Open Access
Research
(Published
online: 18-12-2016)
18.
Detection, identification, and
differentiation of sheep pox virus and goat pox virus from
clinical cases in Giza Governorate, Egypt -
M. A. Mahmoud and M. H. Khafagi
Veterinary World, 9(12): 1445-1449
doi:
10.14202/vetworld.2016.1445-1449
M. A. Mahmoud:
Department of Parasitology and Animal Diseases, Veterinary
Research Division, National Research Centre, Dokki 12622, Giza,
Egypt; m_elfatatri@yahoo.com
M. H. Khafagi:
Department of Parasitology and Animal Diseases, Veterinary
Research Division, National Research Centre, Dokki 12622, Giza,
Egypt; manalhamdy41@yahoo.com
Received: 20-08-2016, Accepted: 16-11-2016, Published online:
18-12-2016
Corresponding author:
M. A. Mahmoud, e-mail: m_elfatatri@yahoo.com
Citation:
Mahmoud MA, Khafagi MH (2016) Detection, identification, and
differentiation of sheep pox virus and goat pox virus from
clinical cases in Giza Governorate, Egypt,
Veterinary World, 9(12):1445-1449.
Abstract
Aim:
To isolate, identify, and differentiate
Capripoxviruses
(CaPV)
(sheep pox virus and goat pox virus) infections by egg
inoculation, transmission electron microscopy (TEM), and 30 kDa
RNA polymerase subunit gene-based polymerase chain reaction (PCR)
(RPO30) in clinically affected animals in Hawamdia township of
Giza Governorate, Egypt.
Materials and Methods:
A
total of 37 scab samples were collected from clinically suspected
field cases of sheep pox and goat pox. These samples were
collected during (2014-2015) during different outbreaks of sheep
pox and goat pox from Hawamdia township of Giza Governorate,
Egypt. The samples were subjected to egg inoculation, TEM, and
(RPO30) gene-based PCR. By using the egg inoculation: Previously
prepared 37 scab samples (n=23 sheep and n=14 goats) were
inoculated on the chorioallantoic membrane of specific pathogen
free (SPF) embryonated chicken eggs (12 days old age). In the
presence of the suitable percentage of humidity and candling, the
inoculated eggs were incubated at 37°C. By using the TEM: Samples
showed positive pock lesions on the chorioallantoic membranes,
were fixed in glutaraldehyde, then processed and sectioned for
TEM. Using the (RPO30) gene-based PCR assay, 30 of positive
samples after egg inoculation (n=19 sheep and n=11 goats) were
screened.
Results:
Using the egg inoculation, a characteristic pock lesions for
poxviruses were seen in 30/37 (n=19 sheep and n=11 goats)
(81.08%). Using the TEM, examination of the positive samples after
egg inoculation revealed positive result in 23/30 (n=15 sheep and
n=8 goats) (76.66%). The positive results represented by the
presence of negatively stained oval-shape virus particles. Using
the (RPO30) gene-based PCR assay, out of 30 total of positive
samples after egg inoculation (n=19 sheep and n=11 goats) were
screened, 27 (90%) samples (n=17 sheep and n=10 goats) were
positive. The given band sizes of sheep and goats were 172 and 152
bp, respectively.
Conclusion:
PCR assay depended on RPO30 gene can be used lonely for the
detection, identification, and differentiation of CaPVs. RPO30
gene-based PCR assay in combination with gene sequencing helps in
molecular epidemiological studies of CaPV infection.
Keywords:
Capripoxvirus, polymerase chain reaction, goat pox, isolation,
RPO30 gene, sheep pox, transmission electron microscope.
References
1. Tulman, E.R., Afonso, C.L., Lu, Z., Zsak, L., Sur, J.H.,
Sandybaev, N.T., Kerembekova, U.Z., Zaitsev, V.L., Kutish, G.F.
and Rock, D.L. (2002) The genomes of sheeppox and goatpox
viruses. J. Virol., 76: 6054-6061.
https://doi.org/10.1128/JVI.76.12.6054-6061.2002
PMid:12021338 PMCid:PMC136203 |
|
|
2. Kitching, R.P. (1999) Capripoxviruses. In: Granoff, A. and
Webster, R.G., editors. Encyclopedia of Virology. Vol. 3.
Academic Press, San Diego, CA. p1376-1381.
https://doi.org/10.1006/rwvi.1999.0231 |
|
|
3. Hani, B., Thang, T., Shawn, B. and Maged, G. (2015)
Seroprevalence of sheep and goat pox, peste des petits
ruminants and rift valley fever in Saudi Arabia. PLoS One,
10(10): e0140328.
https://doi.org/10.1371/journal.pone.0140328
PMid:26462199 PMCid:PMC4604144 |
|
|
4. Kitching, R.P. and Taylor, W.P. (1985) Transmission of
Capripoxvirus. Res. Vet. Sci., 39(2): 196-199.
