Open Access
Research
(Published
online: 20-12-2016)
20.
Multiple infections of
Anaplasma platys
variants in Philippine dogs -
Adrian Patalinghug Ybañez, Rochelle Haidee Daclan Ybañez, Naoaki
Yokoyama and Hisashi Inokuma
Veterinary World, 9(12): 1456-1460
doi:
10.14202/vetworld.2016.1456-1460
Adrian Patalinghug Ybañez:
Department of Biology and Environmental Studies, College of
Sciences, University of the Philippines Cebu, Lahug, Cebu City
6000, Philippines; Department of Research, Gullas College of
Medicine, University of the Visayas, Banilad, Mandaue City 6014,
Cebu, Philippines; Department of Veterinary Clinical Science,
Obihiro University of Agriculture and Veterinary Medicine,
Obihiro, Inada Cho, Hokkaido 080-8555 Japan; dr.adrianpybanez@gmail.com
Rochelle Haidee Daclan Ybañez:
Department of Biology and Environmental Studies, College of
Sciences, University of the Philippines Cebu, Lahug, Cebu City
6000, Philippines; rochelledybanez@gmail.com
Naoaki Yokoyama:
Research Unit for Molecular Diagnosis, National Research Center
for Protozoan Diseases, Obihiro University of Agriculture and
Veterinary Medicine, Obihiro, Inada Cho, Hokkaido 080-8555, Japan;
yokoyama@obihiro.ac.jp
Hisashi Inokuma:
Department of Veterinary Clinical Science, Obihiro University of
Agriculture and Veterinary Medicine, Obihiro, Inada Cho, Hokkaido
080-8555 Japan; inokuma@obihiro.ac.jp
Received: 25-07-2016, Accepted: 23-11-2016, Published online:
20-12-2016
Corresponding author:
Adrian Patalinghug Ybañez, e-mail: dr.adrianpybanez@gmail.com
Citation:
Ybañez AP, Ybañez RHD, Yokoyama N, Inokuma H (2016) Multiple
infections of
Anaplasma platys
variants in Philippine dogs,
Veterinary World, 9(12):
1456-1460.
Abstract
Aim:
Anaplasma platys,
the causative agent of infectious canine cyclic thrombocytopenia,
is a tick-borne pathogen that also has been implicated as
potentially zoonotic. To provide molecular evidence on the
multiple infections of
A. platys
variants in Philippine dogs.
Materials and Methods:
DNA fragments of
A. platys
from infected dogs in the Philippines were molecularly
characterized. For screening, 25 dogs suspected to have canine
anaplasmosis were tested using a 16S rRNA-based nested polymerase
chain reaction (PCR). Infection was confirmed by sequencing of
positive amplicons. Second round PCR targeting a longer 16S rRNA
fragment was subsequently performed on the first round PCR
amplicons of the positive samples. Further characterization using
the heat-shock operon (groEL)
gene was also performed on the
A. platys-positive
samples.
Results:
10 16S rRNA sequences were obtained and found 99.6-100% identical
to each other and 99.6-99.7% identical to the closest registered
A. platys
sequences. On the other hand, 36
groEL
clone sequences were obtained and found to be 85.1-99.8% identical
with each other and 85.0-88.9% identical to the closest previously
registered
A. platys
sequences. Four dogs were found coinfected with 2-3
groEL
variant sequences. Phylogenetic analyses suggest that the detected
A. platys
in the Philippines may represent unique variants.
Conclusion:
A. platys
variants were detected in Philippine dogs. Coinfection of
different
A. platys
variants in dogs was also demonstrated. The present study may
indicate the potential genetic diversity of
A. platys
in the country.
Keywords:
Anaplasma platys,
dogs, Philippines, variants.
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