Open Access
Research
(Published
online: 13-08-2016)
9.
Toxoplasma
infection in sheep from south of Iran
monitored by serological and molecular methods; risk assessment
to meat consumers -
Belal Armand, Kavous Solhjoo, Manoochehr Shabani-Kordshooli,
Mohammad Hasan Davami and Mehdi Sadeghi
Veterinary World, 9(8): 850-855
doi:
10.14202/vetworld.2016.850-855
Belal Armand:
Department of Parasitology and Mycology, Jahrom University of
Medical Sciences, Jahrom, Iran; armand_pl91@yahoo.com
Kavous Solhjoo:
Department of Parasitology and Mycology, Jahrom University of
Medical Sciences, Jahrom, Iran; Department of Parasitic Disease,
Zoonoses Research Center, Jahrom University of Medical Sciences,
Jahrom, Iran; solhjouk@yahoo.com
Manoochehr Shabani-Kordshooli:
Department of Parasitic Disease, Zoonoses Research Center,
Jahrom University of Medical Sciences, Jahrom, Iran; shabani.bi@gmail.com
Mohammad Hasan Davami:
Department of Parasitology and Mycology, Jahrom University of
Medical Sciences, Jahrom, Iran; davamimh@gmail.com
Mehdi Sadeghi:
Department of Biology, Islamic Azad University, Shiraz Branch,
Shiraz, Iran; sadeghi.mehdi419@gmail.com
Received: 18-05-2016, Accepted: 11-07-2016, Published online:
13-08-2016
Corresponding author:
Kavous Solhjoo, e-mail: solhjouk@yahoo.com
Citation:
Armand B, Solhjoo K, Shabani-Kordshooli M, Davami MH, Sadeghi M
(2016)
Toxoplasma
infection in sheep from south of Iran monitored by serological
and molecular methods; risk assessment to meat consumers,
Veterinary World, 9(8):
850-855.
Abstract
Aim:
Toxoplasma gondii
has a clinical and veterinary importance as it is known to cause
congenital disease and abortion both in humans and livestock.
Since the contaminated lamb is one of the sources of human
infection, this study was performed to determine the prevalence
of
T. gondii
in sheep in south of Iran.
Materials and Methods:
Sera and tissue samples (diaphragm and heart) were collected
from 370 sheep from slaughterhouse of Jahrom. The samples were
taken from both sexes and from 6 to 60 months age. Specific
immunoglobulin G antibodies to
T. gondii
were examined with enzyme-linked immunosorbent assay, and
B1
gene nested-polymerase chain reaction detection was done to
survey the tissue samples.
Results:
The total prevalence of
Toxoplasma
infection among sheep was found to be 35.94% and 34.32% based on
serological and molecular method, respectively. According to
serologic and molecular findings, the females were more positive
than males for
Toxoplasma;
maximum frequency of positive samples was observed in 24-36
months and the positive samples had been collected more in
spring than in summer, but no statistical correlation was
observed between prevalence rate and the age and sex of animals
or season of sampling.
Conclusion:
T. gondii
is widely distributed in sheep in Jahrom with a rate comparable
with other parts of Iran and the world. It suggested a
widespread exposure of sheep in this region to
T. gondii.
Thus, consumption of undercooked or raw meat presents the
transmission risk of the parasite and this might be considered
as an important public health problem, mainly for high-risk
groups such as the pregnant and the immunodeficient.
Keywords:
B1
gene, enzyme-linked immunosorbent assay, meat consumers,
nested-polymerase chain reaction, sheep,
Toxoplasma gondii.
References
|
1. Bowman, D.D., Hendrix, C.M., Lindsay, D.S. and Barr, S.C.
(2008) Feline Clinical Parasitology. John Wiley & Sons,
Chichester, UK. |
|
|
|
2. Webster, J.P. (2010) Review of Toxoplasmosis of Animals
and Humans. (Second Edition). by Dubey, J.P. Parasit.
