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Research (Published online: 20-02-2016)

16. Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis - Mukul Anand and Sarvajeet Yadav

Veterinary World, 9(2): 203-206

 

 

   doi: 10.14202/vetworld.2016.203-206

 

 

Mukul Anand: Department of Veterinary Physiology, College of Veterinary Sciences and Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India; drmukulanandvet@gmail.com

Sarvajeet Yadav: Department of Veterinary Physiology, College of Veterinary Sciences and Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India; yadavsarvajeet24@gmail.com

 

Received: 21-08-2015, Revised: 03-01-2016, Accepted: 13-01-2016, Published online: 20-02-2016

 

Corresponding author: Mukul Anand, e-mail: drmukulanandvet@gmail.com


Citation: Anand M, Yadav S (2016) Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis, Veterinary World, 9(2): 203-206.



Aim: The aim was to determine the motion and kinematics characteristic of frozen-thawed spermatozoa in Sirohi goat using computer-assisted semen analysis.

Materials and Methods: A study was carried out in Sirohi buck. Semen collection was made biweekly from each buck with the help of artificial vagina. A total of 12 ejaculates were collected from two bucks (six ejaculates from each buck). Freshly collected semen was pooled and later evaluated. The pooled semen sample was extended with standard glycerolated egg yolk tris extender and later subjected to a process of cryopreservation. The motion and kinematic characteristics of spermatozoa were studied during freez-thawing process.

Results: Significantly (p<0.01) higher value of live percent, hypo-osmotic swelling test, and acrosomal integrity were recorded in neat semen followed by diluted and frozen thaw semen. The proportion of spermatozoa showing slow progression were the highest in the neat and diluted semen followed by rapid and non-progressively motile, while a reverse pattern was observed in the frozen thaw semen where the proportion of non-progressively motile spermatozoa were significantly (p<0.01) higher followed by slow and rapid progression.

Conclusion: This study showed that the best results for motion, vitality, plasma membrane integrity, and acrosome status were obtained in the neat semen followed by diluted and frozen thaw semen. Further, the process of cryopreservation results in a shift of motility from slow to non-progressive in the post-thaw semen with a significant decrease in the path velocities when compared to neat and diluted semen. Hence, it can be concluded that freezing-thawing process reduces the motility and kinematic characters spermatozoa and may be an important factor affecting the fertilizing ability of spermatozoa resulting in poor conception rate after insemination in goats.

Keywords: computer-assisted semen analysis, cryopreservation, motility, semen, Sirohi goat.



1. Jayashree, R., Jayashankar, M.R., Nagaraja, C.S., Satyanarayana, K. and Isloor, S. (2014) Goat rearing practices in southern Karnataka. Int. J. Sci. Environ. Technol., 3(4): 1328-1335.
 
2. Kumar, S. (2007) Commercial goat farming in India: An emerging agri-business opportunity. Agric. Econ. Res. Rev., 20: 503-520.
 
3. Nimbkar, C. and van Arendonk, J. (2010) Recent Trends in Global Organization of Animal Breeding "Paper for the International Technical Expert Workshop" Exploring the Need for Specific Measures for Access and Benefit Sharing of Animals Genetic Resource for Food and Agriculture. Wageningen, Netherlands, Dec. 8-10.
 
4. Mittal, P.K., Anand, M., Madan, A.K., Yadav, S. and Kumar, J. (2014) Antioxidative capacity of vitamin E, vitamin C and their combination in cryopreserved Bhadawari bull semen. Vet. World, 7(12): 1127-1131.
http://dx.doi.org/10.14202/vetworld.2014.1127-1131
 
5. Kathiravan, P., Kalatharan, J., Karthikeya, G., Rengarajan, K. and Kadirvel, G. (2011) Objective sperm motion analysis to assess dairy bull fertility using computer aided system – A review. Reprod. Domest. Anim., 46: 165-172.
http://dx.doi.org/10.1111/j.1439-0531.2010.01603.x
PMid:20403134
 
6. Mortimer, S.T. (2000) CASA-practical aspects. J. Androl., 21: 515-524.
PMid:10901437
 
7. Foote, R.H. (2003) Fertility estimation: A review of past experience and future prospects. Anim. Reprod. Sci., 75: 119-139.
http://dx.doi.org/10.1016/S0378-4320(02)00233-6
 
8. Dorado, J., Munoz-Serrano, A. and Hidalgo, M. (2010) The effect of cryopreservation on goat semen characteristics related to sperm freezability. Anim. Reprod. Sci., 121: 115-123.
http://dx.doi.org/10.1016/j.anireprosci.2010.04.182
PMid:20462708
 
9. Hancock, J.L. (1952) The morphology of bull spermatozoa. J. Exp. Biol., 29: 445-453.
 
10. Jeyendran, R.S., Vander-Ven, H.H., Perez-Pelaez, M., Crabo, B.G. and Zanevld, L.J.D. (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characters. J. Repord. Fertil., 70: 219-228.
http://dx.doi.org/10.1530/jrf.0.0700219
PMid:6694140
 
