Open Access
Research
(Published
online: 28-04-2016)
15.
Polymorphism in spa gene
of Staphylococcus aureus from bovine subclinical mastitis
-
Taruna Bhati,
Prerna Nathawat,
Sandeep Kumar Sharma,
Rahul Yadav, Jyoti Bishnoi
and Anil Kumar Kataria
Veterinary World, 9(4): 421-424
doi:
10.14202/vetworld.2016.421-424
Taruna Bhati :
Department of
Veterinary Microbiology and Biotechnology, College of Veterinary
and Animal Science, Rajasthan University of Veterinary and
Animals Sciences, Bikaner, Rajasthan, India; vetcvas.bhati@gmail.com
Prerna Nathawat :
Department of
Veterinary Microbiology and Biotechnology, College of Veterinary
and Animal Science, Rajasthan University of Veterinary and
Animals Sciences, Bikaner, Rajasthan, India; nathawatprerna@gmail.com
Sandeep Kumar Sharma :
Department of
Veterinary Microbiology and Biotechnology, Post Graduate
Institute of Veterinary Education and Research, Rajasthan
University of Veterinary and Animals Sciences, Bikaner,
Rajasthan, India: drsharmask01@gmail.com
Rahul Yadav :
Department of
Veterinary Microbiology and Biotechnology, College of Veterinary
and Animal Science, Rajasthan University of Veterinary and
Animals Sciences, Bikaner, Rajasthan, India; drrahul16889@gmail.com
Jyoti Bishnoi :
Department of
Veterinary Microbiology and Biotechnology, College of Veterinary
and Animal Science, Rajasthan University of Veterinary and
Animals Sciences, Bikaner, Rajasthan, India; jyotibishnoi88@gmail.com
Anil Kumar Kataria :
Department of
Veterinary Microbiology and Biotechnology, College of Veterinary
and Animal Science, Rajasthan University of Veterinary and
Animals Sciences, Bikaner, Rajasthan, India; akkataria1@rediffmail.com
Received: 06-01-2016, Accepted: 18-03-2016, Published online:
28-04-2016
Corresponding author:
Taruna Bhati, e-mail: vetcvas.bhati@gmail.com
Citation:
Bhati T, Nathawat P, Sharma SK, Yadav R, Bishnoi J, Kataria AK
(2016) Polymorphism in spa gene of Staphylococcus
aureus from bovine subclinical mastitis, Veterinary
World, 9(4): 421-424.
Abstract
Aim:
The
virulence-associated protein-A of Staphylococcus aureus,
encoded by spa gene shows a variation in length in
different strains. In this study, the spa gene variation
in S. aureus strains was studied which were isolated from
subclinical cases of bovine mastitis.
Materials and
Methods:
About 38
isolates of S. aureus were recovered from
Holstein–Friesian (HF) crossbred (n=16) and Rathi cattle (n=22)
with subclinical mastitis as per standard procedures, and these
isolates were subjected to amplification of spa gene
(X-region) by polymerase chain reaction and calculation of
number of tandem repeats were done.
Results:
Of
the 16 isolates from H-F crossbred cattle, all with the
exception of one isolate produced spa amplicon. Seven
isolates produced amplicons of 200 bp, one produced 160 bp, and
other seven produced spa amplicon of 150 bp with
calculated number of 6, 5, and 4 repeats, respectively, whereas
nine different types of amplicons were produced by 22 S.
aureus isolates from Rathi cattle, viz., 280, 250,
240, 200, 190, 180, 170, 150, and 140 bp with 10, 8, 8, 6, 6, 6,
5, 4, and 4 repeats, respectively. One of the isolates from
Rathi cattle produced two spa amplicons (150 and 190 bp).
Conclusion:
A
greater polymorphism was observed in the S. aureus
isolates from Rathi cattle than from H-F crossbreds with
subclinical mastitis.
Keywords:
cattle, polymorphism, protein-A, spa gene,
Staphylococcus aureus, subclinical mastitis.
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