PMid:2999929 |
|
|
5. Zelalem, A., Degefu, H., Getachew, G. and Menbere, K.
(2015) Seroprevalence of lumpy skin disease in selected
districts of West Wollega zone, Ethiopia. BMC Vet. Res., 11:
135.
https://doi.org/10.1186/s12917-015-0432-7
PMid:26082259 PMCid:PMC4468805 |
|
|
6. Verma, S., Verma, L.K., Gupta, V.K., Katoch, V.C., Dogra,
V., Pal, B. and Sharma, M. (2011) Emerging Capripoxvirus
disease outbreaks in Himachal Pradesh, a Northern state of
India. Transbound. Emerg. Dis., 58: 79-85.
https://doi.org/10.1111/j.1865-1682.2010.01172.x
PMid:21214867 |
|
|
7. Venkatesan, G., Bhanuprakash, V., Balamurugan, V., Bora,
D.P., Prabhu, M., Yogisharadhya, R. and Pandey, A.B. (2012)
Rapid detection and quantification of Orf virus from infected
scab materials of sheep and goats. Acta Virol., 56: 81-83.
https://doi.org/10.4149/av_2012_01_81
PMid:22404614 |
|
|
8. Maksyutov, R.A., Gavrilova, E.V., Agafonov, A.P., Taranov,
O.S., Glotov, A.G., Miheev, V.N., Shchelkunov, S.N. and
Sergeev, A.N. (2013) An outbreak of sheep pox in Zabajkalskij
kray of Russia. Transbound. Emerg. Dis., 62(4): 453-456.
https://doi.org/10.1111/tbed.12176
PMid:24127821 |
|
|
9. Davies, F.G. (1976) Characteristics of a virus causing a
pox disease in sheep and goats in Kenya, with observation on
the epidemiology and control. J. Hyg. (London), 76: 163-171.
https://doi.org/10.1017/S0022172400055066 |
|
|
10. Sherry, L.H., Chen, P., McFadden, G. and Rothenburg, R.S.
(2014) Poxviruses and the evolution of host range and
virulence. Infect. Genet. Evol., 21: 15-40.
https://doi.org/10.1016/j.meegid.2013.10.014
PMid:24161410 PMCid:PMC3945082 |
|
|
11. Diallo, A. and Viljoen, G.J. (2007) Genus Capripoxvirus.
In: Mercer, A.A., Schmidt, A. and Weber, O., editors.
Poxviruses. Birkhauser, Basel, Switzerland. p167-181.
https://doi.org/10.1007/978-3-7643-7557-7_8 |
|
|
12. Balinsky, C.A., Delhon, G., Smoliga, G., Prarat, M.,
French, R.A., Geary, S.J., Rock, D.L. and Rodriguez, L.L.
(2008) Rapid preclinical detection of sheeppox virus by a
real-time PCR assay. J. Clin. Microbiol., 46: 438-442.
https://doi.org/10.1128/JCM.01953-07
PMid:18032617 PMCid:PMC2238129 |
|
|
13. Hailat, N., Al-Rawashdeh, O., Lafi, S. and Al-Bateineh, Z.
(1994) An outbreak of sheep pox associated with unusual winter
conditions in Jordan. Trop. Anim. Health Prod., 26: 79-80.
https://doi.org/10.1007/BF02239903
PMid:7941033 |
|
|
|
14. Office, OIE. (2014) Internationale des Epizooties (World
Health Organization for Animals) Manual of Diagnostic Tests
and Vaccines for Terrestrial Animals. Sheep Pox and Goat Pox
(Version Adopted in May 2010). Vol. 7.14. Ch. 2. OIE, Paris,
France 2014. |
|
|
15. Hosamani, M., Mondal, B., Tembhurne, P.A., Bandyo-Padhyay,
S.K., Singh, R.K. and Rasool, T.J. (2004) Differentiation of
sheep pox and goat poxviruses by sequence analysis and
PCR-RFLP of P32 gene. Virus Genes, 29: 73-80.
https://doi.org/10.1023/B:VIRU.0000032790.16751.13
PMid:15215685 |
|
|
16. Lamien, C.E., Lelenta, M., Goger, W., Silber, R.,
Tuppurainen, E., Matijevic, M., Luckins, A.G. and Diallo, A.
(2011) Real time PCR method for simultaneous detection,
quantitation and differentiation of Capripoxviruses. J. Virol.
Methods, 171: 134-140.
https://doi.org/10.1016/j.jviromet.2010.10.014
PMid:21029751 |
|
|
17. Zhixun, Z., Bin, F., Guohua, W., Xinmin, Y., Yingguo, L.,
Xiaoli, Z., Hua, Y., Xueling, D., Haixia, Z., Bo, T., Jian, L.
and Qiang, Z. (2014) Development of loop-mediated isothermal
amplification assay for specific and rapid detection of
differential goat pox virus and sheeppox virus. BMC Microbiol.,
14: 10.
https://doi.org/10.1186/1471-2180-14-10
PMid:24438089 PMCid:PMC3942189 |
|
|
18. Venkatesan, G., Balamuruganl, V., Yogisharadhya, R.,
Kumar, A. and Bhanuprakash, V. (2012) Differentiation of
sheeppox and goatpox viruses by polymerase chain
reaction-restriction fragment length polymorphism. Virol.
Sin., 6: 353-359.
https://doi.org/10.1007/s12250-012-3277-2 |
|
|
19. Tuppurainen, E.S., Venter, E.H. and Coetzer, J.A. (2005)
The detection of lumpy skin disease virus in samples of
experimentally infected cattle using different diagnostic
techniques. Onderstepoort J. Vet. Res., 72: 153-164.
https://doi.org/10.4102/ojvr.v72i2.213
PMid:16137133 |
|
|
20. Venkatesan, G., Balamurugan, V. and Bhanuprakash, V.
(2014) Multiplex PCR for simultaneous detection and
differentiation of sheeppox, goatpox and orf viruses from
clinical samples of sheep and goats. J. Virol. Methods, 195:
1-8.
https://doi.org/10.1016/j.jviromet.2013.10.009
PMid:24134940 |
|
|
21. Zhou, T., Jia, H., Chen, G., He, X., Fang, Y., Wang, X.,
Guan, Q., Zeng, S., Cui, Q. and Jing, Z. (2012) Phylogenetic
analysis of Chinese sheep pox and goat pox virus isolates.
Virol. J., 9: 25.
https://doi.org/10.1186/1743-422X-9-25
PMid:22264255 PMCid:PMC3398307 |
|
|
22. Tuppurainen, E., Eeva, S., Pearson, C., Bachanek-Bankowska,
K., Knowles, N. and Amareen, S. (2014) Characterization of
sheep pox virus vaccine for cattle against lumpy skin disease
virus. Antiviral Res., 109: 1-6.
https://doi.org/10.1016/j.antiviral.2014.06.009
PMid:24973760 PMCid:PMC4149609 |
|
|
23. Venkatesan, G., Balamurugan, V. and Bhanuprakash, V.
(2014) TaqMan based real-time duplex PCR for simultaneous
detection and quantitation of Capripox and orf virus genomes
in clinical samples. J. Virol. Methods, 201: 44-50.
https://doi.org/10.1016/j.jviromet.2014.02.007
PMid:24552953 |
|
|
24. Esayas, G., Euloge, L.C., Roland, S., Eeva, S.T., Reingard,
G.M. and Diallo, A. (2013) Development of a cost-effective
method for Capripoxvirus genotyping using snapback primer and
dsDNA intercalating dye. PLoS One, 8(10): e75971.
https://doi.org/10.1371/journal.pone.0075971
PMid:24116084 PMCid:PMC3792100 |
|
|
25. Mahmoud, A.M., Abdelrahman, A.K. and Soliman, H. (2010)
Molecular and virological studies on contagious pustular
dermatitis isolates from Egyptian sheep and goats. Res. Vet.
Sci., 89: 290-294.
https://doi.org/10.1016/j.rvsc.2010.02.019
PMid:20304450 |
|
|
|
26. Cohen, J., Bererhi, A., Ribero, M., Vincet, J. and
Delogneau, J. (1971) Electron microscopic study of the
morphogenesis of sheep pox virus in tissue culture. Ann. Inst.
Pasteur (Paris), 121(4): 569-577. |
|
|
27. Lamien, C.E., Le Goff, C., Silber, R., Wallace, B.D.,
Gulyaz, V., Tuppurainen, E., Madani, H., Caufour, P., Adami,
T., El Harrak, M., Luckins, A.G., Albina, E. and Diallo, A.
(2011) Use of the Capripoxvirus homologue of vaccinia virus 30
kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic
and genotyping target: development of a classical PCR method
to differentiate goat poxvirus from sheep poxvirus. Vet.
Microbiol., 149: 30-39.
https://doi.org/10.1016/j.vetmic.2010.09.038
PMid:21115310 |
|
|
28. SanthamaniR, YogisharadhyaR, Venkatesan G, Shivachandra
SB, Pandey AB and Ramakrishnan MA (2013) Detection and
differentiation of sheeppox virus and goatpox virus from
clinical samples using 30 kDa RNA polymerase subunit (RPO30)
gene based PCR, Vet. World 6(11): 923-925.
https://doi.org/10.14202/vetworld.2013.923-925 |
|
|
29. Yan, X.M., Chu, Y.F., Wu, G.H., Zhao, Z.X., Li, J., Zhu,
H.X. and Zhang, Q. (2012) An outbreak of sheep pox associated
with goat poxvirus in Gansu province of China. Vet. Microbiol.,
156: 425-428.
https://doi.org/10.1016/j.vetmic.2011.11.015
PMid:22169434 |
|
