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Research (Published online: 26-07-2016)

15. Effect of oral administration of Bacillus coagulans B37 and Bacillus pumilus B9 strains on fecal coliforms, Lactobacillus and Bacillus spp. in rat animal model - Lopamudra Haldar and D. N. Gandhi

Veterinary World, 9(7): 766-772



   doi: 10.14202/vetworld.2016.766-772



Lopamudra Haldar: Department of Basic Sciences, Faculty of Science and Technology, ICFAI University, Kamalghat - 799 210, Tripura, India;

D. N. Gandhi: Dairy Microbiology Division, National Dairy Research Institute, Karnal - 132 001, Haryana, India; DNG:


Received: 18-03-2016, Accepted: 16-06-2016, Published: 26-07-2016


Corresponding author: Lopamudra Haldar, e-mail:

Citation: Haldar L, Gandhi DN (2016) Effect of oral administration of Bacillus coagulans B37 and Bacillus pumilus B9 strains on fecal coliforms, Lactobacillus and Bacillus spp. in rat animal model, Veterinary World, 9(7): 766-772.

Aim: To investigate the effect of oral administration of two Bacillus strains on fecal coliforms, Lactobacillus and Bacillus spp. in rat animal model.

Materials and Methods: An in vivo experiment was conducted for 49-day period on 36 adult male albino Wister rats divided equally into to four groups. After 7-day adaptation period, one group (T1) was fed on sterile skim milk along with basal diet for the next 28 days. Second (T2) and (T3) groups received spore biomass of Bacillus coagulans B37 and Bacillus pumilus B9, respectively, suspended in sterilized skim milk at 8-9 log colony-forming units/ml plus basal diet for 28 days, while control group (T4) was supplied with clean water along with basal diet. There was a 14-day post-treatment period. A total of 288 fecal samples (8 fecal collections per rat) were collected at every 7-day interval starting from 0 to 49 days and subjected to the enumeration of the counts of coliforms and lactobacilli and Bacillus spores using respective agar media. In vitro acid and bile tolerance tests on both the strains were performed.

Results: The rats those (T2 and T3) received either B. coagulans B37 or B. pumilus B9 spore along with non-fermented skim milk showed decrease (p<0.01) in fecal coliform counts and increase (p<0.05) in both fecal lactobacilli and Bacillus spore counts as compared to the control group (T4) and the group fed only skim milk (T1). In vitro study indicated that both the strains were found to survive at pH 2.0 and 3.0 even up to 3 h and tolerate bile up to 2.0% concentration even after 12 h of exposure.

Conclusions: This study revealed that oral administration of either B. coagulans B37 or B. pumilus B9 strains might be useful in reducing coliform counts accompanied by concurrent increase in lactobacilli counts in the intestinal flora in rats.

Keywords: acid salt tolerance, antibacterial activity, Bacillus coagulans, Bacillus pumilus, bile salt tolerance, probiotics.

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