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Research (Published online: 13-12-2016)

12. Immunotoxic effect of thiamethoxam in immunized mice with Brucella abortus cultural filtrate antigen - L. H. Salema, M. J. Alwan and Afaf Abdulrahman Yousif

Veterinary World, 9(12): 1407-1412

 

 

   doi: 10.14202/vetworld.2016.1407-1412

 

L. H. Salema: Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Baghdad, Iraq; Ministry of High Education and Scientific Research, Baghdad, Iraq; salema.l@covm.uobaghdad.edu.iq

M. J. Alwan: Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Baghdad, Iraq; Ministry of High Education and Scientific Research, Baghdad, Iraq; mohammed44@yahoo.com

Afaf Abdulrahman Yousif: Ministry of High Education and Scientific Research, Baghdad, Iraq; Department of Internal and Preventive Veterinary Medicine, University of Baghdad, Baghdad, Iraq; afaf_a.rahman@yahoo.com

 

Received: 06-06-2016, Accepted: 09-11-2016, Published online: 13-12-2016

 

Corresponding author: Afaf Abdulrahman Yousif, e-mail: afaf_a.rahman@yahoo.com


Citation: Salema LH, Alwan MJ, Yousif AA (2016) Immunotoxic effect of thiamethoxam in immunized mice with Brucella abortus cultural filtrate antigen, Veterinary World, 9(12): 1407-1412.



Aim: This study was planned for determination the toxic effect of thiamethoxam (TMX) in immunized mice with Brucella abortus culture filtrate antigen (CFBAgs) (as a vaccine) and its role of TMX on decrease activity of B. abortus antigen on eliciting of humoral and cellular immunity.

Materials and Methods: To achieve these goals 60 female mice were used, 7-8 weeks age, they were divided equally into three groups (20 in each group) and treated as follows: 1st group: Mice were immunized with CFBAgs intraperitoneally in two doses, 2 weeks intervals with (protein concentration 2 mg\ml), 2nd group: Mice immunized as in the 1st group and was administrated orally with 1/10 lethal dose 50% of TMX (83.7 mg/kg B.W.) for 4 weeks daily, 3rd group was administrated orally with 0.3 ml normal saline served as a control group. At day 28 post immunization (PI) delayed type hypersensitivity (skin test) was done, and serum samples were collected at day 30 (PI) for detection of passive hemagglutination test (PHA); interferon gamma (IFN-γ) which was done by enzyme-linked immunosorbent assay test in addition to phagocytes assay.

Results: The results of skin test post injection with soluble antigen of B. abortus intradermally showed a high significantly mean values at p≤0.05 of footpad skin thickness in the 1st group of mice which recorded (0.51▒0.002 mm) as compared with the 2nd group of mice which showed (0.08▒0.002 mm) after 24 h; the mean values of skin thickness were declined in the 1st mice (0.46▒0.002) and 2nd mice (0.070▒0.001) at 48 h; control group showed a negative results. These results were agreed with results of serum levels of IFN-γ (pg/ml) that showed that a significant increase the vaccinated 1st group (406.36▒1.52), than those values in the 2nd group (151.61▒0.89) and negative result in 3rd group (46.47▒0.60), in addition to results of PHA test which showed a significant increase in antibody titer in the 1st group (139▒12.16) with low level of serum antibody in the 2nd group (7.66▒0.33). Phagocytic ratio results in the 1st group showed an increase to reach (18.55▒0.44) than a ratio in the 2nd group (13.24▒0.32) and the control group (5.46▒0.25).

Conclusion: It was concluded that TMX induced suppression of humoral and cellular immune responses in immunized mice with CFBAgs.

Keywords: Brucella abortus, enzyme-linked immunosorbent assay test, interferon gamma, phagocyte assay, skin test, thiamethoxam.



