Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916


Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ

Open Access

Research (Published online: 30-04-2016)

16. Comparative study on immunoglobulin Y transfer from breeding hens to egg yolk and progeny chicks in different breeds of poultry - Ritu Agrawal, S. D. Hirpurkar, C. Sannat and Amit Kumar Gupta

Veterinary World, 9(4): 425-431



   doi: 10.14202/vetworld.2016.425-431



Ritu Agrawal: Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Anjora, Durg, Chhattisgarh, India;

S. D. Hirpurkar: Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Anjora, Durg, Chhattisgarh, India;

C. Sannat: Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Anjora, Durg, Chhattisgarh, India;

Amit Kumar Gupta: Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Anjora, Durg, Chhattisgarh, India;


Received: 16-12-2015, Accepted: 19-03-2016, Published online: 30-04-2016


Corresponding author: C. Sannat, e-mail:

Citation: Agrawal R, Hirpurkar SD, Sannat C, Gupta AK (2016) Comparative study on immunoglobulin Y transfer from breeding hens to egg yolk and progeny chicks in different breeds of poultry, Veterinary World, 9(4): 425-431.

Aim: This study was undertaken to compare the immunoglobulin Y (IgY) level and its efficacy in laying hens of four different breeds of poultry (viz.,Vanraja, Gramapriya, BlackRock, and KalingaBrown) and its relative transfer in egg yolk and chick.

Materials and Methods: This study was conducted in 48 apparently healthy laying hens vaccinated with Salmonella inactivated polyvalent vaccine, eggs and progeny chicks; 12 each from four different breeds of poultry,viz.,Vanraja, Gramapriya, BlackRock, and KalingaBrown. The methodology included measurement of egg and yolk weight, total protein and IgY in egg yolk, total serum protein and IgY in breeding hens, and progeny chicks and extent of IgY transfer from hens to yolk then to chicks. Further, Salmonella-specific antibodies in breeding hens, egg yolk and progeny chicks were assessed using O and H antigen by tube agglutination test.

Results: The egg weight differed nonsignificantly (p>0.05) among breeds, however, breed wise significant variation (p<0.01) was reported in yolk weight. The weight of egg yolk significantly affects the total protein and IgY concentration although these levels per unit of volume did not differ. Total protein was significantly higher (p<0.01) in KalingaBrown and Gramapriya as compared to Vanraja and BlackRock. Non-significant (p>0.05) difference among breed was found in total protein of egg yolk and chick. The IgY concentration in hens, egg yolk and chick was found to be in the range of 5.35±0.63- 5.83±0.65, 2.3±0.1-2.6±0.2, and 1.3±0.11-1.7±0.16 mg/ml, respectively which is uniform and independent of total protein concentration at all the three levels. Significant breed variations were not observed in maternal IgY transfer from breeding hens to chicks and were 25.62±1.42-36.06±4.34% of total IgY in parent flock. Moderate to higher rate of seroprevalence with peak titers of 1:640 against Salmonella-specific antibodies was observed in only 41.6% of breeding hens.

Conclusion: No significant difference in the rate of transfer of IgY was observed in four breeds studied (viz.,Vanraja, Gramapriya, BlackRock, and KalingaBrown) and moderate seropositivity was detected for Salmonella-specific antibodies in progeny chicks.

Keywords: breeding hens, chicks, maternal immunoglobulin Y, Salmonella antibody, yolk.

