Veterinary World

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Research (Published online: 02-07-2016)

2. Evaluation of edible polymer coatings enriched with green tea extract on quality of chicken nuggets - Prathyusha Kristam, Naga Mallika Eswarapragada, Eswara Rao Bandi and Srinivas Rao Tumati

Veterinary World, 9(7): 685-692



   doi: 10.14202/vetworld.2016.685-692



Prathyusha Kristam: Department of Livestock Products Technology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India;

Naga Mallika Eswarapragada: Department of Livestock Products Technology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India;

Eswara Rao Bandi: Department of Livestock Products Technology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India;

Srinivas Rao Tumati: Department of Veterinary Public Health & Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India;


Received: 21-02-2016, Accepted: 25-05-2016, Published online: 02-07-2016


Corresponding author: Prathyusha Kristam, e-mail:

Citation: Kristam P, Eswarapragada NM, