| 
              
              
              Open Access  
 
              
              
              
              Research 
              
              
(Published 
				online: 07-04-2016)  
              4. 
				
				
				Neonatal mortality in dogs: Prognostic 
				value of Doppler ductus venosus waveform evaluation - 
				Preliminary results - 
				
				Gabriele Barella, Stefano Faverzani, Massimo Faustini, Debora 
				Groppetti and Alessandro Pecile 
              
              Veterinary World, 9(4): 356-360   
              
   
                
                
doi: 
              
				
				10.14202/vetworld.2016.356-360 
                
				  
				
				Gabriele Barella: 
				
				Department of Veterinary Medicine, State University of Milan, 
				Via Celoria 10; 20133; Milan, Italy; gabriele.barella@gmail.com 
				
				Stefano Faverzani: 
				
				Department of Veterinary Medicine, State University of Milan, 
				Via Celoria 10; 20133; Milan, Italy; stefano.faverzani@unimi.it 
				
				Massimo Faustini: 
				
				Department of Veterinary Medicine, State University of Milan, 
				Via Celoria 10; 20133; Milan, Italy; massimo.faustini@unimi.it 
				
				Debora Groppetti: 
				
				Department of Veterinary Medicine, State University of Milan, 
				Via Celoria 10; 20133; Milan, Italy; debora.groppetti@unimi.it 
				
				Alessandro Pecile: 
				
				Department of Veterinary Medicine, State University of Milan, 
				Via Celoria 10; 20133; Milan, Italy; alessandro.pecile@unimi.it   
				
				Received: 25-11-2015, Accepted: 04-03-2016, Published online: 
				07-04-2016 
				  
				
              	
              	Corresponding author:Gabriele Barella, e-mail: gabriele.barella@gmail.com 
 
              Citation: 
				
				Barella G, Faverzani S, Faustini M, Groppetti D, Pecile A (2016) 
				Neonatal mortality in dogs: Prognostic value of Doppler ductus 
				venosus waveform evaluation - Preliminary results, 
				
				Veterinary World, 9(4): 
				356-360. 
 
              
				Abstract 
 
				
				
				Aim: 
				
				To define the prognostic value of Doppler ultrasonographic 
				morphology of ductus venosus (DV) waveform on canine neonatal 
				mortality. 
				
				
				Materials and Methods: 
				
				Fifty-four healthy pregnant bitches underwent fetal 
				ultrasonographic assessment. The DV waveforms were classified as 
				diphasic (dDVw) or triphasic (tDVw) and compared with neonatal 
				mortality. 
				
				
				Results: 
				
				Ninety-three fetuses were evaluated. Twenty fetuses belonged to 
				litters with neonatal mortality, in which tDVw was observed. 
				Seven fetuses belonged to litters without neonatal mortality, in 
				which tDVw was observed. Fifty-eight fetuses belonged to litters 
				without neonatal mortality, in which only dDVw was observed. 
				Eight fetuses belonged to litters with neonatal mortality, in 
				which only dDVw was observed. The correlation between tDVw and 
				neonatal mortality was statistically significant (odds ratio 
				[OR], 20.7; p<0.0001). Considering only pregnancies with one or 
				two fetuses with the same DV waveform: Two fetuses with tDVw 
				belonged to litters with neonatal mortality; 1 foetus with tDVw 
				belonged to litter without neonatal mortality and 26 fetuses 
				showed dDVw without neonatal mortality. The correlation between 
				tDVw and neonatal mortality even in litters up to two pups was 
				statistically significant (OR, 88.3; p=0.01). 
				
				
				Conclusion: 
				
				Echo-Doppler assessment of DV is feasible in canine fetuses, and 
				the presence tDVw seems to be related to neonatal mortality. 
				
				Keywords: 
				
				dog, Doppler, ductus venosus, neonatal mortality, ultrasound. 
 