1. Bar-Shira, E.andFriedman, A.(2006) Development and adaptations of innate immunity in the gastrointestinal tract of the newly hatched chick.Dev. Comp.Immunol.,30(10):930-941.
2. Lawrence, E.C., Arnaud-Battandier, F., Grayson, J., Koski, I.R.,Dooley, N.J., Muchmore, A.V. and Blaese, R.M. (1981) Ontogeny of humoral immune function in normal chickens: A comparison of immunoglobulin-secreting cells in bone marrow, spleen, lungs, and intestine. Clin. Exp. Immunol., 43:450-457.
PMid:7026091 PMCid:PMC1537196
3. Warr, G.W., Magor, K.E. and Higgins, D.A. (1995) IgY: Clues to the origins of modern antibodies. Immunol. Today, 16:392-398.
4. Orlans, E. (1967) Fowl antibody VIII. A comparison of natural, primary, and secondary erythrocytes in hen sera; their transmission to yolk and chick. Immunol.,12:27-37.
5. Kovacs-Nolan,J. and Mine,Y. (2012) Egg yolk antibodies for passive immunity. Annu. Rev. Food Sci. Technol., 3: 163-182.
6. Yan, S., Pendrak, M. and Abela-Ridder, B. (2003)An overview of Salmonella typing public health perspectives. Clin. Appl. Immunol. Rev., 4: 189-204.
7. Reinhold, J.G. (1953)In: Reiner, M., editor. Standard Methods of Clinical Biochemistry. Vol. 1.Academic Press, New York & London. p88.
8. Polson,A., Von Wechmar, M.B. and Van Regenmortel, M.V.H. (1980) Isolation of viral IgY antibodies from yolks of immunized hens.Immunol.Commun.,9: 475-493.
9. Mancini, G., Carbonara, A.O. and Heremans, J.F. (1965) Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochem., 2: 235-254.
10. OIE.(2010) Salmonellosis. Terrestrial Manual. p9-10. Last accessed on 04-12-2015.
11. Bell, D.J. (1971) Plasma enzymes. In: Bell, D.J. and Freeman, B.M., editors. Physiology and Biochemistry of the Domestic Fowl. Academic Press Inc., London. p964-965.
12. EL-Sheikh, A.M.H., Abdalla, E.A. and Hanafy, M.M. (2009) Study on productive performance, haematological and immunological parameter in a local strain of chicken as affected by Mannanoligosaccharide under hot climate conditions. Egypt. Poult. Sci., 29(I): 287-305.
13. Malakian, M., Hassanabadi, A.andHeidariniya, A. (2011) Effects of Safflower seed on performance, carcass traits and blood parameters of Broilers. Res. J. Poult. Sci., 4(2): 18-21.
14. Bowes, V.A., Julian, R.J. and Stirtzinger, T. (1989) Comparison of serum biochemical profiles of male broilers with female broilers and White leghorn chickens. Can. J. Vet. Res., 53: 7-11.
PMid:2914228 PMCid:PMC1255504
15. Kaneko, J.J. (1997) In: Serum proteins and the dysproteinemias. Clinical Biochemistry of Domestic Animals. 5th ed. Academic Press, San Diego, London, Boston, New York, Sydney, Tokyo, Toronto. p117-138.
16. Ritchie, B.W., Harrison, J.G. and Harrison, R.L. (1994) Avian Medicine. Winger's Publishing, Inc., Florida.
17. Li, X., Nakano, T.,Sunwoo, H.H.,Paik, B.H., Chae, H.S. and Sim, J.S. (1998) Effects of egg and yolk weights on yolk antibody (IgY) production in laying chickens. Poult. Sci., 77:266-270.
18. Hamal, K.R., Burgess, S.C., Pevzner, I.Y. and Erf, G.F. (2006)Maternal antibody transfer from dams to their egg yolks, egg whites, and chicks in meat lines of chickens. Poult. Sci., 85: 1364-1372.
19. Carlander, D., Wilhelmson, M. and Larsson, A. (2003) Immunoglobulin Y level in egg yolk from three chicken genotypes. Food Agric.Immunol., 15(1): 35-40.
20. Ulmer-Franco, A.M.,Cherian, G.,Quezada, N.,Fasenko, G.M. andMcMullen, L.M. (2012) Hatching egg and newly hatched chick yolk sac total IgY content at 3 broiler breeder flock ages. Poult. Sci.,91(3):758-764.
21. Meenatchisundaram, S. and Michael, A. (2010) Comparison of four different purification methods for isolation of anti Echiscarinatusantivenom antibodies from immunized chicken egg yolk. Iran.J.Biotechnol., 8: 50-55.
22. Sun, H., Chen, S., Xia, C.,Guiyun, X. and Lujiang, Q. (2013) Effect of transportation duration of 1-day-old chicks on postplacement production performances and pododermatitis of broilers up to slaughter age.Poult. Sci., 92(12): 3300-3309.
