Open Access
Research
(Published
online: 08-08-2016)
5.
Epidemiological and immunopathological studies on
Porcine parvovirus
infection in Punjab -
Amninder Kaur, V. Mahajan, G. D. Leishangthem, N. D. Singh,
Payal Bhat, H. S. Banga and G. Filia
Veterinary World, 9(8): 827-831
doi:
10.14202/vetworld.2016.827-831
Amninder Kaur:
Department of Veterinary Pathology, Guru Angad Dev Veterinary
and Animal Sciences University, Ludhiana - 141 004, Punjab,
India; dramninder90@gmail.com
V. Mahajan:
Animal Disease Research Centre, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana - 141 004, Punjab, India;
mahajanv17@gmail.com
G. D. Leishangthem:
Animal Disease Research Centre, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana - 141 004, Punjab, India;
drgeetapatho@gmail.com
N. D. Singh:
Department of Veterinary Pathology, Guru Angad Dev Veterinary
and Animal Sciences University, Ludhiana - 141 004, Punjab,
India; drndsingh@gmail.com
Payal Bhat:
Department of Veterinary Pathology, Guru Angad Dev Veterinary
and Animal Sciences University, Ludhiana - 141 004, Punjab,
India; dr.payalbhat@gmail.com
H. S. Banga:
Department of Veterinary Pathology, Guru Angad Dev Veterinary
and Animal Sciences University, Ludhiana - 141 004, Punjab,
India; bangahs3@yahoo.com
G. Filia:
Animal Disease Research Centre, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana - 141 004, Punjab, India;
harpalfilia@rediffmail.com
Received: 10-03-2016, Accepted: 30-06-2016, Published online:
08-08-2016
Corresponding author:
V. Mahajan, e-mail: mahajanv17@gmail.com
Citation:
Kaur A, Mahajan V, Leishangthem GD, Singh ND, Bhat P, Banga HS,
Filia G (2016) Epidemiological and immunopathological studies on
Porcine parvovirus
infection in Punjab,
Veterinary World, 9(8):
827-831.
Abstract
Aim:
The aim of this study was to get the first-hand knowledge about
the seroprevalence of
Porcine parvovirus
(PPV) in Punjab and a diagnosis of PPV from abortion cases of
swine using gross, histopathological, and
immunohistopathological techniques to observe the tissue tropism
of the virus strain.
Materials and Methods:
Tissue samples from the reproductive tract of pig (n=32),
placental tissue (n=10), and aborted fetuses (n=18) were
collected from Postmortem Hall of the Department of Veterinary
Pathology, GADVASU, field outbreaks and from butcher houses in
and around Ludhiana. These samples were processed for
histopathological and immunohistochemical (IHC) studies. For
seroprevalence study, 90 serum samples of different sex and age
were collected from 15 swine farms of Punjab and were subjected
to indirect enzyme linked immunosorbent assay using commercial
kit.
Results:
Overall, seroprevalence of PPV was found to be 41.1%. Sex and
age related difference in the prevalence was noted. In abortion
cases grossly congested and emphysematous lungs, congested
internal organs with fluid in abdominal cavity and congestion in
brain, changes were noted in fetuses, while diffuse hemorrhages
and edema was observed in placental tissue. Histopathologically,
the most frequent fetal lesions in aborted fetuses were noted in
lungs, liver, and brain. IHC staining revealed PPV antigens in
sections of heart, liver, lung, spleen, brain, lymph node of
fetuses, placenta, and uterus of sow. Gross, histopathological,
and IHC examination of the samples confirmed 5 fetus, 2 placenta
and 3 female reproductive samples positive for parvovirus
infection.
Conclusions:
Seroprevalence results may serve as a support either in
prevention or control of the disease. IHC is the sensitive
technique for diagnosis of PPV associated with the reproductive
tract of swine and was found to supplement the gross and
histopathological alterations, respectively, associated with the
disease.
Keywords:
abortion, enzyme-linked immunosorbent assay, histopathology,
immunohistochemistry, indirect,
Porcine parvovirus,
swine.
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