| 
              
              
              Open Access  
 
              
              
              
              Research 
              
              
(Published 
				online: 
				13-02-2016)  
              8. 
				
				
				Effect of incorporation of calcium lactate 
				on physico-chemical, textural, and sensory properties of 
				restructured buffalo meat loaves - 
				
				A. Irshad, B. D. Sharma, S. R. Ahmed, S. Talukder, O. P. Malav 
				and Ashish Kumar 
              
              Veterinary World, 9(2): 151-159   
              
   
                
                
doi: 
              
				
				10.14202/vetworld.2016.151-159 
                
				  
				
				A. Irshad: 
				
				Department of Livestock Products Technology, College of 
				Veterinary and Animal Sciences, Kerala Veterinary and Animal 
				Sciences University, Mannuthy, Thrissur - 680 651, Kerala, 
				India; irshad2k6@gmail.com 
				
				B. D. Sharma: 
				
				Division of Livestock Products Technology, Indian Veterinary 
				Research Institute, Izatnagar - 243 122, Uttar Pradesh, India; 
				dr_bdsharma@yahoo.com 
				
				S. R. Ahmed: 
				
				Division of Livestock Products Technology, Indian Veterinary 
				Research Institute, Izatnagar - 243 122, Uttar Pradesh, India; 
				Sh.rafeh@gmail.com 
				
				S. Talukder: 
				
				Division of Livestock Products Technology, Indian Veterinary 
				Research Institute, Izatnagar - 243 122, Uttar Pradesh, India; 
				drttalukder@gmail.com 
				
				O. P. Malav: 
				
				Department of Livestock Products Technology, College of 
				Veterinary Science, Guru Angad Dev Veterinary and Animal 
				Sciences University, Ludhiana, Punjab, India; drmalav_vet2007@rediffmail.com 
				
				Ashish Kumar: 
				
				Division of Livestock Products Technology, Indian Veterinary 
				Research Institute, Izatnagar - 243 122, Uttar Pradesh, India; 
				ashish07.vet@gmail.com   
				
				Received: 10-06-2015, Revised: 25-12-2015, Accepted: 30-12-2015, 
				Published online: 13-02-2016 
				  
				
              	
              	Corresponding author:A. Irshad, e-mail: irshad2k6@gmail.com 
 
              Citation: 
				
				Irshad A, Sharma BD, Ahmed SR, Talukder S, Malav OP, Kumar A 
				(2016) Effect of incorporation of calcium lactate on physico-chemical, 
				textural, and sensory properties of restructured buffalo meat 
				loaves, 
				
				Veterinary World 9(2): 
				151-159. 
 
              
				Abstract 
 
				
				
				Aim: 
				
				The present study was conducted to develop a functional meat 
				product by fortifying calcium (in the form of calcium lactate) 
				with restructured buffalo meat loaf (RBML). 
				
				
				Materials and Methods: 
				
				Deboned buffalo meat obtained from the carcass of adult female 
				buffalo within 5-6 h of slaughter and stored under frozen 
				condition. Calcium fortified RBML were prepared by replacing the 
				lean buffalo meat with calcium lactate powder at 0%, 1%, 1.25%, 
				and 1.5% level through the pre-standardized procedure. The 
				developed products were evaluated for physico-chemical 
				properties, proximate composition, calcium concentration (mg/100 
				g), water activity (aw), Lovibond® tintometer color units, 
				texture profile analysis (TPA), and sensory qualities as 
				per-standard procedures. 
				
				
				Results: 
				
				Of the various product quality parameters evaluated, cooking 
				yield (%), product pH, moisture (%), protein (%), fat (%), and 
				water activity (aw) decreases significantly with increasing 
				level of calcium lactate. Calcium content of fortified 
				functional RBMLs was 135.02, 165.73, and 203.85 mg/100 g as 
				compared to 6.48 mg/100 g in control. Most of the sensory scores 
				at 1% and 1.25% levels of calcium lactate in treatment products 
				remained comparable among themselves and control product, with a 
				gradual decline. 
				
