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Research (Published online: 13-01-2016)

7. Effect of age, sex and physiological stages on hematological indices of Banni buffalo (Bubalus bubalis) - Mehul D. Patel, Abdul Lateef, Hemen Das, Ajay S. Patel, Ajay G. Patel and Axay B. Joshi

Veterinary World, 9(1): 38-42

 

 

   doi: 10.14202/vetworld.2016.38-42

 

Mehul D. Patel: Department of Physiology and Biochemistry, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar

Dantiwada Agricultural University, Sardarkrushinagar, Dantiwada-385506, Gujarat, India; mehul.ptl1@gmail.com

Abdul Lateef: Department of Physiology and Biochemistry, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar

Dantiwada Agricultural University, Sardarkrushinagar, Dantiwada-385506, Gujarat, India; dr.alatif@live.com

Hemen Das: Department of Physiology and Biochemistry, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar

Dantiwada Agricultural University, Sardarkrushinagar, Dantiwada-385506, Gujarat, India; hemenvet@rediffmail.com

Ajay S. Patel: Department of Livestock Products Technology, College of Veterinary Science & Animal Husbandry, Anand Agricultural University, Anand- 388110, Gujarat, India; dr.ajaypatelvet2012@gmail.com

Ajay G. Patel: Department of Physiology and Biochemistry, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar

Dantiwada Agricultural University, Sardarkrushinagar, Dantiwada-385506, Gujarat, India; ajaypatel2341993@gmail.com

Axay B. Joshi: Department of Physiology and Biochemistry, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar

Dantiwada Agricultural University, Sardarkrushinagar, Dantiwada-385506, Gujarat, India; joshiaxay484@gmail.com

 

Received: 07-09-2015, Revised: 26-11-2015, Accepted: 04-12-2015, Published online: 13-01-2016

 

Corresponding author: Hemen Das, e-mail: hemenvet@rediffmail.com

Citation: Patel MD, Lateef A, Das H, Patel AS, Patel AG, Joshi AB (2016) Effect of age, sex and physiological stages on hemato logical indices of Banni buffalo (Bubalus bubalis), Veterinary World 9(1): 38-42.

Abstract

Aim: To determine the physiological baseline values for hematological indices of Banni buffalo Bubalus bubalis) as well as to assess their alteration due to age, sex and physiological stages.

Materials and Methods: A total of 42 clinically healthy Banni buffaloes were categorized into seven groups (n=6): Group I (male calves ≤1 year), Group II (bulls >1 year), Group III (female calves ≤1 year), Group IV (pregnant lactating buffaloes), Group V (non-pregnant lactating buffaloes), Group VI (pregnant dry buffaloes), and Group VII (non-pregnant dry buffaloes). Blood samples collected aseptically from all the experimental groups were analyzed employing automated hematology analyzer. The data obtained were statistically analyzed; the mean and standard deviations were calculated and set as the reference values.

Results: The erythrocytic indices viz. total erythrocytes count (TEC), hemoglobin, and packed cell volume (PCV) were significantly higher in bulls as compared to that of male calves unlike mean corpuscular volume, mean corpuscular hemoglobin (MCH), and MCH concentration. The female calves had higher TEC and PCV than the adult buffaloes irrespective of sex. The total leukocyte count (TLC) and neutrophil counts in male calves were significantly lower than the bulls unlike the eosinophil, while monocyte and basophil remained unchanged with age. The TLC, differential leukocyte count and platelet count varied non-significantly among the adult female groups at different physiological stages. However, neutrophils were found to be apparently higher in lactating buffaloes.

Conclusion: The present study would be helpful for physiological characterization of this unique buffalo breed of Gujarat. Further, data generated may be a tool for monitoring the health and prognosis as well as diagnosis of diseases.

Keywords: age, banni, erythrocytic, lactation, leukocytic indices, pregnancy, sex.

References

1. Mirzadeh, K., Tabatabaei,S., Bojarpour,M. and Mamoei,M. (2010)Comparative study of hematological parameters according to strain, age, sex, physiological status and season in Iranian cattle.J. Anim. Vet. Adv., 9: 2123-2127.
http://dx.doi.org/10.3923/javaa.2010.2123.2127
 
2. Zvorc, Z., Mrljak, V., Susic, V. and Gotal, J. (2006) Haematological and biochemical parameters during pregnancy and lactation in sows. Vet. Arch., 76:245-253.
 
