Open Access
Research
(Published
online: 11-04-2016)
6.
Diagnosis and prevalence of ovine
pulmonary adenocarcinoma in lung tissues of naturally infected
farm sheep -
Ganesh G. Sonawane, Bhupendra Nath Tripathi, Rajiv Kumar and
Jyoti Kumar
Veterinary World, 9(4): 365-370
doi:
10.14202/vetworld.2016.365-370
Ganesh G. Sonawane:
Animal Health Division, ICAR-Central Sheep and Wool Research
Institute, Avikanagar, Malpura, Tonk, Rajasthan, India;
sganesh413@gmail.com
Bhupendra Nath Tripathi:
ICAR-National Research Centre on Equines, Hisar, Haryana, India;
bntripathi1@yahoo.co.in
Rajiv Kumar:
Animal Biotechnology Section, ICAR-Central Sheep and Wool
Research Institute, Avikanagar, Malpura, Tonk, Rajasthan, India;
rajivbiotech028@gmail.com
Jyoti Kumar:
Animal Health Division, ICAR-Central Sheep and Wool Research
Institute, Avikanagar, Malpura, Tonk, Rajasthan, India; jyotivet@gmail.com
Received: 05-10-2015, Accepted: 03-03-2016, Published online:
11-04-2016
Corresponding author:
Ganesh G. Sonawane, e-mail: sganesh413@gmail.com
Citation:
Sonawane GG, Tripathi BN, Kumar R, Kumar J (2016) Diagnosis and
prevalence of ovine pulmonary adenocarcinoma in lung tissues of
naturally infected farm sheep,
Veterinary World, 9(4):
365-370.
Abstract
Aim:
This study was aimed to detect ovine pulmonary adenocarcinoma (OPA)
in sheep flocks affected with pulmonary disorders at organized
farm.
Materials and Methods:
A total of 75 sheep died naturally were thoroughly examined for
the lesions of OPA during necropsy. Tissue sections from
affected portion of the lungs from each animal were collected
aseptically and divided into two parts; one each for polymerase
chain reaction (PCR) and another for histopathology.
Results:
On PCR examination of lung tissues, six sheep (8%) were found to
be positive for JSRV. Two of them were 3-6 months of age and did
not show clinical signs/gross lesions of OPA. Four adult sheep
positive on PCR revealed characteristic lesions of OPA on gross
and histopathological examination.
Conclusion:
In the absence of known specific antibody response to the
infection with JSRV, there is no diagnostic serological test
available. The PCR assay employed in this study on lung tissues,
using primers based on the U3 region of the viral long terminal
repeat for JSRV would be helpful in the screening of preclinical
and clinical cases of OPA in sheep.
Keywords:
diagnosis, lung tissues, ovine pulmonary adenomatosis, sheep.
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