				
				Conclusions: 
				
				The present study concluded that 1.25% calcium lactate was the 
				optimum level for the fortification of calcium in RBML without 
				affecting the textural and sensory properties which could meet 
				out 15% of recommended dietary allowance for calcium. 
				
				Keywords: 
				
				buffalo meat, calcium fortification, Lovibond® tintometer color 
				units, meat loaves, restructured products, texture profile 
				analysis and sensory attributes. 
 
              References 
 
				
					| 1. Vavrusova, M. and Skibsted, L.H. (2014) Calcium 
					nutrition, bioavailability and fortification. Food Sci. 
					Technol., 59(2): 1198-1204. http://dx.doi.org/10.1016/j.lwt.2014.04.034
 |  
					|  |  
					| 2. Caceres, E., Garcya, M.L. and Selgas, M.D. (2006) Design 
					of a new cooked meat sausage enriched with calcium. Meat Sci., 
					73: 368-377. http://dx.doi.org/10.1016/j.meatsci.2005.12.016
 PMid:22062310
 |  
					|  |  
					| 3. Adluri, R.S., Zhan, L., Bagchi, M., Maulik, N. and Maulik, 
					G. (2010) Comparative effects of a novel plant-based calcium 
					supplement with two common calcium salts on proliferation 
					and mineralization in human osteoblast cells. Mol. Cell. 
					Biochem., 340(1-2): 73-80. http://dx.doi.org/10.1007/s11010-010-0402-0
 PMid:20213262
 |  
					|  |  
					| 4. Jimenez-Colmenero, F., Herrero, A., Cofrades, S. and 
					Capillas, C.R. (2012) Meat and functional foods. In: Hui, 
					Y.H., editor. Handbook of Meat and Meat Processing. 2nd ed. 
					CRC Press, Taylor & Francis Group, Boca Raton, London, New 
					York. p225-242. http://dx.doi.org/10.1201/b11479-15
 |  
					|  |  
					| 5. Jimenez-Colmenero, F., Sanchez-Muniz, F. and 
					Olmedilla-Alonso, B. (2010) Design and development of 
					meat-based functional foods with walnut: Technological, 
					nutritional and health impact. Food Chem., 123: 959-967. http://dx.doi.org/10.1016/j.foodchem.2010.05.104
 |  
					|  |  
					| 6. Institute of Medicine (IOM). (2010) Dietary Reference 
					Intakes: Recommended Intakes for Individuals. OIM, New York. |  
					|  |  
					| 7. Soto, A.M., Morales, P., Haza, A.I., Garcia, M.L. and 
					Selga, M.D. (2014) Bioavailability of calcium from enriched 
					meat products using Caco-2 cells. Food Res. Int., 55: 
					263-270. http://dx.doi.org/10.1016/j.foodres.2013.10.038
 |  
					|  |  
					| 8. Moseley, K. and de Beur, S. (2011) Principles of 
					Gender-Specific Medicine. Elsevier Academic Press, London, 
					UK. p716-736. |  
					|  |  
					| 9. Neilson, A. and Ferruzi, M. (2013) Nutrition in the 
					Prevention and Treatment of Disease. 3rd ed. Academic Press, 
					London, UK. |  
					|  |  
					| 10. Mesias, M., Seiquer, I. and Navarro, M.P. (2011) Calcium 
					nutrition in adolescence. Crit. Rev. Food Sci. Nutr., 51: 
					195-209. http://dx.doi.org/10.1080/10408390903502872
 PMid:21390941
 |  
					|  |  
					| 11. Aggarwal, P., Ahlawat, S.S. and Sharma, D.P. (2009) 
					Development of calcium enriched chicken meat rolls. Indian 
					J. Poultr. Sci., 44(2): 233-237. |  
					|  |  
					| 12. Brant, L.A. (2003) Formulation challenge: Calcium the 
