Veterinary World

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Research (Published online: 28-04-2016)

14. In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage - Norisal Binti Nasai, Yusuf Abba, Faez Firdaus Jesse Abdullah, Murugaiyah Marimuthu, Abdulnasir Tijjani, Muhammad Abubakar Sadiq, Konto Mohammed, Eric Lim Teik Chung and Mohammed Ariff Bin Omar

Veterinary World, 9(4): 417-420

 

 

   doi: 10.14202/vetworld.2016.417-420

 

 

Norisal Binti Nasai: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; usuba5050@live.com

Yusuf Abba: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; yabbavet@gmail.com

Faez Firdaus Jesse Abdullah: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Research Centre for Ruminant Disease, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; jesseariasamy@gmail.com

Murugaiyah Marimuthu: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; researchofficer2002@yahoo.com

Abdulnasir Tijjani: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; nasirvet69@gmail.com

Muhammad Abubakar Sadiq: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; masadiqvet@gmail.com

Konto Mohammed: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; kontomohammed@gmail.com

Eric Lim Teik Chung: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; ericlim88@gmail.com

Mohammed Ariff Bin Omar: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Research Centre for Ruminant Disease, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; mo_ariff@upm.edu.my

 

Received: 20-10-2015, Accepted: 16-03-2016, Published online: 28-04-2016

 

Corresponding author: Faez Firdaus Jesse Abdullah, e-mail: jesseariasamy@gmail.com


Citation: Nasai NB, Abba Y, Abdullah FFJ, Marimuthu M, Tijjani A, Sadiq MA, Mohammed K, Chung ELT, Omar MAB (2016) In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage, Veterinary World, 9(4): 417-420.



Aim: Gastrointestinal helminthosis is a global problem in small ruminant production. Most parasites have developed resistance to commonly available anthelminthic compounds, and there is currently an increasing need for new compounds with more efficacies. This study evaluated the in vitro effects of ethanolic extract of Curcuma longa (EECL) as a biological nematicide against third stage Haemonchus larvae (L3) isolated from sheep.

Materials and Methods: Haemonchus L3 were cultured and harvested from the feces of naturally infected sheep. EECL was prepared and three concentrations; 50, 100, and 200 mg/mL were tested for their efficacies on Haemonchus L3. Levamisole at concentration 1.5 and 3 mg/mL were used as positive controls.

Results: EECL showed anthelmintic activity in a dose-dependent manner with 78% worm mortality within 24 h of exposure at the highest dose rate of 200 mg/mL. There was a 100% worm mortality rate after 2 h of levamisole (3 mg/mL) admisntration. However, there was a comparable larvicidal effect between when levamisole (1.5 mg/mL) and EECL (200 mg) were administered.

Conclusion: The study shows that EECL does exhibit good anthelmintic properties at 200 mg/mL which is comparable with levamisole at 1.5 mg/mL.

Keywords: Curcuma longa, ethanolic extract, Haemonchus, larvae 3, levamisole, strongyle.



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