Veterinary World

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Research (Published online: 15-04-2016)

9. Detection of Mycobacterium tuberculosis and Mycobacterium bovis in Sahiwal cattle from an organized farm using ante-mortem techniques - Gursimran Filia, Geeta Devi Leishangthem, Vishal Mahajan and Amarjit Singh

Veterinary World, 9(4): 383-387



   doi: 10.14202/vetworld.2016.383-387



Gursimran Filia: Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab, India;

Geeta Devi Leishangthem: Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab, India;

Vishal Mahajan: Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab, India;

Amarjit Singh: Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab, India;


Received: 11-10-2015, Accepted: 10-03-2016, Published online: 15-04-2016


Corresponding author: Gursimran Filia, e-mail:

Citation: Filia G, Leishangthem GD, Mahajan V, Singh A (2016) Detection of Mycobacterium tuberculosis and Mycobacterium bovis in Sahiwal cattle from an organized farm using antemortem techniques, Veterinary World, 9(4): 383-387.

Aim: The aim of this study was to investigate the prevalence of bovine tuberculosis (TB) and detection of Mycobacterium bovis in cattle from an organized dairy farm.

Materials and Methods: A total of 121 animals (93 females and 28 males) of 1 year and above were studied for the prevalence of bovine TB using single intradermal comparative cervical tuberculin (SICCT) test, bovine gamma-interferon (γ-IFN) enzyme immunoassay, and polymerase chain reactions (PCRs).

Results: Out of total 121 animals, 17 (14.04%) animals were positive reactors to SICCT test while only one (0.82%) animal for γ-IFN assay. By PCR, Mycobacterium TB complex was detected in 19 (15.70%) animals out of which 4 (3.30%) animal were also positive for M. bovis.

Conclusions: Diagnosis of bovine TB can be done in early stage in live animals with multiple approaches like skin test followed by a molecular technique like PCR which showed promising results.

Keywords: bovine gamma interferon assay, bovine tuberculosis, Mycobacterium bovis, Mycobacterium tuberculosis complex, polymerase chain reaction, single intradermal comparative cervical tuberculin test.