1. Maienfisch, P., Angst, M., Brandl, F., Fischer, W., Hofer, D., Kayser, H., Kobel, W., Rindlisbacher, A., Senn, R., Steinemann, A. and Widmer, H. (2001) Chemistry and biology of thiamethoxam: A second generation neonicotinoid. Pest Manag. Sci., 57: 906-913.
https://doi.org/10.1002/ps.365
PMid:11695183
 
2. Ford, K.A. and Casida, J.E. (2008) Comparative metabolism and pharmacokinetics of seven neonicotinoid insecticides in spinach. J. Agric. Food Chem., 56(21): 10168-10175.
https://doi.org/10.1021/jf8020909
PMid:18922014
 
3. Kurwadkar, S., Evans, A., DeWinne, D., White, P. and Mitchell, F. (2016) Modeling photodegradation kinetics of three systemic neonicotinoids - Dinotefuran, imidacloprid andthiamethoxam in aqueous and soil environment. Environ. Toxicol. Chem., 35(7): 1718-1726.
https://doi.org/10.1002/etc.3335
PMid:26660507
 
4. Tomizawa, M. and Casida, J.E. (2005) Neonicotinoid insecticide toxicology: Mechanisms of selective action. Annu. Rev. Pharmacol. Toxicol., 45: 247-268.
https://doi.org/10.1146/annurev.pharmtox.45.120403.095930
PMid:15822177
 
5. Sinha, S. and Thaker, A.M. (2014) Study on the impact of lead acetate pollutant on immunotoxicity produced by thiamethoxam pesticide. Indian J. Pharmacol., 46(6): 596-600.
https://doi.org/10.4103/0253-7613.144910
PMid:25538329 PMCid:PMC4264073
 
6. Di Prisco, G., Cavaliere, V., Annoscia, D., Varricchio, P., Caprio, E., Nazzi, F., Gargiulo, G. and Pennacchio, F. (2013) Neonicotinoid clothianidin adversely affects insect immunity and promotes replication of a viral pathogen in honey bees. Proc. Natl. Acad. Sci. U S A, 110(46): 18466-18471.
https://doi.org/10.1073/pnas.1314923110
PMid:24145453 PMCid:PMC3831983
 
7. Elaine, M.S., Sriranganathan, D.N. and Lage, A.P. (2015) Recent advances in Brucella abortus vaccines. Vet. Res., 46(76): 1-10.
 
8. Truong, Q.L., Cho, Y., Park, S., Park, B.K. and Hahn, T.W. (2016) Brucella abortus mutants lacking ATP-binding cassette transporter proteins are highly attenuated in virulence and confer protective immunity against virulent B. Abortus challenge in BALB/c mice. Microb. Pathog., 95: 175-185.
https://doi.org/10.1016/j.micpath.2016.04.009
PMid:27057678
 
9. Hop, H.T., Reyes, A.W., Simborio, H.L., Arayan, L.T., Min, W.G., Lee, H.J., Lee, J.J., Chang, H.H. and Kim, S. (2016) Immunization of mice with recombinant Brucella abortus organic hydroperoxide resistance (Ohr) protein protects against a virulent Brucella abortus 544 Infection. J. Microbiol. Biotechnol., 26(1): 190-196.
https://doi.org/10.4014/jmb.1505.05028
PMid:26464379
 
10. Dorneles, E.M., Teixeira-Carvalho, A., Ara˙jo, M.S., Sriranganathan, N. and Lage, A.P. (2015) Immune response triggered by Brucella abortus following infection or vaccination. Vaccine, 33(31): 3659-3666.
https://doi.org/10.1016/j.vaccine.2015.05.057
PMid:26048781
 
11. Engel, P., Martinson, V.G. and Moran, N.A. (2012) Functional diversity within the simple gut microbiota of the honey bee. Proc. Natl. Acad. Sci. U S A, 109(27): 11002-11007.
https://doi.org/10.1073/pnas.1202970109
PMid:22711827 PMCid:PMC3390884
 
12. Dirwal, A.R., Alwan, M.J. and Falih, A.B. (2014) Toxopathological and immunotoxical effects of thiamethoxam in white mice. AL-Qadisiya J. Vet. Med. Sci., 13(1): 15-24.
 
13. Gawade, L., Dadarkar, S.S., Husain, R. and Gatne, M. (2013) A detailed study of developmental immunotoxicity of imidacloprid in Wistar rats. Food Chem. Toxicol., 51: 61-70.
https://doi.org/10.1016/j.fct.2012.09.009
PMid:23000444
 
14. Badgujar, P.C., Jain, S.K., Singh, A., Punia, J.S., Gupta, R.P. and Chandratre, G.A. (2013) Immunotoxic effects of imidacloprid following 28 days of oral exposure in BALB/c mice. Environ. Toxicol. Pharmacol., 35(3): 408-418.
https://doi.org/10.1016/j.etap.2013.01.012
PMid:23467117
 
15. Dixon, W.J. (1980) Efficient analysis of experimental observations. Annu. Res. Pharmacol. Toxicol., 20: 441-462.
https://doi.org/10.1146/annurev.pa.20.040180.002301
PMid:7387124
 
16. Mitov, I., Denchen, V. and Linde, K. (1992) Humoral and cell mediated immunity in mice after immunization with live oral vaccines of Salmonella typhimurium anxotrophic mutants with two attenuating markers. Vaccine, 10: 61-66.
https://doi.org/10.1016/0264-410X(92)90421-F
 
17. Hudson, L. and Hay, F.C. (1980) Practical Immunology. 3rd ed. Black Well Scientific Publication, Oxford, London. p98-105.
 