              References 
 
				
					| 1. Kiresud, T., Eik-Nes, S.H., Blaas, H.G. and Hellevik, L.R. 
					(1992a) Foramen ovale: An ultrasonographic study of its 
					relation to the inferior vena cava, ductus venosus and 
					hepatic veins. Ultrasound Obstet. Gynecol., 2: 389-396. http://dx.doi.org/10.1046/j.1469-0705.1992.02060389.x
 PMid:12796912
 |  
					|  |  
					| 2. Kiresud, T., Eik-Nes, S.H., Blaas, H.G., Hellevik, L.R. 
					and Simensen, B. (1992b) Ductus venosus. A longitudinal 
					Doppler velocimetric study of the human fetus. J. Mater. 
					Fetal Invest., 11: 2-5. |  
					|  |  
					| 3. Panarace, M., Garnil, C., Cané, L., Rodriguez, E. and 
					Medina, M. (2008) Echo-Doppler assessment of resistance and 
					velocity of blood flow in the ductus venosus throughout 
					gestation in fetal lambs. Theriogenology, 70: 648-654. http://dx.doi.org/10.1016/j.theriogenology.2008.04.027
 PMid:18508117
 |  
					|  |  
					| 4. Tchirikov, M., Eisermann, K., Rybakowski, C. and Schroder, 
					H.J. (1998) Doppler ultrasound evaluation of ductus venosus 
					blood flow during acute hypoxemia in fetal lambs. Ultrasound 
					Obstet. Gynecol., 11: 426-431. http://dx.doi.org/10.1046/j.1469-0705.1998.11060426.x
 PMid:9674090
 |  
					|  |  
					| 5. Barella, G., Faverzani, S., Faustini, M., Groppetti, D. 
					and Pecile, A. (2014) Doppler ultrasonographic evaluation of 
					ductus venosus blood flow in 55 canine fetuses. J. 
					Ultrasound, 17: 287-292. http://dx.doi.org/10.1007/s40477-014-0117-2
 PMid:25368686 PMCid:PMC4209219
 |  
					|  |  
					| 6. Vade, A., Lim-Dunham, J. and Iqbal, N. (2001) Imaging of 
					the ductus venosus in neonates. J. Ultrasound Med., 20: 
					681-687. PMid:11400942
 |  
					|  |  
					| 7. Turan, O.M., Turan, S., Berg, C., Gembruch, U., 
					Nicolaides, K.H., Harman, C.R. and Baschat, A.A. (2011) 
					Duration of persistent abnormal ductus venosus flow and its 
					impact of perinatal outcome in fetal growth restriction. 
					Ultrasound Obstet. Gynecol., 8: 295-302. http://dx.doi.org/10.1002/uog.9011
 PMid:21465604
 |  
					|  |  
					| 8. Concannon, P., Hansel, W. and McEntee, K. (1977) Changes 
					in LH, progesterone and sexual behavior associated with 
					preovulatory luteinisation in the bitch. Biol. Reprod., 7: 
					604-613. http://dx.doi.org/10.1095/biolreprod17.4.604
 |  
					|  |  
					| 9. Michel, E., Spörri, M., Ohlerth, S. and Reichler, I. 
					(2011) Prediction of parturition date in the bitch and 
					queen. Reprod. Domest. Anim., 46: 926-939. http://dx.doi.org/10.1111/j.1439-0531.2011.01763.x
 PMid:21366718
 |  
					|  |  
					| 10. Brugger, N., Otzdorff, C., Walter, B., Hoffmann, B. and 
					Braun, J. (2011) Quantitative determination of progesterone 
					(P4) in canine blood serum using an enzyme-lined 
					fluorescence assay. Reprod. Domest. Anim., 46: 870-873. http://dx.doi.org/10.1111/j.1439-0531.2011.01757.x
 PMid:21323757
 |  
					|  |  
					| 11. Groppetti, D., Vegetti, F., Bronzo, V. and Pecile, A. 
					(2015) Breed-specific fetal biometry and factors affecting 
					the prediction of whelping date in the German shepherd dog. 
					Anim. Reprod. Sci., 152: 117-122. http://dx.doi.org/10.1016/j.anireprosci.2014.11.018
