Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916


Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ

Open Access

Research (Published online: 22-08-2016)

14. Low-level laser therapy: Case-control study in dogs with sterile pyogranulomatous pododermatitis - Roberta Perego, D. Proverbio, A. Zuccaro and E. Spada

Veterinary World, 9(8): 882-887



   doi: 10.14202/vetworld.2016.882-887



Roberta Perego: Department of Veterinary Sciences for Health, Animal Production and Food Safety (VESPA), University of Milan, Via Celoria 10, 20133 Milano, Italy;

D. Proverbio: Department of Veterinary Sciences for Health, Animal Production and Food Safety (VESPA), University of Milan, Via Celoria 10, 20133 Milano, Italy;

A. Zuccaro: Department of Veterinary Sciences for Health, Animal Production and Food Safety (VESPA), University of Milan, Via Celoria 10, 20133 Milano, Italy;

E. Spada: Department of Veterinary Sciences for Health, Animal Production and Food Safety (VESPA), University of Milan, Via Celoria 10, 20133 Milano, Italy;


Received: 28-12-2015, Accepted: 19-07-2016, Published online: 22-08-2016


Corresponding author: Roberta Perego, e-mail:

Citation: Perego R, Proverbio D, Zuccaro A, Spada E (2016) Low-level laser therapy: Case-control study in dogs with sterile pyogranulomatous pododermatitis, Veterinary World, 9(8): 882-887.

Aim: Low-level laser therapy (LLLT) is a therapeutic photobiostimulation with properties in reducing swelling, inflammation, and promoting tissue healing. The objective of this pilot study was to evaluate LLLT in sterile pyogranulomatous pododermatitis in five dogs.

Materials and Methods: In each dog, one lesion was designated as the control (treated with a 0.0584% hydrocortisone aceponate spray), and one or more other lesions were treated with a gallium aluminum arsenide-laser, daily for 5 days. Lesions were scored before treatment (D0), at the end (D4), 16 days after the last laser treatment (D20), and after 2 months (D65).

Results: Comparing the treated lesion group with the control lesion group, the clinical score was similar at D0, whereas there was a statistically significant difference at D4 and D20; in the treated group over time, there was a statistically significant improvement between D0, D4, and D20. Lesion recurrence was absent in more than 50% of the treated lesions at D65. No adverse reactions were reported.

Conclusion: Given the positive results of this first clinical study, it would be interesting to extend the study to confirm the validity of this type of therapy in sterile pyogranulomatous pododermatitis in the dog.

Keywords: case-control study, dog, low-level laser therapy, skin, sterile pyogranulomatous pododermatitis.