3. Devi, R. and Kumar, M.P. (2012) Effect of ageing and sex on the ceruloplasmin (Cp) and the plasma protein levels. J. Clin. Diagn. Res., 6:577-580.
 
4. Cetin, N., Bekyurek, T. and Cetin, E. (2009) Effect of sex, pregnancy and season on some haematological and biochemical blood values in Angora rabbits. Scand. J. Lab. Anim. Sci., 36:155-162.
 
5. Yaqub, L.S., Kawu, M.U. and Ayo, J.O. (2013) Influence of reproductive cycle, sex, age and season on haematological parameters in domestic animals: A rev. J. Cell Anim. Biol.,7:37-43.
http://dx.doi.org/10.5897/JCAB12.063
 
6. Iriadam, M. (2007) Variation in certain haematological and biochemical parameters during the peri-partum period in Kilis does. Small Rumin. Res., 73:54-57.
http://dx.doi.org/10.1016/j.smallrumres.2006.11.001
 
7. GOI. (2006) 19th Livestock Census-2012; All India Report. Government of India.
 
8. Chaudhary, A.P. and Singh, K.P. (2012) Final Report of Banni Buffalo in Banni Area of Kachchh. In Situ Conservation Project on Banni Buffalo. (11th Five Year Plan Scheme, SDAU, Sardarkrushinagar).
 
9. Otto, F., Baggasse, P., Bogin, E., Harun, M. and Vilela, F. (2000) Biochemical blood profile of Angoni cattle in Mozambique. Israel Vet. Med. Assoc., 55: 1-9.
 
10. Snedecor, G.W. and Cochran, W.G. (1994) Statistical Method. 8thed. Oxford and IBH Publishing Co., New Delhi. p156-159.
 
11. Beechler, B.R., Jolles, A.E. and Ezenwa, V.O. (2009) Evaluation of hematologic values in free-ranging African buffalo (Syncerus caffer). J. Wildl Dis., 45:57-66.
http://dx.doi.org/10.7589/0090-3558-45.1.57
PMid:19204335
 
12. Uppal, G., Raina, V., Sharma, A., Anand, M., Kumar, R., Khaitan, B.K. and Grover, J.K. (2005) Use of simple haematological, biochemical and clinical parameters to monitor response of multiple myeloma patients on high dose thalidomide therapy. Indian J. Cancer, 42:46-50.
http://dx.doi.org/10.4103/0019-509X.15100
PMid:15805692
 
13. Mohan, N.H., Niyogi, D., Waghaye, J.Y. and Singh, H.N. (2009) Age-related hematological changes in Murrah buffalo (Bubalus bubalis) calves. Indian J. Anim. Sci., 79:369-371.
 
14. Paul, R.K., Gottam, G.S. and Pareek, S. (2011) Effect of lactation and pregnancy on serum biochemical and haematological profiles of Surti buffaloes. Vet. Pract., 12:94-96.
 
15. Chandra, N.K., Prasad, V.G.N.V., Narasimha-Reddy, C.H.E., Bhaskar, V., Pandiyan, G.D.V. and Muralinath, E. (2008)Hematology of graded Murrah buffaloes in the coastal region of Andhra Pradesh (India). Buffalo Bull., 27:236-239.
 
16. Haque, N., Ludri, A., Hossain, S.A. and Ashutosh, M. (2013) Impact on hematological parameters in young and adult Murrah buffaloes exposed to acute heat stress. Buffalo Bull., 32:321-326.
 
17. Ellah, M.R.A., Hamed, M.I.,Ibrahim, D.R. andRateb, H.Z. (2014) Serum biochemical and haematological reference intervals for water buffalo Bubalus bubalis heifers. J. S. Afr. Vet. Assoc.,85:962.
 
18. Leberbauer, C., Boulme, F., Unfried, G., Huber, J., Beug, H. and Mullner, E.W. (2005) Different steroids co-regulate long term expansion versus terminal differentiation in primary human erythroid progenitors. Blood, 105:85-94.
http://dx.doi.org/10.1182/blood-2004-03-1002
PMid:15358620
 
19. Silva, M.B., D'Angelino, J.L. and Araujo, W.P. (1992) Erythrogram evaluation of buffalo (Bubalus bubalis) created in the Valley region of Ribeira in São Paulo. Braz. J. Vet. Res. Anim. Sci.,29:113-119.
 
