Open Access
Research
(Published
online: 14-12-2016)
13.
Evidence of hemolysis in pigs infected with
highly virulent African swine fever virus -
Zaven Karalyan, Hovakim Zakaryan, Elina Arakelova, Violeta
Aivazyan, Marina Tatoyan, Armen Kotsinyan, Roza Izmailyan and
Elena Karalova
Veterinary World, 9(12): 1413-1419
doi:
10.14202/vetworld.2016.1413-1419
Zaven Karalyan:
Laboratory of Cell Biology and Virology, Institute of Molecular
Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia;
zkaralyan@yahoo.com
Hovakim Zakaryan:
Laboratory of Cell Biology and Virology, Institute of Molecular
Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia;
hovakimzakaryan@gmail.com
Elina Arakelova:
Laboratory of Human Genomics and Immunomics, Institute of
Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan,
Armenia; elinaa72@mail.ru
Violeta Aivazyan:
Laboratory of Human Genomics and Immunomics, Institute of
Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan,
Armenia;
viola_ay@yahoo.com
Marina Tatoyan:
Laboratory of Cell Biology and Virology, Institute of Molecular
Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia;
marina.tatoyan@mail.ru
Armen Kotsinyan:
Laboratory of Cell Biology and Virology, Institute of Molecular
Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia;
armenrkotsinyan@gmail.com
Roza Izmailyan:
Laboratory of Cell Biology and Virology, Institute of Molecular
Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia;
izmruzanna@gmail.com
Elena Karalova:
Laboratory of Cell Biology and Virology, Institute of Molecular
Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia;
z_karalyan@mb.sci.am
Received: 29-08-2016, Accepted: 12-11-2016, Published online:
14-12-2016
Corresponding author:
Zaven Karalyan, e-mail: zkaralyan@yahoo.com
Citation:
Karalyan Z, Zakaryan H, Arakelova E, Aivazyan V, Tatoyan M,
Kotsinyan A, Izmailyan R, Karalova E (2016) Evidence of hemolysis
in pigs infected with highly virulent African swine fever virus,
Veterinary World, 9(12):
1413-1419.
Abstract
Aim:
The research was conducted to understand more profoundly the
pathogenetic aspects of the acute form of the African swine fever
(ASF).
Materials and Methods:
A
total of 10 pigs were inoculated with ASF virus (ASFV) (genotype
II) in the study of the red blood cells (RBCs), blood and urine
biochemistry in the dynamics of disease.
Results:
The major hematological differences observed in ASFV infected pigs
were that the mean corpuscular volume, mean corpuscular
hemoglobin, and hematocrits were significantly decreased compared
to controls, and the levels of erythropoietin were significantly
increased. Also were detected the trends of decrease in RBC count
at terminal stages of ASF. Analysis of blood biochemistry revealed
that during ASF development, besides bilirubinemia significantly
elevated levels of lactate dehydrogenase, and aspartate
aminotransferase were detected. Analysis of urine biochemistry
revealed the presence of bilirubinuria, proteinuria during ASF
development. Proteinuria, especially at late stages of the disease
reflects a severe kidney damage possible glomerulonefritis.
Conclusion:
The results of this study indicate the characteristics of
developing hemolytic anemia observed in acute ASF (genotype II).
Keywords:
African swine fever virus, bilirubinemia, bilirubinuria,
erythropoietin, hemolysis, proteinuria.
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