1. Avci, P., Gupta, A., Sadasivam, M., Vecchio, D., Pam, Z., Pam, N. and Hamblin, M.R. (2013), Low-level laser (light) therapy (LLLT) in skin: Stimulating, healing, restoring. Semin. Cutan. Med. Surg., 32(1): 41-52.
PMid:24049929 PMCid:PMC4126803
2. Posten, W., Wrone, D.A., Dover, J.S., Arndt, K.A., Silapunt, S. and Alam, M. (2005) Low-level laser therapy for wound healing: Mechanism and efficacy. Dermatol. Surg., 31(3): 334-340.
3. Houreld, N.N., Masha, R.T. and Abrahamse, H. (2012) Low-intensity laser irradiation at 660 nm stimulates cytochrome C oxidase in stressed fibroblast cells. Lasers Surg. Med., 44(5): 429-434.
4. Enwemeka, C.S., Parker, J.C., Dowdy, D.S., Harkness, E.E., Sanford, L.E. and Wood-ruff, L.D. (2004) The efficacy of low-power lasers in tissue repair and pain control: A meta-analysis study. Photomed. Laser Surg., 22(4): 323-329.
5. Kajagar, B.M., Godhi, A.S., Pandit, A. and Khatri, S. (2012) Efficacy of low level la-ser therapy on wound healing in patients with chronic diabetic foot ulcers - A randomised control trial. Indian J. Surg., 74(5): 359-363.
PMid:24082586 PMCid:PMC3477409
6. Barolet, D. and Boucher, A. (2010) Prophylactic low-level light therapy for the treat-ment of hypertrophic scars and keloids: A case series. Lasers Surg. Med., 42(6): 597-601.
7. Woodruff, L.D., Bounkeo, J.M., Brannon, W.M., Dawes, K.S., Barham, C.D., Waddell, D.L. and Enwemeka, C.S. (2004) The efficacy of laser therapy in wound repair: A meta-analysis of the literature. Photomed. Laser Surg., 22(3): 241-247.
8. Chen, C., Hou, W.H., Chan, E.S.Y., Yeh, M.L. and Lo, H.L.D. (2014) Phototherapy for treating pressure ulcers. Cochrane Database Syst. Rev., 7: 1-42.
9. Barreto, J.G. and Salgado, C.G. (2010) Clinic-epidemiological evaluation of ulcers in patients with leprosy sequelae and the effect of low level laser therapy on wound heal-ing: A randomized clinical trial. BMC Infect. Dis., 10: 237.
PMid:20698989 PMCid:PMC2928232
10. Garcia, V.G., de Lima, M.A., Okamoto, T., Milanezi, L.A., Júnior, E.C.G., Fernandes, L.A. and Theodoro, L.H. (2010) Effect of photodynamic therapy on the healing of cu-taneous third-degree-burn: Histological study in rats. Lasers Med. Sci., 25(2): 221-228.
11. Dawood, M.S. and Salman, S.D. (2013) Low level diode laser accelerates wound heal-ing. Lasers Med. Sci., 28(3): 941-945.
12. Schlager, A., Kronberger, P., Petschke, F. and Ulmer, H. (2000) Low-power laser light in the healing of burns: A comparison between two different wavelengths (635 nm and 690 nm) and a placebo group. Lasers Surg. Med., 27(1): 39-42.<39::AID-LSM5>3.0.CO;2-4
13. Petersen, S.L., Botes, C., Olivier, A. and Guthrie, A.J. (1999) The effect of low level laser therapy (LLLT) on wound healing in horses. Equine Vet. J., 31(3): 228-231.
14. Fretz, P.B. and Li, Z. (1992) Low energy laser irradiation treatment for second inten-tion wound healing in horses. Can. Vet. J., 33(10): 650-653.
PMid:17424089 PMCid:PMC1481396
15. Ghamsari, S.M., Taguchi, K., Abe, N., Acorda, J.A., Sato, M. and Yamada, H. (1997) Evaluation of low level laser therapy on primary healing of experimentally induced full thickness teat wounds in dairy cattle. Vet. Surg., 26(2): 114-120.
16. Lucroy, M.D., Edwards, B.F. and Madewell, B.R. (1999) Low-intensity laser light-induced closure of a chronic wound in a dog. Vet. Surg., 28(4): 292-295.
17. Kurach, L.M., Stanley, B.J., Gazzola, K.M., Fritz, M.C., Steficek, B.A., Hauptman, J.G. and Seymour, K.J. (2015) The effect of low-level laser therapy on the healing of open wounds in dogs. Vet. Surg., 44(8): 988-996.