20. Khadjeh, G.H. and Papahn, A.A. (2002) Some hematological parameters in the Iranian (Khuzestan native) buffaloes. Indian J. Anim. Sci., 72:671-673.
 
21. Patil, M.D., Talvelkar, B.A., Joshi, V.G. and Deshmukh, B.T. (1992) Haematological studies in Murrah buffaloes. Indian Vet. J.,69:661-663.
 
22. Kumar, R., Sharma, I.J. and Quari, M.A. (2001) Status of haemogram, plasma proteins, minerals and electrolytes during anorexia and sub-clinical ketosis in cows and buffaloes. Indian J. Anim. Sci., 71:118-121.
 
23. Mbassa, G.K. and Poulsen, J.S. (1991) Influence of pregnancy, lactation and environment on haematological profiles in Danish landrace dairy goats (Capra hircus) of different parity. Comp. Biochem. Physiol. B.,100:403-412.
http://dx.doi.org/10.1016/0305-0491(91)90394-S
 
24. Plaschka, S.G. (1997) Parametros hematologicos y bioquímicos en el parto de la yegua de raza espanola. Med. Vet., 14:205-209.
 
25. Lurie, S. (1993) Changes in age distribution of erythrocytes during pregnancy: A longitudinal study. Gynecol. Obstet. Invest., 36:141-144.
http://dx.doi.org/10.1159/000292613
PMid:8244185
 
26. Kopp, R. and Hetesa, J. (2000) Changes of hematological indices of juvenite carp (Cyprinus carpio L.) under the influence of natural populations of cyanobacterial water blooms. Acta. Vet. Brno., 69:131-137.
http://dx.doi.org/10.2754/avb200069020131
 
27. Chineke, C.A., Ologun, A.G. and Ikeobi, C.O.N. (2006) Hematological parameters in rabbit breeds and crosses in humid tropics. Pak. J. Biol. Sci., 9:2102-2106.
http://dx.doi.org/10.3923/pjbs.2006.2102.2106
 
28. Randhawa, C.S., Pal, H., Randhawa, S.S. and Uppal, S.K. (2009) Influence of age, season, lactation on haematology and iron biochemistry of crossbred cattle. Indian J. Anim. Sci., 79:39-42.
 
29. Kaushalendra, C.H. (2012) Correlation between peripheral melatonin and general immune status of domestic goat, Capra hircus: A seasonal and sex dependent variation. Small. Rumin. Res., 107:147-156.
http://dx.doi.org/10.1016/j.smallrumres.2012.04.002
 
30. Ali, R. and Shukla, S.P. (2012) Haemato-biochemical changes in post-partum anoestrus buffaloes during low breeding season. Researcher, 4:55-58.
 
31. Das, H., Lateef, A., Panchasara, H.H., Parsani, H.R., Patel, J.B. and Shah, N.M. (2013) Profile of lactating Mehsani Buffalo (Bubalus bubalis). Technical Bulletin No. 04/2013. Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar.
 
32. Jacob, N. (2012) Haematological, biochemical and endocrine parameters at different ages and physiological stages in Gir cattle and Jaffarabadi buffaloes. Ph.D Thesis Submitted to Anand Agricultural University (AAU), Anand (Gujarat).
 
33. Shil, S.K., Shaikat, A.H., Nath, B.K., Khan, M.F.H. and Khan, S.A. (2012) Hemato-biochemical profile in lactating cows and their calves. J. Bangladesh Soc. Agric. Sci. Technol., 9: 41-44.
 
34. Canfield, P.J., Best, F.G., Fairburn, A.J., Purdie, J. and Gilham, M. (1984) Normal haematological and biochemical values for the swamp buffalo (Bubalus bubalis). Aust. Vet. J., 61:89-93.
http://dx.doi.org/10.1111/j.1751-0813.1984.tb15525.x
PMid:6743148
 
35. Prava, M., Dixit, N.K. and Tolankhomba, T.C. (2012) Leukocyte picture of Frieswal cattle. Indian Vet. J., 89:13-15.
 
36. Mikniene, Z., Maslauskas, K., Kerziene, S., Kucinskiene, J. and Kucinskas, A. (2014) The effect of age and gender on blood haematological and serum biochemical parameters in Zemaitkui horses. Vet. Med. Zoot.,65:37-43.