23. Pauly,D., Chacana, P.A., Calzado, E.G.,Brembs, B. and Schade, R. (2011)IgYtechnology: Extraction of chicken antibodies from egg yolk by polyethylene glycol (PEG) precipitation. J. Vis. Exp., 51:3084.
24. Niranjan, M., Sharma, R.P., Rajkumar, U., Chatterjee, R.N., Reddy, B.L.N. and Battacharya, T.K. (2008) Egg quality traits in chicken varieties developed for backyard poultry farming in India. Livest. Res.Rural Dev., 20(12): 103-105.
25. Haunshi, S., Doley, S. and Kadirvel, G. (2010) Comparative studies on egg, meat and semen qualities of native and improved chicken varieties developed for backyard poultry production. Trop. Anim. Health Prod., 42(5): 1013-1019.
26. Amir, M.P., Rasaee, M.J., Qujeq, D., Asadikaram, G.H.R. and Moqhadam, M.F. (2000) A simple and economical procedure for the purification of immunoglobulin Y from egg yolk by T-Gel chromatography. Iran. J. Allergy Asthma Immunol., 1(2): 53-57.
27. Liou, J.F., Chang, C.W., Tailiu, J.J., Yu, C.K., Lei, H.Y., Chen, L.R. and Tai, C. (2010)Passive protection effect of chicken egg yolk immunoglobulins on enterovirus 71 infected mice. Vaccine, 28: 8189-8196.
28. Abdel-Moneim, A.S. and Abdel-Gawad, M.M.A. (2006) Genetic variations in maternal transfer and immune responsiveness to infectious bursal disease virus. Vet.Microbiol.,114: 16-24.
29. Loeken, M.R. and Roth, R.E. (1983) Analysis of maternal IgG subpopulations which are transported into the chicken oocyte. Immunol., 49: 21-28.
30. Rose, M.E., Orlans, E. and Buttress, N. (1974) Immunoglobulin classes in the hen's egg: their segregation in yolk and white. Eur. J. Immunol., 4: 521-523.
31. Schade, R., Calzado, E.G., Sarmiento, R., Chacana, P.A., Porankiewicz-Asplund, J. and Terzolo, H.R. (2005) Chicken egg yolk antibodies (IgY-technology): A review of progress in production and use in research and human and veterinary medicine. Altern. Lab. Anim., 33:129-154.
32. Smith,A.L. and Beal,R. (2008)The avian enteric immune system in health and disease. In: Davison, F., Kaspers, B.,Schat, K.A., editors. Avian Immunology. Elsevier Ltd., New York.p243-271.
33. Betancor, L., Pereira, M., Martinez, A., Giossa, G., Fookes, M., Flores, M., Barrios, P., Repiso, V., Vignoli, R., Cordeiro, N., Algorta, G., Thomson, N., Maskell, D., Schelotto, F. and Chabalgoity, J.A. (2010) Prevalence of Salmonella enterica in poultry and eggs in Uruguay during an epidemic due to SalmonellaentericaSerovarenteritidis. J. Clin.Microbiol., 48(7): 2413-2423.
PMid:20484605 PMCid:PMC2897505
34. Kumar, S., Sadana, J.R., Chaturvedi, G.C. and Michra, S.K. (2001) Studies on humoral and cellular immune response in Japanese quail (Coturnixcoturnix japonica) experimentally infected with S. typhimurium. Indian J.Poult. Sci., 36(2): 197-200.
35. Ahmed, A.K.M., Islam, M.T., Haider, M.G. and Hossain, M.M. (2008)Seroprevalence and pathology of naturally infected salmonellosis in poultry with isolation and identification of causal agents. J. Bangladesh Agrilc. Univ., 6(2): 327-334.
36. Hermans, D.,Van Steendam, K.,Verbrugghe, E.,Verlinden, M.,Martel, A.,Seliwiorstow, T.,Heyndrickx, M.,Haesebrouck, F.,De Zutter, L.,Deforce, D. andPasmans, F. (2014) Passive immunization to reduce Campylobacter jejuni colonization and transmission in broiler chickens.Vet. Res.,45:27.
37. Gómez-Verduzco, G., Tellez, G., Quintana, A.L., Isibasi,A.and Ortiz-Navarrete, V. (2010)Humoral immune response in breeding hens and protective immunity provided by administration of purified Salmonella Gallinarumporins. Poult. Sci., 89:495-500.
38. Machado Leal Ribeiro, A., Rudnik, L.,Wageck Canal, C.,RibeiroKratz, L. and Farias,C. (2005) Supply of hipperimmunized hen yolks against swineEscherichia coli to control newborn piglets diarrhea. R. Bras. Zootec., 34:1234-1239.
39. Ulmer-Franco, A.M. (2012)Transfer of chicken immunoglobulin Y (IgY) from the hen to the chick.Avian Biol. Res., 5(2): 81-87.