11. Watson, P.F. (1975) Use of giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet. Res., 97: 12-15.
http://dx.doi.org/10.1136/vr.97.1.12
 
12. Leboeuf, B., Restall, B. and Salomon, S. (2000) Production and storage of goat semen for artificial insemination. Anim. Reprod. Sci., 62: 113-141.
http://dx.doi.org/10.1016/S0378-4320(00)00156-1
 
13. Roy, A. (1957) Egg yolk-coagulation enzyme in the semen and Cowper's gland of the goat. Nature, 179: 318-319.
http://dx.doi.org/10.1038/179318b0
PMid:13407715
 
14. Gilmore, J.A., Du, J., Tao, J., Peter, A.T. and Critser, J.K. (1996) Osmotic properties of boar spermatozoa and their relevance to cryopreservation. J. Reprod. Fertil., 107: 87-95.
http://dx.doi.org/10.1530/jrf.0.1070087
PMid:8699439
 
15. Rodenas, C., Parrilla, I., Roca, J., Martinez, E.A. and Lucas, X. (2014) Effects of rapid cooling prior to freezing on the quality of canine cryopreserved spermatozoa. J. Reprod. Dev., 60(5): 355-361.
http://dx.doi.org/10.1262/jrd.2014-024
PMCid:PMC4219992
 
16. Oldenhof, H., Friedel, K., Akhoondi, M., Gojowsky, M., Wolkers, W.F. and Sieme, H. (2012) Membrane phase behavior during cooling of stallion sperm and its correlation with freezability. Mol. Membr. Biol., 29(4): 95-106.
http://dx.doi.org/10.3109/09687688.2012.674161
PMid:22480267
 
17. Mazur, P. (2010) A biologist's view of the relevance of thermodynamics and physical chemistry to cryobiology. Cryobiology, 60: 4-10.
http://dx.doi.org/10.1016/j.cryobiol.2009.12.001
PMid:19962974 PMCid:PMC3733660
 
18. Qureshi, M.S., Khan, D., Mushtaq, A. and Afridi, S.S. (2013) Effect of extenders, postdilution intervals, and seasons on semen quality in dairy goats. Turk. J. Vet. Anim. Sci., 37: 147-152.
 
19. Agarwal, A., Saleh, R.A. and Bedaiwy, M.A. (2003) Role of reactive oxygen species in the patho-physiology of human reproduction. Fertil. Steril., 79: 829-843.
http://dx.doi.org/10.1016/S0015-0282(02)04948-8
 
20. Zhu, H., Luo, H.L., Meng, H. and Zhang, G.J. (2009) Effect of vitamin E supplementation on development of reproductive organs in Boer goat. Anim. Reprod. Sci., 113: 93-101.
http://dx.doi.org/10.1016/j.anireprosci.2008.05.076
PMid:18597956
 
21. Blassea, A.K., Oldenhofb, H., Ekhlasi-Hundriesera, M., Wolkersc, W.F., Siemeb, H. and Bollweina, H. (2012) Osmotic tolerance and intracellular ion concentrations of bovine sperm are affected by cryopreservation. Theriogenology, 78: 1312-1320.
http://dx.doi.org/10.1016/j.theriogenology.2012.05.029
PMid:22819283
 
22. Amidi, F., Farshad, A. and Khor, A.K. (2010) Effects of cholesterol-loaded cyclodextrin during freezing step of cryopreservation with TCGY extender containing bovine serum albumin on quality of goat spermatozoa. Cryobiology, 61: 94-99.
http://dx.doi.org/10.1016/j.cryobiol.2010.05.006
PMid:20561514
 
23. Bezerra, F.S.B., Castelo, T.S., Alves, H.M., Oliveira, I.R.S., Lima, G.L., Gislayne, C.X., Peixoto, G.C.X., Bezerra, A.C.S. and Silva, A.R. (2011) Objective assessment of the cryoprotective effects of dimethylformamide for freezing goat semen. Cryobiology, 63: 263-266.
http://dx.doi.org/10.1016/j.cryobiol.2011.09.136
PMid:21985769
 
24. Alvarez, J.G. (2012) Male Infertility: Loss of Intracellular Antioxidative Enzymes Activity during Sperm Cryopreservation: Effect of Sperm Function after Thawing. Springer, New York. p237-244.
 
25. Munoz, O.V., Briand, L.A., Bancharif, D., Anton, M., Deserches, S., Shmitt, E., Thorin, C. and Tainturier, D. (2010) Effect of low density, spermatozoon concentration and glycerol on functional and motility parameter of Bull spermatozoa during storage at 4C. Asian J. Androl., 13(2): 281-286.
http://dx.doi.org/10.1038/aja.2010.84
PMid:21113174 PMCid:PMC3739216
 
26. Wongtawan, T., Saravia, F., Wallgren, M., Caballero, A. and Rodriguez-Martinez, H. (2006) Fertility after deep intra-uterine artificial insemination of concentrated low-volume boar semen doses. Theriogenology, 65: 773-787.
http://dx.doi.org/10.1016/j.theriogenology.2005.07.003
PMid:16085297