					essential mineral, Issue December. Available from: 
					http://www.preparedfoods.com/articles/103615-formulation-challenge-calcium-the-essential-mineral. 
					Accessed on 03-06-2015. |  
					|  |  
					| 13. Kilgore, L.T., Watson, K., Wren, N., Rogers, R.W. and 
					Windham, F. (1977) Fortification of hamburger with calcium, 
					vitamin A and ascorbic acid. J. Am. Diet. Assoc., 71(2): 
					135-139. PMid:881532
 |  
					|  |  
					| 14. Boyle, E.A.E., Addis, P.B. and Epley, R.J. (1994) 
					Calcium fortified, reduced fat beef emulsion product. J. 
					Food Sci., 59(5): 928-932. http://dx.doi.org/10.1111/j.1365-2621.1994.tb08160.x
 |  
					|  |  
					| 15. Daengprok, W., Garnjanagoonchorn, W. and Mine, Y. (2002) 
					Fermented pork sausage fortified with commercial or hen egg 
					shell calcium lactate. Meat Sci., 62: 199-204. http://dx.doi.org/10.1016/S0309-1740(01)00247-9
 |  
					|  |  
					| 16. Vavrusova, M. and Skibsted, L.H. (2014) Spontaneous 
					super saturation of calcium D gluconate during isothermal 
					dissolution of calcium L-lactate in aqueous sodium 
					D-gluconate. Food Funct., 5: 85-91. http://dx.doi.org/10.1039/C3FO60205B
 PMid:24281051
 |  
					|  |  
					| 17. Vavrusova, M., Raitio, R., Orlien, V. and Skibsted, L.H. 
					(2013) Calcium hydroxy palmitate: Possible precursor phase 
					in calcium precipitation by palmitate. Food Chem., 138: 
					2415-2420. http://dx.doi.org/10.1016/j.foodchem.2012.12.012
 |  
					|  |  
					| 18. Cilla, A., Lagarda, M.J., Alegría, A., Ancos, B., Cano, 
					M.P., Sanchez-Moreno, C., Plaza, L. and Barberá, R. (2011) 
					Effect of processing and food matrix on calcium and 
					phosphorous bioavailability from milk-base fruit beverages 
					in Caco-2 cells. Food Res. Int., 44: 3030-3038. http://dx.doi.org/10.1016/j.foodres.2011.07.018
 |  
					|  |  
					| 19. Devatkal, S. and Mendiratta, S.K. (2001) Use of calcium 
					lactate with salt-phosphate and alginate-calcium gels in 
					restructured pork rolls. Meat Sci., 58: 371-379. http://dx.doi.org/10.1016/S0309-1740(01)00032-8
 |  
					|  |  
					| 20. Lawrence, T.E., Dikeman, M.E., Hunt, M.C., Kastner, C.L. 
					and Johnson, D.E. (2004) Effects of enhancing beef 
					longissimus with phosphate plus salt, or calcium lactate 
					plus non-phosphate water binders plus rosemary extract. Meat 
					Sci., 67: 129-137. http://dx.doi.org/10.1016/j.meatsci.2003.09.015
 PMid:22061126
 |  
					|  |  
					| 21. FAO. (2012) Food and Agriculture Organization of the 
					United Nations. FAOSTAT, Rome. |  
					|  |  
					| 22. Suresh, A., Kavita, B. and Chaudhary, K.R. (2012) 
					India's meat export: Structure, composition and future 
					prospects. J. Anim. Sci., 82(7): 749-756. |  
					|  |  
					| 23. APEDA. (2014) Export of agro and processed food products 
					including meat and meat products. Agricultural and Processed 
					Food Products Export Development Authority. Ministry of 
					Commerce, Government of India. |  
					|  |  
					| 24. Kandeepan, G., Anjaneyulu, A.S.R., Kondaiah, N., 
					Mendiratta, S.K. and Lakshmanan, V. (2009) Effect of age and 