Vectors., 2010;3:112. |
|
|
3. Bossi, P. and Bricaire, F. (2004) Severe acute
disseminated toxoplasmosis. Lancet, 364(9434): 579.
http://dx.doi.org/10.1016/s0140-6736(04)16841-4 |
|
|
4. Pereira-Chioccola, V.L., Vidal, J.E. and Su, C. (2009)
Toxoplasma gondii infection and cerebral toxoplasmosis in
HIV-infected patients. Future Microbiol., 4(10): 1363-1379.
http://dx.doi.org/10.2217/fmb.09.89
PMid:19995194 |
|
|
5. Lundén, A. and Uggla, A. (1992) Infectivity of Toxoplasma
gondii in mutton following curing, smoking, freezing or
microwave cooking. Int. J. Food Microbiol., 15(3): 357-363.
http://dx.doi.org/10.1016/0168-1605(92)90069-F |
|
|
6. Dubey, J.P. (2009) Toxoplasmosis in sheep-the last 20
years. Vet. Parasitol., 163(1): 1-14.
http://dx.doi.org/10.1016/j.vetpar.2009.02.026
PMid:19395175 |
|
|
7. Matsuo, K., Kamai, R., Uetsu, H., Goto, H., Takashima, Y.
and Nagamune, K. (2014) Seroprevalence of Toxoplasma gondii
infection in cattle, horses, pigs and chickens in Japan.
Parasitol. Int., 63(4): 638-639.
http://dx.doi.org/10.1016/j.parint.2014.04.003
PMid:24780140 |
|
|
8. Hill, D.E. and Dubey, J.P. (2013) Toxoplasma gondii
prevalence in farm animals in the United States. Int. J.
Parasitol., 43(2): 107-113.
http://dx.doi.org/10.1016/j.ijpara.2012.09.012
PMid:23201235 |
|
|
9. Dehkordi, F.S., Haghighi Borujeni, M.R., Rahimi, E. and
Abdizadeh, R. (2013) Detection of Toxoplasma gondii in raw
caprine, ovine, buffalo, bovine, and camel milk using cell
cultivation, cat bioassay, capture ELISA, and PCR methods in
Iran. Pathog. Dis., 10(2): 120-125.
http://dx.doi.org/10.1089/fpd.2012.1311
PMid:23441913 |
|
|
10. Asgari, Q., Sarnevesht, J., Kalantari, M., Sadat, S.J.A.,
Motazedian, M.H. and Sarkari, B. (2011) Molecular survey of
toxoplasma infection in sheep and goat from Fars province,
Southern Iran. Trop. Anim. Health Prod., 43(2): 389-392.
http://dx.doi.org/10.1007/s11250-010-9704-1
PMid:20936348 |
|
|
|
11. Habibi, G.R., Imani, A.R., Gholami, M.R., Hablolvarid,
M.H., Behroozikhah, A.M., Lotfi, M. and Bozorgi, S. (2012)
Detection and identification of Toxoplasma gondii type one
infection in sheep aborted fetuses in Qazvin Province of
Iran. J. Parasitol., 7(3): 64-72. |
|
|
12. Sharif, M., Daryani, A., Ebrahimnejad, Z., Gholami, S.,
Ahmadpour, E., Borhani, S. and Lamsechi, N. (2016)
Seroprevalence of anti-toxoplasma IgG and IgM among
individuals who were referred to medical laboratories in
Mazandaran Province, Northern Iran. J. Infect. Public
Health, 9(1): 75-80.
http://dx.doi.org/10.1016/j.jiph.2015.06.006
PMid:26159578 |
|
|
13. Navidpour, S. and Hoghooghi-Rad, N. (1998)
Seroprevalence of anti - Toxoplasma gondii antibodies in
buffaloes in Khoozestan Province, Iran. Vet. Parasitol.,
77(2): 191-194.
