Open Access
Research
(Published
online: 22-07-2016)
13.
How safe is the use of
chlorpyrifos: Revelations through its effect on layer birds -
P. P. Singh, Ashok Kumar, R. S. Chauhan
and P. K. Pankaj
Veterinary World, 9(7): 753-758
doi:
10.14202/vetworld.2016.753-758
P. P. Singh :
Section of Animal
Science, KVK, Rajmata Vijayaraje Scindia Krishi Vishwavidyalaya,
Morena, Madhya Pradesh, India;
prabalsingh1978@gmail.com
Ashok Kumar :
Department of
Livestock Production and Management, College of Veterinary &
Animal Sciences, Govind Ballabh Pant University of Agriculture
and Technology, Pantnagar, Uttarakhand, India;
ashokakt@rediffmail.com
R. S. Chauhan :
Department of
Livestock Production and Management, College of Veterinary &
Animal Sciences, Govind Ballabh Pant University of Agriculture
and Technology, Pantnagar, Uttarakhand, India;
cpogbpuat@rediffmail.com
P. K. Pankaj :
Section of Transfer
of Technology, Livestock Production Management, ICAR-Central
Research Institute for Dryland Agriculture, Hyderabad, Telangana,
India; dr.pkpankaj@gmail.com
Received: 24-01-2016, Accepted: 10-05-2016, Published online:
22-07-2016
Corresponding author:
P. P.
Singh, e-mail: prabalsingh1978@gmail.com
Citation:
Singh PP, Kumar A, Chauhan RS, Pankaj PK (2016) How safe is the
use of chlorpyrifos: Revelations through its effect on layer
birds, Veterinary World, 9(7): 753-758.
Abstract
Aim:
The present
study was aimed to investigate the immunological competence of
chlorpyrifos (CPF) insecticide after oral administration in
layer chickens.
Materials and
Methods:
A total of 20
White Leghorn birds were given CPF in drinking water at 0.3 ppm/bird/day
(no observable effect level dose) for a period of 3-month.
Immune competence status of layer birds and chicks hatched from
CPF-treated birds were estimated at 15 days interval in layer
birds and monthly interval in chicks using immunological and
biochemical parameters.
Results:
There
was a significant decrease in values of total leukocytes count,
absolute lymphocyte count, absolute heterophil count, total
serum protein, serum albumin, serum globulin, and serum gamma
globulin in the birds treated with CPF as compared to control.
Similarly, immune competence tests such as lymphocyte
stimulation test, oxidative burst assay, and enzyme-linked
immunosorbent assay tests indicated lower immunity in birds
treated with CPF as compared to control. Subsequently, chicks
produced from CPF-treated birds were also examined for immune
competence, but no significant difference was observed between
chicks of both the groups.
Conclusion:
The
exposure to CPF produced hemo-biochemical and other changes that
could be correlated with changes in the immunological profile of
layer chickens suggesting total stoppage of using CPF in poultry
sheds.
Keywords:
blood biochemistry, chlorpyrifos, layers, immunity, organic
pollutant.
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