Open Access
Research
(Published
online:
08-02-2016)
3.
Isolation and characterization of Shiga toxigenic
Escherichia coli
of animal and bird origin by multiplex
polymerase chain reaction -
S. Neher, A. K. Hazarika, L. M. Barkalita, P. Borah, D. P. Bora
and R. K. Sharma
Veterinary World, 9(2): 123-127
doi:
10.14202/vetworld.2016.123-127
S. Neher:
Department of Veterinary Microbiology, College of Veterinary
Science, Assam Agricultural University, Guwahati, Assam, India;
samsunneher87@gmail.com
A. K. Hazarika:
Department of Veterinary Microbiology, College of Veterinary
Science, Assam Agricultural University, Guwahati, Assam, India;
hazarikaonline@gmail.com
L. M. Barkalita:
Department of Animal Biotechnology, College of Veterinary
Science, Assam Agricultural University, Guwahati, Assam, India;
luitbarkalita@gmail.com
P. Borah:
Department of Animal Biotechnology, College of Veterinary
Science, Assam Agricultural University, Guwahati, Assam, India;
borahp@vetbifguwahati.ernet.in
D. P. Bora:
Department of Veterinary Microbiology, College of Veterinary
Science, Assam Agricultural University, Guwahati, Assam, India;
drdpbora@gmail.com
R. K. Sharma:
Department of Veterinary Microbiology, College of Veterinary
Science, Assam Agricultural University, Guwahati, Assam, India;
dr.sharmark@rediffmail.com
Received: 20-08-2015, Revised: 17-12-2015, Accepted: 21-12-2015,
Published online: 08-02-2016
Corresponding author:
S. Neher, e-mail: samsunneher87@gmail.com
Citation:
Neher S, Hazarika AK, Barkalita LM, Borah P, Bora DP, Sharma RK
(2016) Isolation and characterization of Shiga toxigenic
Escherichia coli
of animal and bird origin by multiplex polymerase chain
reaction,
Veterinary World 9(2):
123-127.
Abstract
Aim:
The purpose of this study was to determine the virulence genes
and serotype of Shiga toxin producing
Escherichia coli
(STEC) strains isolated from animals and birds.
Materials and Methods:
A total of 226 different samples
viz.,
fecal, intestinal content, rectal swab and heart blood were
collected from different clinically affected/healthy animals and
birds and were streaked on McConkeys’ lactose agar and eosin
methylene blue agar for isolation of
E. coli,
confirmed by staining characteristics and biochemical tests. By
polymerase chain reaction (PCR) all the
E. coli
isolates were screened for certain virulence genes,
viz.,
Shiga toxin 1 (stx1),
stx2
and
eae
and enterohemolytic (Ehly) phenotype was observed in washed
sheep blood agar plate. All the isolated
E. coli
strains were forwarded to the National
Salmonella
and
Escherichia
Centre, Central Research Institute, Kasauli (Himachal Pradesh)
for serotyping.
Results:
Out of 226 samples 138 yielded
E. coli.
All the isolates were screened for molecular detection of
different virulent genes,
viz. stx1,
stx2
and
eae,
based on which 36 (26.08%) were identified as STEC. Among those
STEC isolates, 15 (41.67%), 14 (38.89%), 1 (2.78%) exhibited
eae, stx2, stx1
alone, respectively, whereas 4 (11.11%) and 2 (5.56%) carried
both
stx1
and
stx2, stx2
and
eae,
respectively. Among the STEC isolates 22 were belonged to 15
different sero-groups,
viz.,
O2, O20, O22, O25, O43, O60, O69, O90, O91, O95, O106, O118,
O130, O162 and O170 and others were untypable. Ehly phenotype
was observed in 10 (27.78%) the STEC isolates.
Conclusion:
The present study concluded that STEC could be isolated from
both clinically affected as well as healthy animals and birds.
Regular monitoring of more samples from animal and bird origin
is important to identify natural reservoir of STEC to prevent
zoonotic infection.
Keywords:
eae,
Escherichia coli,
Shiga toxigenic
Escherichia coli,
Shiga toxin 1, Shiga toxin 2.
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