1. Ayele, W.Y., Neil, S.D., Zinsstag, J., Weiss, M.G. and Pavlik, I. (2004) Bovine tuberculosis: An old disease but a new threat to Africa. Int. J. Tuberculosis Lung Dis., 8(8): 924-937.
2. Grange, J.M. (2001) Mycobacterium bovis infection in human beings. Tuberculosis (Edinb), 81(1-2): 71-77.
3. Thoen, C.O., LoBue, P.A. and de Kantor, I. (2006) The importance of Mycobacterium bovis as a zoonosis. Vet. Microbiol., 112(2-4): 339-345.
4. TB India. (2012) Revised National TB Control Programme, Annual Status Report, New Delhi, India. Available from: http// [Accessed on 20-07-2015].
5. Courtenay, O., Reilly, L.A., Sweeney, F.P., Hibberd, V., Bryan, S., Ul-Hassan, A., Newman, C., Macdonald, D.W., Delahay, R.J., Wilson, G.J. and Wellington, E.M.H. (2006) Is Mycobacterium bovis in the environment important for the persistence of bovine tuberculosis? Biol. Lett., 2(3): 460-462.
PMid:17148430 PMCid:PMC1686208
6. Napp, S., Allepuz, A., Mercader, I., Nofrarı´as, M., Lo´pez-Soria, S., Domingo, M., Romero, B., Bezos, J. and de Val, B.P. (2013) Evidence of goats acting as domestic reservoirs of bovine tuberculosis. Vet. Rec., 172(25): 663.
7. Zanardi, G., Boniotti, M.B., Gaffuri, A., Casto, B., Zanoni, M. and Pacciarini, ML. (2013) Tuberculosis transmission by Mycobacterium bovis in a mixed cattle and goat herd. Res. Vet. Sci., 95(2): 430-433.
8. Okafor, C.C., Grooms, D.L., Bruning-Fann, C.S., Averill, J.J. and Kaneene, J.B. (2011) Descriptive epidemiology of bovine tuberculosis in Michigan (1975-2010): Lessons learned. Vet. Med. Int., 2011: 13.
PMid:21776355 PMCid:PMC3135262
9. OIE. (2009) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. World Organization for Animal Health, Paris, France.
10. Hermans, P.W., Soolingen, D.V., Dale, J.W., Schuitema, A.R.J., Mcadam, R.A., Catty, D. and. Vanembden, J.A. (1990) Insertion element IS986 from Mycobacterium tuberculosis: A useful tool for diagnosis and epidemiology of tuberculosis. J. Clin. Microbiol., 28(9): 2051-2058.
PMid:1977765 PMCid:PMC268102
11. Rodriguez, G.M., Gold, B., Gomez, M., Dussurget, O. and Smith, I. (1999) Identification and characterization of two divergently transcribed iron regulated genes in Mycobacterium tuberculosis. Tuber. Lung. Dis., 79: 287-298.
12. De la Rua-Domenech, R., Goodchild, A.T., Vordermeier, H.M., Hewinson, R.G., Christiansen, K.H. and Clifton-Hadley, R.S. (2006) Ante mortem diagnosis of tuberculosis in cattle: A review of the tuberculin tests, gamma-interferon assay and other ancillary diagnostic techniques. Res. Vet. Sci., 81(2): 190-210.
13. Gormley, E., Doyle, M.B., Fitzsimons, T., Mcgill, K. and Collins, J.D. (2006) Diagnosis of M. bovis infection in cattle by use of gamma-interferon (BovigamŽ) assay. Vet. Microbiol., 112(2-4): 171-179.
14. Pollock, J.M., McNair, J., Welsh, M.D., Girvin, R.M., Kennedy, H.E., Mackie, D.P. and Neill, S.D. (2001) Immune responses in bovine tuberculosis. Tuberculosis (Edinb), 81(1-2): 103-107.
15. Pollock, J.M., Welsh, M.D. and McNair, J. (2005) Immune response in bovine tuberculosis: Towards new strategies for the diagnosis and control of disease. Vet. Immunol. Immunopathol., 108(1-2): 37-43.
16. Praud, A., Boschiroli, M.L., Meyer, L., Garin-Bastuji, B. and Dufour, B. (2015) Assessment of the sensitivity of the gamma-interferon test and the single intradermal comparative cervical test for the diagnosis of bovine tuberculosis under field conditions. Epidemiol. Infect., 143(1): 157-166.
17. Neeraja, D., Veeregowda, B.M., Rani, M.S., Rathnamma, D., Bhaskaran, R. and Leena, G. (2014) Comparison of single intradermal test, gamma interferon assay and indirect ELISA for the diagnosis of tuberculosis in a dairy farm. Asian J. Anim. Vet. Adv., 9(9): 593-598.
18. Pollock, J.M. and Neill, S.D. (2002) Mycobacterium bovis infection and tuberculosis in cattle. Vet. J., 163(2): 115-127.
19. Serrano-Moreno, B.A., Romero, T.A., Arriaga, C., Torres, R.A., Pereira-Suarez, A.L. and Garcia-Salazar, J.A. (2008) High Frequency of Mycobacterium bovis DNA in colostra from tuberculous cattle detected by Nested PCR. Zoonoses Public Health., 55(5): 258-266.
20. Figueiredo, E.E.S., Silvestre, F.G., Campos, W.N., Furlanetto, L.V., Medeiros, L. and Lilenbaum, W. (2010) Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR. Braz. J. Microbiol., 41(2): 386-390.
21. Coetsier, C., Vannuffel, P., Blondeel, N., Denef, J.F., Cocito, C. and Gala, J.L. (2000) Duplex PCR for differential identification of Mycobacterium bovis, M. avium, and M. avium subsp. paratuberculosis in formalin-fixed paraffin-embedded tissues from cattle. J. Clin. Microbiol., 38(8): 3048-3054.
PMid:10921976 PMCid:PMC87183
22. Gomez-Laguna, J., Carrasco, L., Ramis, G., Quereda, J.J., Gomez, S. and Pallares, F.J. (2010) Use of real-time and classic polymerase chain reaction assays for the diagnosis of porcine tuberculosis in formalin-fixed, paraffin-embedded tissues. J. Vet. Diagn. Investig., 22(1): 123-127.
23. Rodriguez, J.G., Fissanoti, J.C., Del Portillo, P., Patarroyo, M.E., Romano, M.I. and Cataldi, A. (1999) Amplification of a 500-base-pair fragment from cultured isolates of Mycobacterium bovis. Eur. J. Clin. Microbiol. Infect. Dis., 37(7): 2330-2332.
24. Shah, D.H., Verma, R., Bakshi, C.S. and Singh, R.K. (2002) A multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. FEMS. Microbiol. Lett., 214: 39-43.
25. Sechi, L.A., Dupre, I., Leori, G., Fadda, G. and Zanetti, S. (2000) Distribution of a specific 500-base-pair fragment in Mycobacterium bovis isolates from Sardinian cattle. J. Clin. Microbiol., 38(10): 3837-3839.
PMid:11015414 PMCid:PMC87487