 
11. Biradar, S., Tandle, M.K., Haribabu, Y., Usturge, S.M., Patil, N.A. and Suranagi, M.D. (2014) Study on efficacy of Co-Synch and Ovsynch protocol on fertility in repeat breeder buffaloes. Indian J. Adv. Plant Res., 1(5): 1-3.
 
12. Kasimanickam, R., Cornwell, J.M. and Nebel, R.L. (2005) Fertility following fixed-time AI or insemination at observed estrus in Ovsynch and Heatsynch programs in lactating dairy cows. Theriogenology, 63: 2550-2559.
http://dx.doi.org/10.1016/j.theriogenology.2004.11.003
PMid:15910934
 
13. Carabă, I. and Velicevici, S. (2013) Using Ovsynch protocol versus Cosynch protocol in dairy cows. Anim. Sci. Biotechol., 46(2): 63-65.
 
14. Velladurai, C., Napolean, R.E., Selvaraju, M. and Doraisamy, K.A. (2014) Pattern of induced estrus and conception rate following Ovsynch programme in postpartum dairy cows. Indian J. Field Vet., 10(2): 23-25.
 
15. Chaudhary, A.K., Panchal, M.T., Shah, R.G. and Dhami, A.J. (2012) Effect of Ovsynch and one shot PGF2α protocols on estrus synchronization, plasma progesterone and conception rate in cyclic crossbred cows under field conditions. National Symposium on Addressing Animal Reproductive Stresses through Biotechnological Tools. November 21-23, Assam, India. p71.
 
16. Bhat, F.A. and Bhattacharyya, H.K. (2012) Estrus duration and status of reproductive organs in repeat breeding cows. Iran. J. Appl. Anim. Sci., 2(3): 295-299.
 
17. Alyas, M., Razzaque, W.A.A., Ali, R., Rao, M.M., Kumar, S., Bharadwaj, H.R. and Hussain, K. (2013) Supplementation of progesterone in Ovsynch to improve fertility in post-partum anestrus buffaloes. Int. J. Adv. Res., 1(5): 79-82.
 
18. Pursley, R. and Martins, J.P. (2011) Enhancing fertility of lactating dairy cows. Mich. Dairy Rev., 16(2): 1-3.
 
19. Foster, J.P., Lamming, G.E. and Peters, A.R. (1980) Short-term relationships between plasma LH, FSH and progesterone concentrations in post-partum dairy cows and the effect of GnRH injection. J. Reprod. Fertil., 59: 321-327.
http://dx.doi.org/10.1530/jrf.0.0590321
PMid:6776270
 
20. Keskin, A., Gumen, A., Mecitoglu, G.Y., Karakaya, E., Tasdemir, U., Celik, Y. and Okut, H. (2010) The progesterone based Ovsynch protocol and GnRH treatment after artificial insemination on conception rate in repeat breeder cows. Uludag Univ. J. Fac. Vet. Med., 29(2): 65-70.
 
21. Singh, K.P., Singh, B., Singh, S.V., Singh, J.P., Singh, P. and Singh, H.N. (2014) Efficacy of hormonal interventions on conception rate of repeat breeding crossbred cows. Indian Vet. J., 91(02): 34-35.
 
22. Gaja, A.O., Hamana, K., Kubota, C. and Kojima, T. (2008) Evaluation of a third GnRH injection administered six days after the 2nd GnRH injection of Ovsynch on the reproductive performance of Japanese black cows. J. Vet. Sci. 9(3): 273-279.
http://dx.doi.org/10.4142/jvs.2008.9.3.273
PMCid:PMC2811839
 
23. Vasconcelos, J.L.M., Silcox, R.W., Rosa, G.J.M., Pursley, J.R. and Wiltbank, M.C. (1999) Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. Theriogenology, 52: 1067-1078.
http://dx.doi.org/10.1016/S0093-691X(99)00195-8
 
24. Martins, J.N.P., Policelli, R.K., Neuder, L.M., Raphael, W. and Pursley, J.R. (2011) Effects of cloprostenol sodium at final prostaglandin F-2 alpha of Ovsynch on complete luteolysis and pregnancy per artificial insemination in lactating dairy cows. J. Dairy Sci., 94: 2815-2824.
http://dx.doi.org/10.3168/jds.2010-3652
PMid:21605751
 
25. Bello, N.M., Steibel, J.P. and Pursley, J.R. (2006) Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of Ovsynch in lactating dairy cows. J. Dairy Sci., 89: 3413-3424.
http://dx.doi.org/10.3168/jds.S0022-0302(06)72378-5
 
26. Keith, B.R., Leslie, K.E., Johnson, W.H. and Walton, J.S. (2005) Effect of presynchronization using prostaglandin F2α and a milk-ejection test on pregnancy rate after the timed artificial insemination protocol, Ovsynch. Theriogenology, 63: 722-738.
http://dx.doi.org/10.1016/j.theriogenology.2004.03.023
PMid:15629792