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Research (Published online: 30-08-2016)

21. Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction - Raghavendra Prasad Mishra, Udit Jain and Rakesh Kumar Singh

Veterinary World, 9(8): 919-921



   doi: 10.14202/vetworld.2016.919-921



Raghavendra Prasad Mishra: Department of Veterinary Public Health, Veterinary University, UP Pandit Deen Dayal Upadhyaya Veterinary Science University, Mathura, Uttar Pradesh, India;

Udit Jain: Department of Veterinary Public Health, Veterinary University, UP Pandit Deen Dayal Upadhyaya Veterinary Science University, Mathura, Uttar Pradesh, India;

Rakesh Kumar Singh: Veterinary Officer, Kanpur Zoo, Kanpur, Uttar Pradesh, India;


Received: 15-04-2016, Accepted: 22-07-2016, Published online: 30-08-2016


Corresponding author: Raghavendra Prasad Mishra, e-mail:

Citation: Mishra RP, Jain U, Singh RK (2016) Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction, Veterinary World, 9(8): 919-921.

Aim: This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance.

Materials and Methods: A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR).

Results: All fecal samples were positive for E. coli. All the E. coli isolates were screened by PCR to detect virulence genes stx1, stx2, eaeA, and hlyA. Of these, 15 isolates were found positive for VTEC having one or more genes in different combinations.

Conclusion: Genes such as stx1, stx2, eaeA, and hlyA were prevalent in VTEC isolates from feces of deer. The presence of VTEC isolates having virulent genes may pose a threat to public health.

Keywords: deer, polymerase chain reaction, verocytotoxic Escherichia coli.

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