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Research (Published online: 27-02-2016)

19. Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers - Ashwani Kumar Roy, Mahendra Singh, Parveen Kumar and B. S. Bharath Kumar

Veterinary World, 9(2): 216-221



   doi: 10.14202/vetworld.2016.216-221



Ashwani Kumar Roy: Division of Dairy Cattle Physiology, National Dairy Research Institute, Karnal, Haryana, India;

Mahendra Singh: Division of Dairy Cattle Physiology, National Dairy Research Institute, Karnal, Haryana, India;

Parveen Kumar: Division of Dairy Cattle Physiology, National Dairy Research Institute, Karnal, Haryana, India;

B. S. Bharath Kumar: Division of Dairy Cattle Physiology, National Dairy Research Institute, Karnal, Haryana, India;


Received: 17-08-2015, Revised: 08-01-2016, Accepted: 16-01-2016, Published online: 27-02-2016


Corresponding author: Ashwani Kumar Roy, e-mail:

Citation: Roy AK, Singh M, Kumar P, Kumar BSB (2016) Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers, Veterinary World, 9(2): 216-221.

Aim: To investigate the effect of extended photoperiod on growth rate, hormonal levels, and puberty in Murrah heifers.

Materials and Methods: About 14 Murrah buffalo heifers were divided into normal day photoperiod (NDP; n=7) and extended NDP (ENDP; n=7) groups. The ENDP group was exposed to 4 h of extended photoperiod with artificial light (160 lux) after sunset for 3 months during winter.

Results: Group, age and group-by-age interaction effects on plasma glucose concentrations were non-significant (p>0.05). A significant effect of age on non-esterified fatty acids (p<0.05), cholesterol (p<0.01), and triglycerides (p<0.05) concentrations was observed. Group and group-by-age interaction effects on plasma T3, T4, leptin, 17 β estradiol, prolactin and melatonin concentrations were non-significant (p>0.05) while significant (p<0.05) age effect on T4, leptin and melatonin concentrations was observed. With respect to the circadian pattern of melatonin and prolactin, the group, time and groupby-time interaction effects were non-significant (p>0.05). Average daily gain and dry matter intake of heifers were nonsignificant between the NDP and ENDP groups but were comparatively higher in ENDP group. By the end of the experiment, 6 out of 7 heifers attained puberty in ENDP group in comparison to 4 out of 7 in NDP group.

Conclusion: Extending the photoperiod by artificial light for 4 h during winter season resulted in better growth rate and early onset of puberty in Murrah buffalo heifers.

Keywords: buffalo, leptin, melatonin, metabolites, photoperiod, prolactin, puberty.

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PMid:24996419 PMCid:PMC4094414