18. Breathnach, R.M., Fanning, S., Mulcahy, G., Bassett, H.F. and Jones, B.R. (2008) Ca-nine pododermatitis and idiopathic disease. Vet. J., 176(2): 146-157.
19. Houston, D.M., Clark, E.G., Matwichuk, C.L. and Teachout, D.J. (1993) A case of cu-taneous sterile pyogranuloma/granuloma syndrome in a golden retriever. Can. Vet. J., 34(2): 121-122.
PMid:17424167 PMCid:PMC1686385
20. Breathnach, R.M., Baker, K.P., Quinn, P.J., McGeady, T.A., Aherne, C.M. and Jones, B.R. (2005) Clinical, immunological and histopathological findings in a subpopulation of dogs with pododermatitis. Vet. Dermatol., 16(6): 364-372.
21. Miller W.H., Griffin, C.E. and Campbell, K.L. (2013) Muller and Kirk's Small Animal Dermatology. 7th ed. St. Luis, Missouri: Elsevier, Mosby.
22. Stotts, N.A., Rodeheaver, G.T., Thomas, D.R., Frantz, R.A., Bartolucci, A.A., Sussman, C. and Maklebust, J. (2001) An instrument to measure healing in pressure ulcers: De-velopment and validation of the pressure ulcer scale for healing (PUSH). J. Gerontol., 56(12): M795-M799.
23. Bates-Jensen, B.M., Guihan, M., Garber, S.L., Chin, A.S. and Burns, S.P. (2009) Char-acteristics of recurrent pressure ulcers in veterans with spinal cord injury. J. Spinal Cord. Med., 32(1): 34-42.
PMid:19264047 PMCid:PMC2647498
24. Falanga, V., Saap, L.J. and Ozonoff, A. (2006) Wound bed score and its correlation with healing of chronic wounds. Dermatol. Ther., 19: 383-390.
25. Stich, A.N., Rosenkrantz, W.S. and Griffin, C.E. (2014) Clinical efficacy of low-level laser therapy on localized canine atopic dermatitis severity score and localized pruritic visual analog score in pedal pruritus due to canine atopic dermatitis. Vet. Dermatol., 25(5): 464-e74.
26. Olivieri, L., Cavina, D., Radicchi, G., Miragliotta, V. and Abramo, F. (2015) Efficacy of low-level laser therapy on hair regrowth in dogs with non-inflammatory alopecia: A pilot study. Vet. Dermatol., 26(1): 35-39, e11.
27. Casalechi, H.L., Leal-Junior, E.C.P., Xavier, M., Silva, J.A., de Carvalho Pde, T.C., Aimbire, F. and Albertini, R. (2013) Low-level laser therapy in experimental model of collagenase-induced tendinitis in rats: Effects in acute and chronic inflammatory phas-es. Lasers Med. Sci., 28(3): 989-995.
28. Boschi, E.S., Leite, C.E., Saciura, V.C., Caberlon, E., Lunardelli, A., Bitencourt, S. and Oliveira, J.R. (2008) Anti-inflammatory effects of low-level laser therapy (660 nm) in the early phase in carrageenan - Induced pleurisy in rat. Lasers Surg. Med., 40: 500-508.
29. Schindl, A., Schindl, M., Schön, H., Knobler, R., Havelec, L. and Schindl, L. (1998) Low-intensity laser irradiation improves skin circulation in patients with diabetic mi-croangiopathy. Diabetes Care, 21(4): 580-584.
30. Whitney, J.C. (1970) Some aspects of inter digital cysts in the dog. J. Small Anim. Pract., 11(2): 83-92.
31. Duclos, D.D., Hargis, A.M. and Hanley, P.W. (n.d.) Pathogenesis of canine interdigital palmar and plantar comedones and follicular cysts, and their response to laser surgery. Vet. Dermatol., 19: 134-141.
32. Karayannopoulou, M., Psalla, D., Kazakos, G., Loukopoulos, P., Giannakas, N., Savvas, I., Kritsepi-Konstantinou, M., Chantes, A. and Papazoglou, L.G. (2015) Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs. Vet. Comp. Orthop. Traumatol., 28(3): 172-178.
33. Kim, J.W., Lee, J.H., Lyoo, Y.S., Jung, D.I. and Park, H.M. (2013) The effects of topi-cal mesenchymal stem cell transplantation in canine experimental cutaneous wounds. Vet. Dermatol., 24(2): 242-e53.
PMid:23432413 PMCid:PMC3618380