18. Herbert, W.J. (1978) Passive haemagglutination with special reference to the tanned cell technique. Cellular immunology. In: Weir, D.M., editor. Handbook of Experimental Immunology. 3rd ed., Vol. II, Ch. 20. Blackwell Scientific Publication, Oxford. p1-20.
 
19. Cheng, S. and Lamont, S.J. (1988) Genetic analysis of immunocompetence measures in a white Leghorn chicken line. Poult. Sci., 67: 989-995.
https://doi.org/10.3382/ps.0670989
PMid:3265514
 
20. Chao, C.H. and Lee, U.P. (2001) Relationship between reproductive performance and immunity in Taiwan country chickens. Poult. Sci., 80: 535-540.
https://doi.org/10.1093/ps/80.5.535
PMid:11372700
 
21. Allen, J.W., Shuler, C.F., Mendes, R.W. and Latt, S.A. (1977) A simplified technique for in vivo analysis of sister chromatid exchanges using 5-bromo deoxyuridine tablets. Cytogenet. Cell Genet., 18: 231-237.
https://doi.org/10.1159/000130765
PMid:872628
 
22. Oliveira, S.C. and Splitter, A.G. (1995) CD8+ Type 1 CD44hi CD45 RBlo T lymphocytes control intracellular Brucella abortus infection as demonstrated in major histocompatibility complex class I- And class II-deficient mice. Eur. J. Immunol., 25: 2551-2557.
https://doi.org/10.1002/eji.1830250922
PMid:7589125
 
23. Ramzi, S.C., Vinay, K. and Stanley, R. (1994) Pathologic Basis of Diseases. Vol. 5. WB Saunders Company, Philadelphia, PA. p86.
 
24. Khan, A.L.A. and Khan, M.Z. (2012) Hemato-biochemical changes induced by pyrethroid insecticides in avian, fish and mammalian species. Int. J. Agric. Biol., 14: 834-842.
 
25. Mohamed, M., Gamal, B., Inas, R. and Mostafa, E. (2011) Immunological and histological effects of exposure to imidacloprid insecticide in male albino rats. Afr. J. Pharm. Pharmacol., 5(18): 2106-2114.
https://doi.org/10.5897/AJPP11.625
 
26. Maecker, H.T., Umetsu, D.T., DeKruyff, R.H. and Levy, S.H. (1998) Cytotoxic T cell responses to DNA vaccination: Dependence on antigen presentation via class II MHC. J. Immunol., 161: 6532-6536.
PMid:9862678
 
27. Zhan, Y. and Cheers, C. (1995) Endogenous interleukin-12 is involved in resistance to Brucella abortus infection. Infect. Immun., 63(4): 1387-1390.
PMid:7890399 PMCid:PMC173163
 
28. Mahajan, N.K., Kulshreshtha, R.C., Malik, G. and Dahiya, J.P. (2005) Immunogenicity of major cell surface protein(s) of Brucella melitensis Rev 1. J. Res. Commun., 29(3): 189-199.
https://doi.org/10.1023/b:verc.0000047500.20855.7a
 
29. Hsieh, C.S., Macatonia, S.E., Tripp, C.S., Wolf, S.F., O'Garra, A. and Murphy, K.M. (1993) Development of TH1 CD4+T cells through IL-12 produced by Listeria-induced macrophages. Science, 260: 547-549.
https://doi.org/10.1126/science.8097338
PMid:8097338
 
30. Jeong, H.Y., Mitchell, V.A. and Vaughan, C.W. (2012) Role of 5-HT1 receptor subtypes in the modulation of pain and synaptic transmission in rat superficial dorsal horn. Br. J. Pharmacol., 165: 1956-1965.
https://doi.org/10.1111/j.1476-5381.2011.01685.x
PMid:21950560 PMCid:PMC3372843