Open Access
Research
(Published
online: 18-12-2016)
17.
Evaluation of sperm recovered after
slaughter from cauda epididymides of red Sokoto bucks -
A. H. Abu, A. I. Kisani and T. Ahemen
Veterinary World, 9(12): 1440-1444
doi:
10.14202/vetworld.2016.1440-1444
A. H. Abu:
Department of Veterinary Physiology, Pharmacology and
Biochemistry, College of Veterinary Medicine, Federal University
of Agriculture, P.M.B. 2373, Makurdi, Benue State, Nigeria;
adakoleabu1@gmail.com
A. I. Kisani:
Department of Veterinary Surgery and Theriogenology, College of
Veterinary Medicine, Federal University of Agriculture, P.M.B.
2373, Makurdi, Benue State, Nigeria; abohkisani@yahoo.com
T. Ahemen:
Department of Animal Breeding and Physiology, College of Animal
Science, Federal University of Agriculture, P.M.B. 2373, Makurdi,
Benue State, Nigeria; drtahemen@gmail.com
Received: 11-08-2016, Accepted: 15-11-2016, Published online:
18-12-2016
Corresponding author:
A. H. Abu, e-mail: adakoleabu1@gmail.com
Citation:
Abu AH, Kisani AI, Ahemen T (2016) Evaluation of sperm recovered
after slaughter from cauda epididymides of red Sokoto bucks,
Veterinary World, 9(12):
1440-1444.
Abstract
Aim:
Viable spermatozoa could be recovered from the cauda epididymides
for the purpose of preservation of genetic material of male
animals with desirable traits and for use in reproductive
biotechnology. The aim of this study was to determine the effect
of storage time on testicular and epididymal biometry, sperm
reserves and epididymal sperm characteristics of red Sokoto bucks
post mortem.
Materials and Methods:
Testes-epididymides were collected immediately after slaughter of
mature red Sokoto bucks and trans-ported in ice chest to the
laboratory. The samples were either processed immediately or
stored at 5°C in refrigerator for 24, 48 h and then processed. The
testes and epididymides were measured and weighed. Sperm motility,
concentration, livability, morphology, intact acrosome from different treatment groups including control were
evaluated and means (±standard error of mean) were recorded.
Results:
There was no significant difference (p>0.05) in the testicular and
epididymal dimensions determined between the means of the groups.
Percent sperm motility and viability decreased significantly
(p<0.05) after 24 h from 69.00±0.46 and 71.27±0.50% to 50.60±0.48
and 60.47±0.70% at 48 h, respectively. Significant decreases
(p<0.05) in epididymal sperm concentration and intact acrosome
from 2.86±0.08 and 92.87±0.39 at 0 to 24 h of storage,
respectively, were observed.
Conclusion:
The results of this study suggest that spermatozoa recovered from
the epididymides of red Sokoto bucks were viable after storage for
up to 48 h. Furthermore, this finding offers some hope that
epididymal sperm recovered post-mortem can be used in assisted
reproductive technologies.
Keywords:
epididymides, goat bucks, spermatozoa, testes.
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