Open Access
Research
(Published
online: 15-04-2016)
9.
Detection of
Mycobacterium tuberculosis
and
Mycobacterium bovis
in Sahiwal cattle from an organized farm
using ante-mortem techniques -
Gursimran Filia, Geeta Devi Leishangthem, Vishal Mahajan and
Amarjit Singh
Veterinary World, 9(4): 383-387
doi:
10.14202/vetworld.2016.383-387
Gursimran Filia:
Animal Disease Research Centre, Guru Angad Dev Veterinary and
Animal Sciences University Ludhiana, Punjab, India; harpalfilia@rediffmail.com
Geeta Devi Leishangthem:
Animal Disease Research Centre, Guru Angad Dev Veterinary and
Animal Sciences University Ludhiana, Punjab, India; drgeetapatho@gmail.com
Vishal Mahajan:
Animal Disease Research Centre, Guru Angad Dev Veterinary and
Animal Sciences University Ludhiana, Punjab, India; mahajanv17@gmail.com
Amarjit Singh:
Animal Disease Research Centre, Guru Angad Dev Veterinary and
Animal Sciences University Ludhiana, Punjab, India;
amarjitsingh64@gmail.com
Received: 11-10-2015, Accepted: 10-03-2016, Published online:
15-04-2016
Corresponding author:
Gursimran Filia, e-mail: harpalfilia@rediffmail.com
Citation:
Filia G, Leishangthem GD, Mahajan V, Singh A (2016) Detection of
Mycobacterium tuberculosis
and
Mycobacterium bovis
in Sahiwal cattle from an organized farm using antemortem
techniques,
Veterinary World, 9(4):
383-387.
Abstract
Aim:
The aim of this study was to investigate the prevalence of
bovine tuberculosis (TB) and detection of
Mycobacterium bovis
in cattle from an organized dairy farm.
Materials and Methods:
A total of 121 animals (93 females and 28 males) of 1 year and
above were studied for the prevalence of bovine TB using single
intradermal comparative cervical tuberculin (SICCT) test, bovine
gamma-interferon (γ-IFN) enzyme immunoassay, and polymerase
chain reactions (PCRs).
Results:
Out of total 121 animals, 17 (14.04%) animals were positive
reactors to SICCT test while only one (0.82%) animal for γ-IFN
assay. By PCR,
Mycobacterium
TB complex was detected in 19 (15.70%) animals out of which 4
(3.30%) animal were also positive for
M. bovis.
Conclusions:
Diagnosis of bovine TB can be done in early stage in live
animals with multiple approaches like skin test followed by a
molecular technique like PCR which showed promising results.
Keywords:
bovine gamma interferon assay, bovine tuberculosis,
Mycobacterium bovis,
Mycobacterium tuberculosis
complex, polymerase chain reaction, single intradermal
comparative cervical tuberculin test.
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