					gender on the processing characteristics of buffalo meat. 
					Meat Sci., 83: 10-14. http://dx.doi.org/10.1016/j.meatsci.2009.03.003
 PMid:20416787
 |  
					|  |  
					| 25. Naveena, B.M., Sen, A.R., Muthukumar, M., Babji, Y. and 
					Kondaiah, N. (2011) Effects of salt and ammonium hydroxide 
					on the quality of ground buffalo meat. Meat Sci., 87: 
					315-320. http://dx.doi.org/10.1016/j.meatsci.2010.11.004
 PMid:21145665
 |  
					|  |  
					| 26. Aberle, E.D., Forrest, J.C., David, E.G. and Edward, 
					W.M. (2012) Principles of Meat Science. 5rd ed. Kendall Hunt 
					Publishing Company, Dubuque, Iowa, USA. |  
					|  |  
					| 27. Gadekar, Y.P., Sharma, B.D., Shinde, A.K., Verma, A.K. 
					and Mendiratta, S.K. (2014) Effect of natural antioxidants 
					on the quality of cured, restructured goat meat product 
					during refrigerated storage (4 ± 1 °C). Small Rumin. Res., 
					119(1-3): 72-80. http://dx.doi.org/10.1016/j.smallrumres.2014.03.005
 |  
					|  |  
					| 28. Trout, E.S., Hunt, M.C., Johnson, D.E., Claus, J.R., 
					Kastner, C.L. and Kropt, D.H. (1992) Characteristics of low 
					fat ground beef containing texture modifying ingredients. J. 
					Food Sci., 57(1): 19-24. http://dx.doi.org/10.1111/j.1365-2621.1992.tb05415.x
 |  
					|  |  
					| 29. AOAC. (2012) Official Methods of Analysis of AOAC 
					International. 19th ed. Association of Official Analytical 
					Chemists, Washington, DC. |  
					|  |  
					| 30. Haque, N. and Lai, M. (1999) Gross energy estimation In: 
					Shastry, V.R.B., Kamra, D.N. and Pathak, N.N. editors. 
					Laboratory Manual of Animal Nutrition. Indian Veterinary 
					Research Institute, Izatnagar. p71. |  
					|  |  
					| 31. Berry, B.W. and Stiffler, D.M. (1981) Effect of electric 
					stimulator boiling temperature formulation and rate of 
					freezing on sensory cooking chemical and physical properties 
					of ground beef patties. J. Food Sci., 46: 1103-1106. http://dx.doi.org/10.1111/j.1365-2621.1981.tb03000.x
 |  
					|  |  
					| 32. Talpatra, S.K., Ray, S.C. and Sen, K.C. (1940) 
					Estimation of phosphorus, choline, calcium, magnesium, 
					sodium, potassium in feeding stuffs. J. Vet. Sci. Anim. 
					Husb., 10: 243-245. |  
					|  |  
					| 33. Little, A.C. (1975) Off on a tangent. J. Food Sci., 40: 
					410-412. http://dx.doi.org/10.1111/j.1365-2621.1975.tb02213.x
 |  
					|  |  
					| 34. Froehlich, D.A., Gullet, E.A. and Usborne, W.R. (1983) 
					Effect of nitrite and salt on the colour, flavour and 
					overall acceptability of ham. J. Food Sci., 48: 152-154. http://dx.doi.org/10.1111/j.1365-2621.1983.tb14811.x
 |  
					|  |  
					| 35. Bourne, M.C. (1978) Texture profile analysis. Food 
					Technol., 32(7): 62-72. |  
					|  |  
					| 36. Means, W.J. and Schmidt, G.R (1986). Algin/calcium gel 
					as a raw and cooked binder in restructured beef steaks. J. 
					Food Sci., 51: 60-65. http://dx.doi.org/10.1111/j.1365-2621.1986.tb10836.x
 |  
					|  |  
					| 37. Keeton, J.T. (1983) Effect of fat and NaCl/phosphate 
					levels on the chemical and sensory properties of pork 