http://dx.doi.org/10.1016/S0304-4017(97)00148-9 |
|
|
14. Sharif, M., Gholami, S.H., Ziaei, H., Daryani, A.,
Laktarashi, B., Ziapour, S.P. and Vahedi, M. (2007)
Seroprevalence of Toxoplasma gondii in cattle, sheep and
goats slaughtered for food in Mazandaran Province, Iran,
during 2005. Vet. J., 174(2): 422-424.
http://dx.doi.org/10.1016/j.tvjl.2006.07.004
PMid:16919980 |
|
|
15. Ghazaei, C. (2006) Serological survey of antibodies to
Toxoplasma gondii. Afr. J. Health Sci., 12(3): 114-117.
http://dx.doi.org/10.4314/ajhs.v12i3.30808 |
|
|
|
16. Assmar, M., Amirkhani, A., Piazak, N., Hovanesian, A.,
Kooloobandi, A. and Etessami, R. (1996) Toxoplasmosis in
Iran. Results of a seroepidemiological study. Bull. Soc.
Pathol. Exot., 90(1): 19-21. |
|
|
|
17. Asgari, Q., Moazzeni, M., Mohajeri, F.A., Kalantari, M.,
Zarifi, M., Ghalebi, S.R. and Mehrabani, D. (2007)
Seroprevalence of Toxoplasma gondii among caprines in Fars
province, Southern Iran. J. Vet. Parasitol., 21(2): 153-155. |
|
|
18. Azizi, H., Shiran, B., Boroujeni, A.B. and Jafari, M.
(2014) Molecular survey of Toxoplasma gondii in sheep,
cattle and meat products in Chaharmahal va Bakhtiari
Province, Southwest of Iran. Iran. J. Parasitol., 9(3):
429-434.
PMid:25678929 PMCid:PMC4316576 |
|
|
19. Santos, S.L., de Souza Costa, K., Gondim, L.Q., da
Silva, M.S.A., Uzêda, R.S., Abe-Sandes, K. and Gondim,
L.F.P. (2010) Investigation of Neospora caninum, Hammondia
sp. and Toxoplasma gondii in tissues from slaughtered beef
cattle in Bahia, Brazil. Parasitol. Res., 106(2): 457-461.
http://dx.doi.org/10.1007/s00436-009-1686-4
PMid:19943064 |
|
|
|
20. Olivier, A., Herbert, B., Sava, B., Pierre, C., John,
D.C. and Aline, D.K. (2007) Surveillance and monitoring of
toxoplasma in humans, food and animals: A scientific opinion
of the panel on biological hazards. EFSA. J., 583: 1-64. |
|
|
21. Tenter, A.M., Heckeroth, A.R., and Weiss, L.M. (2000)
Toxoplasma gondii: From animals to humans. Int. J. Parasitol.,
30(12): 1217-1258.
http://dx.doi.org/10.1016/S0020-7519(00)00124-7 |
|
|
22. Elmore, S.A., Jones, J.L., Conrad, P.A., Patton, S.,
Lindsay, D.S. and Dubey, J.P. (2010) Toxoplasma gondii:
Epidemiology, feline clinical aspects, and prevention.
Trends. Parasitol., 26(4): 190-196.
http://dx.doi.org/10.1016/j.pt.2010.01.009
PMid:20202907 |
|
|
23. Derakhshan, M. and Mousavi, M. (2014) Serological survey
of antibodies to Toxoplasma gondii in cats, goats, and sheep
in Kerman, Iran. Comp. Clin. Path., 23(2): 267-268.
http://dx.doi.org/10.1007/s00580-012-1605-4 |
|
|
24. Fayer, R. (1981) Toxoplasmosis update and public health
implications. Can. Vet. J., 22(11): 344-352.
PMid:7337909 PMCid:PMC1789993 |
|
|
25. Raeghi, S., Akaberi, A. and Sedeghi, S. (2011)
Seroprevalence of Toxoplasma gondii in sheep, cattle and
horses in Urmia North-West of Iran. Iran. J. Parasitol.,
6(4): 90-94.
PMid:22347318 PMCid:PMC3279903 |
|
|
26. Bonyadian, M., Hematzade, F. and Manuchehri, K. (2007)
Seroprevalence of antibodies to Toxoplasma gondii in sheep
in center of Iran. Pak. J. Biol. Sci., 10(18): 3228-3230.
http://dx.doi.org/10.3923/pjbs.2007.3228.3230
PMid:19090132 |
|
|
27. Khezri, M., Mohammadian, B., Esmailnia, K. and Khezri,
O. (2012) Toxoplasmosis in sheep from Kurdistan Province,
Iran. Afr. J. Microbiol. Res., 6(18): 3989-3992.
http://dx.doi.org/10.3923/ajas.2012.182.188 |
|
|
28. Hashemi-Fesharki, R. (1996) Seroprevalence of Toxoplasma
gondii in cattle, sheep and goats in Iran. Vet. Parasitol.,
61(1): 1-3.
http://dx.doi.org/10.1016/0304-4017(95)00818-7 |
|
|
29. Asgari, Q., Sarkari, B., Amerinia, M., Panahi, S.,
Mohammadpour, I. and Sarvestani, A.S. (2013) Toxoplasma
infection in farm animals: A seroepidemiological survey in
Fars province, South of Iran. Jundishapur. J. Microbiol.,
6(3): 269-272.
http://dx.doi.org/10.5812/jjm.5195 |
|
|
30. Van der Puije, W.N.A., Bosompem, K.M., Canacoo, E.A.,
Wastling, J.M. and Akanmori, B.D. (2000) The prevalence of
anti - Toxoplasma gondii antibodies in Ghanaian sheep and
goats. Acta Trop., 76(1): 21-26.
http://dx.doi.org/10.1016/S0001-706X(00)00084-X |
|
|
31. Tzanidakis, N., Maksimov, P., Conraths, F.J., Kiossis,
E., Brozos, C., Sotiraki, S. and Schares, G. (2012)
Toxoplasma gondii in sheep and goats: Seroprevalence and
potential risk factors under dairy husbandry practices. Vet.
Parasitol., 190(3): 340-348.
http://dx.doi.org/10.1016/j.vetpar.2012.07.020
PMid:22883972 |
|
|
32. Lopes, A.P., Dubey, J.P., Neto, F., Rodrigues, A.,
Martins, T., Rodrigues, M. and Cardoso, L. (2013)
Seroprevalence of Toxoplasma gondii infection in cattle,
sheep, goats and pigs from the North of Portugal for human
consumption. Vet. Parasitol., 193(1): 266-269.
http://dx.doi.org/10.1016/j.vetpar.2012.12.001
PMid:23290614 |
|
|
|
33. Kotula, A.W., Dubey, J.P., Sharar, A.K., Andrews, C.D.,
Shen, S.K. and Lindsay, D.S. (1991) Effect of freezing on
infectivity of Toxoplasma gondii tissue cysts in pork. J.
Food Prot., 54(9): 687-690. |
|
|
34. Dubey, J.P., Kotula, A.W., Sharar, A., Andrews, C.D. and
Lindsay, D.S. (1990) Effect of high temperature on
infectivity of Toxoplasma gondii tissue cysts in pork. The
J. Parasitol., 76(2): 201-204.
http://dx.doi.org/10.2307/3283016 |
|
|
35. Dumètre, A., Ajzenberg, D., Rozette, L., Mercier, A. and
Dardé, M.L. (2006) Toxoplasma gondii infection in sheep from
Haute-Vienne, France: Seroprevalence and isolate genotyping
by microsatellite analysis. Vet. Parasitol., 142(3):
376-379.
http://dx.doi.org/10.1016/j.vetpar.2006.07.005
PMid:16919879 |
|
|
36. Shaapan, R.M., El-Nawawi, F.A. and Tawfik, M.A.A. (2008)
Sensitivity and specificity of various serological tests for
the detection of Toxoplasma gondii infection in naturally
infected sheep. Vet. Parasitol., 153(3): 359-362.
http://dx.doi.org/10.1016/j.vetpar.2008.02.016
PMid:18406534 |
|
