Open Access
Research
(Published
online: 23-07-2016)
14.
Testicular pathology, gonadal and
epididymal sperm reserves of Yankasa rams infected with
experimental Trypanosoma brucei brucei and
Trypanosoma evansi -
Yunusa A. Wada,
Sonnie J. Oniye,
Peter I. Rekwot
and Oluyinka O. Okubanjo
Veterinary World, 9(7): 759-765
doi:
10.14202/vetworld.2016.759-765
Yunusa A. Wada :
Department of
Biological Sciences, Faculty of Science, Ahmadu Bello
University, Zaria, Nigeria;
yunuwad@yahoo.com
Sonnie J. Oniye :
Department of
Biological Sciences, Faculty of Science, Ahmadu Bello
University, Zaria, Nigeria;
sjoniye@yahoo.com
Peter I. Rekwot :
National Animal
Production Research Institute, Ahmadu Bello University, P.M.B.
1096 Shika, Zaria, Nigeria;
bankwa2006@yahoo.com
Oluyinka O.
Okubanjo :
Department of
Veterinary Parasitology and Entomology, Faculty of Veterinary
Medicine, Ahmadu Bello University, Zaria, Nigeria;
sokubanjo2002@yahoo.com
Received: 17-12-2015, Accepted: 09-06-2016, Published:
23-07-2016
Corresponding author:
Yunusa A.
Wada, e-mail: yunuwad@yahoo.com
Citation:
Wada YA, Oniye SJ, Rekwot PI, Okubanjo OO (2016) Testicular
pathology, gonadal and epididymal sperm reserves of Yankasa rams
infected with experimental Trypanosoma brucei brucei and
Trypanosoma evansi, Veterinary World, 9(7):
759-765.
Abstract
Aim:
The study
was conducted to evaluate the pathological effects of
trypanosomosis on the testes, gonadal, and epididymal sperm
reserves of Yankasa rams for 98 days.
Materials and
Methods:
A total of 16
Yankasa rams, aged between 24 and 30 months and weighed between
22 and 25 kg, were acclimatized for a period of 2-months in a
clean fly proof house and were adequately fed and given water
ad-libitum. Of the 16 rams, 12 that were clinically fit for
the experiment at the end of the acclimatization period were
randomly divided into four groups: Groups I, II, III, and IV,
each having 3 rams. Groups I and II were each challenged singly
with experimental Trypanosoma brucei brucei (Federer
strain) and Trypanosoma evansi (Sokoto strain),
respectively, while Group III was challenged with mixed T.
brucei brucei and T. evansi parasites (50% of each
species in the infective inoculum) and Group IV was left as an
uninfected control. Each infected ram received 2 mL of the
infected blood containing 2×106
trypomastigotes via the jugular vein, while the
control group received 2 mL each, normal saline.
Results:
All
the infected rams developed clinical signs typical of
trypanosomosis at varying pre-patent periods. The gross lesions
observed in the infected rams in Group II were moderate and more
severe in those of Groups I and III. Histological sections of
the testes of infected rams (Groups I, II, and III) showed
moderate (T. evansi-infected group) to severe (mixed and
T. brucei brucei-infected groups) testicular
degenerations with reduction in number of spermatogenic cell
layers, degenerated seminiferous tubules, congested interlobular
spaces, loss of tissue architecture with significant (p<0.01)
depletion, and loss of gonadal and epididymal sperm reserves in
Groups I and III in comparison to Group II and the control Group
IV. No observable clinical signs and histopathological lesions
were found in those rams of the control Group IV.
Conclusion:
The
study concluded that trypanosomosis due to experimental T.
brucei brucei or T. evansi or mixed infections (of
both parasites) caused testicular damage, decreased epididymal
and gonadal sperm reserves and an important cause of infertility
in Yankasa rams.
Keywords:
gonadal sperm reserve, mixed infection, testicular degeneration,
Trypanosoma brucei brucei, Trypanosoma evansi,
Yankasa ram.