					patties. J. Food Sci., 48: 878-881. http://dx.doi.org/10.1111/j.1365-2621.1983.tb14921.x
 |  
					|  |  
					| 38. Snedecor, G.W. and Cochran, W.G. (1994) Statistical 
					Methods. 1st ed. East West Press Pvt., Ltd., New Delhi. |  
					|  |  
					| 39. Dunkan, D.B. (1995) Multiple range and multiple F-tests. 
					Biometrics, 11: 1-42. http://dx.doi.org/10.2307/3001478
 |  
					|  |  
					| 40. Alahakoon, A.U., Bae, Y.S., Kim, H.J., Jung, S., 
					Jayasena, D.D., Yong, H.I., Kim, S.H. and Jo, C. (2013) The 
					effect of citrus and onion peel extracts, calcium lactate, 
					and phosvitin on microbial quality of seasoned chicken 
					breast meat. CNU J. Agric. Sci., 40(2): 131-137. http://dx.doi.org/10.7744/cnujas.2013.40.2.131
 |  
					|  |  
					| 41. Sofos, J.N. (1983) Effects of reduced salt (NaCl) levels 
					on sensory and instrumental evaluation of frankfurters. J. 
					Food Sci., 48: 1692-1695, 1699. |  
					|  |  
					| 42. Naveena, B.M., Sen, A.R., Muthukumar, M., 
					Vaithiyanathan, S. and Babji, Y. (2006) The effect of 
					lactates on the quality of microwave-cooked chicken patties 
					during storage. J. Food Sci., 71: 603-608. http://dx.doi.org/10.1111/j.1750-3841.2006.00178.x
 |  
					|  |  
					| 43. Talukder, S., Sharma, B.D., Mendiratta, S.K., Malav, 
					O.P., Sharma, H. and Gokulakrishnan, P. (2013) Development 
					and evaluation of extended restructured chicken meat block 
					incorporated with Colocasia (Colocasia esculenta) flour. J. 
					Food Proc. Technol., 4: 207. |  
					|  |  
					| 44. Sharma, H., Sharma, B.D., Mendiratta, S.K., Talukder, S. 
					and Ramasamy, G. (2013) Efficacy of flaxseed flour as bind 
					enhancing agent on the quality of extended restructured 
					mutton chops. Asian-Aust. J. Anim. Sci., 27(2): 247-255. |  
					|  |  
					| 45. Mehta, N. (2008) Studies on fortification of low fat 
					chicken meat patties with calcium, vitamin E and vitamin C. 
					Thesis, M.V.Sc. Deemed University, Indian Veterinary 
					Research Institute, Izatnagar, India. |  
					|  |  
					| 46. Verma, A.K., Sharma, B.D. and Banerjee, R. (2010) Effect 
					of sodium chloride replacement and apple pulp inclusion on 
					the physico-chemical, textural and sensory properties of low 
					fat chicken nuggets. Food Sci. Technol., 43: 715-719. http://dx.doi.org/10.1016/j.lwt.2009.12.006
 |  
					|  |  
					| 47. Ambadkar, R.K. (2002) Studies on the effect of lactates 
					on the quality of cooked salami from buffalo meat. Thesis, 
					Ph.D. Deemed University, Indian Veterinary Research 
					Institute, Izatnagar, India. |  
					|  |  
					| 48. Brewer, M.S., Mckeith, F., Martin, S.E., Dallnier, A.W. 
					and Meyer, J. (1991) Sodium lactate effects on shelf life, 
					sensory and physical characteristics of fresh pork sausage. 
					J. Food Sci., 56(5): 1176-1178. http://dx.doi.org/10.1111/j.1365-2621.1991.tb04727.x
 |  
					|  |  
					| 49. Got, F., Rouseel-Akrim, S., Bayle, M.C. and Culioli, J. 
					(1995) Effects of calcium lactate on tenderness and flavour 
					of beef. Viandes-et-Produits-Cames, 17(6): 328-330. |  |