 PMid:25510562
 |  
					|  |  
					| 12. Kutzler, M.A., Yeager, A.E., Mohammed, H.O. and Meyers-Wallen, 
					V.N. (2003) Accuracy of canine parturition date prediction 
					using fetal measurements obtained by ultrasonography. 
					Theriogenology, 60: 1309-1317. http://dx.doi.org/10.1016/S0093-691X(03)00146-8
 |  
					|  |  
					| 13. Zone, M.A. and Wanke, M.M. (2011) Diagnosis of canine 
					fetal health by ultrasonography. Reproduction, 57: 215-219. |  
					|  |  
					| 14. Yeager, A.E. and Concannon, P.W. (1990) Association 
					between the preovulatory luteinizing hormone surge and the 
					early ultrasonographic detection of pregnancy and fetal 
					heartbeats in beagle dogs. Theriogenology, 34: 655-665. http://dx.doi.org/10.1016/0093-691X(90)90021-K
 |  
					|  |  
					| 15. Martins, W.P. and Kiresud, T. (2013) How to record 
					ductus venosus blood velocity in the second half of 
					pregnancy. Ultrasound Obstet. Gynecol., 42: 245-245. http://dx.doi.org/10.1002/uog.11082
 PMid:23658000
 |  
					|  |  
					| 16. Maiz, N., Kagan, K.O., Milovanovic, Z., Celik, E. and 
					Nicolaides, K.H. (2008) Learning curve for Doppler 
					assessment of ductus venosus flow at 11 + 0 to 13 + 6 weeks' 
					gestation. Ultrasound Obstet. Gynecol., 31: 503-506. http://dx.doi.org/10.1002/uog.5282
 PMid:18330869
 |  
					|  |  
					| 17. Blanco, P.G., Rodriguez, R., Olguin, S., Rubea, A., 
					Tortora, M. and Gobello, C. (2014) Doppler ultrasonographic 
					assessment of maternal and fetal arteries during normal 
					feline gestation. Anim. Reprod. Sci., 146: 63-69. http://dx.doi.org/10.1016/j.anireprosci.2014.02.003
 PMid:24602508
 |  
					|  |  
					| 18. Di Salvo, P., Bocci, F., Zelli, R. and Polisca, A. 
					(2006) Doppler evaluation of maternal and fetal vessels 
					during normal gestation in the bitch. Res. Vet. Sci., 81: 
					382-388. http://dx.doi.org/10.1016/j.rvsc.2006.03.004
 PMid:16697431
 |  
					|  |  
					| 19. Scotti, L., Di Salvo, P., Bocci, F., Pieramati, C. and 
					Polisca, A. (2008) Doppler evaluation of maternal and foetal 
					vessels during normal gestation in queen. Theriogenology, 
					69: 1111-1119. http://dx.doi.org/10.1016/j.theriogenology.2008.01.025
 PMid:18407345
 |  
					|  |  
					| 20. Gallarreta, F.M.P., Martins, W.P., Nastri, C.O., Filho, 
					F.M., Nicolau, L.G.C., Barra, D.A., Morais, E.N. and Crott, 
					G.C. (2011) Evaluation of ductus venosus and inferior vena 
					cava by using multiple Doppler ultrasound parameters in 
					healthy fetuses. Arch. Gynecol. Obstet., 283: 959-963. http://dx.doi.org/10.1007/s00404-010-1492-3
 PMid:20443013
 |  
					|  |  
					| 21. Gilani, S.A., Javaid, A. and Bala, A.A. (2010) Fetal 
					Doppler ultrasound assessment of ductus venosus in a 20-40 
					weeks gestation normal fetus in the Pakistani population. 
					Med. Ultrasound., 12: 110-113. |  
					|  |  
					| 22. Merz, W.M., Kubler, K., Fimmers, R., Willruth, A., 
					Stoffel-Wagner, B. and Gembruch, U. (2013) Cardiorenal 
					syndrome is present in human fetuses with severe, isolated 
					urinary tract malformations. PLoS One, 8(5): 63664. http://dx.doi.org/10.1371/journal.pone.0063664
 |  |