References
1. Soudre, A., Ouedraogo-Kone, S., Wurzinger, M., Muller,
S., Hanotte, O., Ouedraogo, A.G. and Solkner, J. (2013)
Trypanosomosis: A priority disease in tsetse challenged
areas of Burkina Faso. Trop. Anim. Health Prod., 45(2):
497-503.
http://dx.doi.org/10.1007/s11250-012-0248-4
PMid:23108586 |
|
|
2. Batista, J.S., Rodrigues, C.M.F., Olinda, R.G., Silva,
T.M., Vale, R.G., Câmara, A.C., Reboucas, R.E., Bezerra, F.S.,
García, H.A. and Teixeira, M.M.G. (2012). Highly
debilitating natural Trypanosoma vivax infections in
Brazilian calves: Epidemiology, pathology and probable
transplacental transmission. Parasitol. Res., 110: 73-80.
http://dx.doi.org/10.1007/s00436-011-2452-y
PMid:21626156 |
|
|
3. Silva, T.M., Olinda, R.G., Rodrigues, C.M., Camara, A.C.,
Lopes, F.C., Coelho, W.A., Ribeiro, M.F., Freitas, C.,
Teixeira, M.M. and Batista, J.S. (2013) Pathogenesis of
reproductive failure induced by Trypanosoma vivax in
experimentally infected pregnant ewes. Vet. Res., 44(1):
1-5.
http://dx.doi.org/10.1186/1297-9716-44-1
PMid:23289625 PMCid:PMC3598889 |
|
|
4. Mbaya, A.W., Nwosu, C.O. and Kumshe, H.A. (2011) Genital
lesions in male red fronted gazelles (Gazella rufifrons)
experimentally infected with Trypanosoma brucei and the
effect of melarsamine hydrochloride (Cymelarsan®) and
diminazene aceturate (Berenil®) in their treatment.
Theriogenology, 16: 721-728.
http://dx.doi.org/10.1016/j.theriogenology.2011.04.004
PMid:21601916 |
|
|
5. Okubanjo, O.O., Sekoni, V.O., Ajanusi, O.J., Nok, A.J.
and Adeyeye, A.A. (2014) Testicular and epididymal pathology
in Yankasa rams experimentally infected with Trypanosoma
congolense. Asian Pac. J. Trop. Dis., 4(3): 185-189.
http://dx.doi.org/10.1016/S2222-1808(14)60502-8 |
|
|
6. Bezerre, F.S.B., Garcia, H.A., Alves, H.M., Oliveira,
I.R.S., Silva, A.E., Teixeira, M.M.G. and Batista, J.S.
(2008) Trypanosoma vivax in testicular and epidydimal
tissues of experimentally infected sheep. Pesq. Vet. Bras.,
28: 575-582.
http://dx.doi.org/10.1590/S0100-736X2008001200002 |
|
|
7. Ikede, B.O. and Akpavie, S.O. (1982) Delay in resolution
of trypanosome induced genital lesions in male rabbit
infected with Trypanosoma brucei and treated with Diminazene
aceturate. Res. Vet. Sci., 32: 374-376.
PMid:7100655 |
|
|
8. Omeke, B.C. and Igboeli, G. (2000) Disruption of
spermatogenesis in boars sub-clinically infected with
Trypanosoma brucei. Anim. Reprod. Sci., 63: 197-204.
http://dx.doi.org/10.1016/S0378-4320(00)00172-X |
|
|
9. Sekoni, V.O., Rekwot, P.I. and Bawa, E.K. (2004) The
effect of trypanosomosis on sperm morphology in Zebu x
Friesian crossbred bulls. Trop. Anim. Health Prod., 36:
55-64.
http://dx.doi.org/10.1023/B:TROP.0000009528.91525.01
PMid:14979559 |
|
|
10. Sekoni, V.O. (1992) Effect of Trypanosoma vivax
infection on semen characteristics of Yankasa rams. Br. Vet.
