Open Access
Research
(Published
online: 05-12-2016)
5.
Phylogenetic analysis of glycoprotein B gene
sequences of bovine herpesvirus 1 isolates from India reveals the
predominance of subtype 1.1. -
S. S. Patil, A. Prajapati, D. Hemadri, K. P. Suresh, G. S. Desai,
G. B. Manjunatha Reddy, B. M. Chandranaik, S. Ranganatha
and H. Rahman
Veterinary World, 9(12): 1364-1369
doi:
10.14202/vetworld.2016.1364-1369
S. S. Patil :
ICAR-National
Institute of Veterinary Epidemiology and Disease Informatics,
Bengaluru - 560 064, Karnataka, India; sharanspin123@rediffmail.com
A. Prajapati :
ICAR-National
Institute of Veterinary Epidemiology and Disease Informatics,
Bengaluru - 560 064, Karnataka, India; avi75prajapati@gmail.com
D. Hemadri :
ICAR-National
Institute of Veterinary Epidemiology and Disease Informatics,
Bengaluru - 560 064, Karnataka, India; divakar.hemadri@gmail.com
K. P. Suresh :
ICAR-National
Institute of Veterinary Epidemiology and Disease Informatics,
Bengaluru - 560 064, Karnataka, India; suresh.KP@icar.gov.in
G. S. Desai :
ICAR-National
Institute of Veterinary Epidemiology and Disease Informatics,
Bengaluru - 560 064, Karnataka, India; gurusdesai@rediffmail.com
G. B. Manjunatha Reddy :
ICAR-National
Institute of Veterinary Epidemiology and Disease Informatics,
Bengaluru - 560 064, Karnataka, India; suresh.KP@icar.gov.in
B. M. Chandranaik :
Institute of Animal
Health and Veterinary Biologicals, Bengaluru - 560 024, Karnataka,
India; dinglychandru@yahoo.co.in
S. Ranganatha :
Institute of Animal
Health and Veterinary Biologicals, Bengaluru - 560 024, Karnataka,
India; srangavetco@gmail.com
H. Rahman :
Division of Animal
Sciences, Indian Council of Agriculture Research, Krishi Bhawan,
New Delhi - 110 001, India; hricar@gmail.com
Received: 06-06-2016, Accepted: 17-10-2016, Published online:
05-12-2016
Corresponding author:
S. S. Patil,
e-mail: sharanspin123@rediffmail.com
Citation:
Patil SS, Prajapati A, Hemadri D, Suresh KP, Desai GS, Reddy GBM,
Chandranaik BM, Ranganatha S, Rahman H (2016) Phylogenetic
analysis of glycoprotein B gene sequences of bovine herpesvirus 1
isolates from India reveals the predominance of subtype 1.1.,
Veterinary World, 9(12): 1364-1369.
Abstract
Aim:
This study was
conducted for the isolation and molecular characterization of
bovine herpesvirus 1 (BoHV-1) isolated from the nasal and vaginal
swabs collected from naturally infected cattle showing clinical
symptoms of the respiratory disease.
Materials and
Methods:
Isolation of
BoHV-1 virus performed on clinical samples collected from 65
cattle from five states of India. The BoHV-1 isolates were further
confirmed by polymerase chain reaction (PCR) using primers
specific for glycoprotein B (gB) genomic region. PCR amplification
was performed using previously published gB gene-specific primer
pairs. gB PCR amplicons obtained from all isolates were sequenced,
and phylogenetic analysis was performed using software.
Results:
A total of 12
samples were found positive in cell culture isolation. 11 isolates
showed the visible cytopathic effect on Madin-Darby bovine kidney
after 72 h. Partial sequence analysis of gB gene of all isolates
revealed 99.0-100% homology between them. All isolates showed
99.2-99.8% homology with Cooper stain.
Conclusion:
BoHV-1.1 is the predominant circulating subtype of BoHV in India,
and all isolates have homology with Cooper stain.
Keywords:
bovine herpesvirus 1-1, glycoprotein B, India, infectious bovine
rhinotracheitis, phylogenetic analysis, sequencing, subtype 1.1.
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