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Research (Published online: 30-07-2016)

19. Characterization of the atypical lymphocytes in African swine fever - Z. A. Karalyan, Z. R. Ter-Pogossyan, L. O. Abroyan, L. H. Hakobyan, A. S. Avetisyan, N. Yu Karalyan and E. M. Karalova

Veterinary World, 9(7): 792-800

 

 

   doi: 10.14202/vetworld.2016.792-800

 

 

Z. A. Karalyan: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS, 7 Hasratyan St, 0014 Yerevan, Armenia; zkaralyan@yahoo.com

Z. R. Ter-Pogossyan: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS, 7 Hasratyan St, 0014 Yerevan, Armenia; zaratp@gmail.com

L. O. Abroyan: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS, 7 Hasratyan St, 0014 Yerevan, Armenia; pbzhikyan@yahoo.gr

L. H. Hakobyan: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS, 7 Hasratyan St, 0014 Yerevan, Armenia; lina.hakobyan@gmail.com

A. S. Avetisyan: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS, 7 Hasratyan St, 0014 Yerevan, Armenia; a.avetis@mail.ru

Karalyan N. Yu: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS, 7 Hasratyan St, 0014 Yerevan, Armenia; naira_karalyan@mail.ru

E. M. Karalova: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS, 7 Hasratyan St, 0014 Yerevan, Armenia; z_karalyan@mb.sci.am

 

Received: 28-04-2016, Accepted: 23-06-2016, Published online: 30-07-2016

 

Corresponding author: Z. A. Karalyan, e-mail: zkaralyan@yahoo.com


Citation: Karalyan ZA, Ter-Pogossyan ZR, Abroyan LO, Hakobyan LH, Avetisyan AS, Karalyan NY, Karalova EM (2016) Characterization of the atypical lymphocytes in African swine fever, Veterinary World, 9(7): 792-800.



Aim: Atypical lymphocytes usually described as lymphocytes with altered shape, increased DNA amount, and larger size. For analysis of cause of genesis and source of atypical lymphocytes during African swine fever virus (ASFV) infection, bone marrow, peripheral blood, and in vitro model were investigated.

Materials and Methods: Atypical lymphocytes under the influence of ASFV were studied for morphologic, cytophotometric, and membrane surface marker characteristics and were used in vivo and in vitro models.

Results: This study indicated the increased size, high metabolic activity, and the presence of additional DNA amount in atypical lymphocytes caused by ASFV infection. Furthermore, in atypical lymphocytes, nuclear-cytoplasmic ratio usually decreased, compared to normal lymphocytes. In morphology, they looking like lymphocytes transformed into blasts by exposure to mitogens or antigens in vitro. They vary in morphologic detail, but most of them are CD2 positive.

Conclusions: Our data suggest that atypical lymphocytes may represent an unusual and specific cellular response to ASFV infection.

Keywords: African swine fever virus, atypical lymphocytes, CD2, DNA ploidy.



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