J., 148: 501-506.
http://dx.doi.org/10.1016/0007-1935(92)90005-L |
|
|
11. Wilson, R.T. and Durkin, J.W. (1984) Age at permanent
incisor eruption in indigenous goats and sheep in semi-arid
Africa. Small Ruminant and Camel Group, Group Document No.
SR C3. International Livestock Centre for Africa, Addis
Ababa. p451-455.
http://dx.doi.org/10.1016/0301-6226(84)90056-3 |
|
|
12. Woo, P.T.K. (1969) The haematocrit centrifuge technique
for the detection of trypanosomes in blood. Can. J. Zool.,
47: 921-923.
http://dx.doi.org/10.1139/z69-150
PMid:5343381 |
|
|
13. Biryomumaisho, S., Rwakishaya, E.K., Melville, S.E.,
Cailleau, A. and Lubega, G.W. (2013) Livestock
trypanosomosis in Uganda: Parasite heterogeneity and anaemia
status of naturally infected cattle, goats and pigs.
Parasitol. Res., 112(4): 1443-1450.
http://dx.doi.org/10.1007/s00436-013-3275-9
PMid:23344247 |
|
|
|
14. Hoare, C.A. (1972) Trypanosomes of Mammals: A Zoological
Monograph. Blackwell Scientific Publications, Oxford and
Edinburgh. p1-3. |
|
|
15. Herbert, W.J. and Lumsden, W.H.R. (1976) Trypanosoma
brucei: A rapid "matching" method for estimation of the host
parasitaemia. Exp. Parasitol., 40(3): 427-431.
http://dx.doi.org/10.1016/0014-4894(76)90110-7 |
|
|
16. Rekwot, P.I., Oyedipe, E.O. and Ehoche, G.W. (1994) The
effects of feed restriction and realimentation on the growth
and reproductive function of Bokoloji bulls. Theriogenology,
42: 287-295.
http://dx.doi.org/10.1016/0093-691X(94)90273-9 |
|
|
|
17. Ogunlade, J.T., Ewuola, E.O., Gbore, F.A., Bandyopadhyay,
R., Niezen, J. and Egbunike, G.N. (2006) Testicular and
epididymal sperm reserves of rabbits fed fumonisin
contaminated diets. World Appl. Sci. J., 1(1): 35-38. |
|
|
|
18. Coles, E.H. (1986) Veterinary Clinical Pathology. 4th
ed. WB. Sounders Company, Philadelphia, London, Toronto,
Mexico City Rio de Janeiro, Sydney, Tokyo Hong Kong. p486. |
|
|
|
19. Luna, L.G. (1968) Manual of Histologic Staining Methods
of the Armed Forces Institute of Pathology. 3rd ed.
McGraw-Hill Book Company, New York. |
|
|
20. Sekoni, V.O., Njoku, C.O., Kumi-Diaka, J. and Saror, D.I.
(1990) Pathological changes in male genitals of cattle
infected with Trypanosoma vivax and Trypanosoma congolense.
Br. Vet. J., 146: 175-180.
http://dx.doi.org/10.1016/0007-1935(90)90011-Q |
|
|
|
21. Steel, R.G. and Torrie, J.H. (1980) Principles and
Procedures of Statistics: An Approach. 2nd ed. McGraw-Hill
Book Co. Inc., New York. |
|
|
22. Adamu, S., Fatihu, M.Y., Useh, N.M., Mamman, M., Sekoni,
V.O. and Esievo, K.A.N. (2007) Sequential testicular and
epididymal damage in Zebu bulls experimentally infected with
Trypanosoma vivax. Vet. Parasitol., 43: 29-34.
http://dx.doi.org/10.1016/j.vetpar.2006.07.022
PMid:16935425 |
|
|
23. Anosa, V.O. and Isoun T.T. (1980) Further observations
of the testicular pathology in Trypanosoma vivax infection
in sheep and goats. Res. Vet. Sci., 28: 151-160.
PMid:7414061 |
|
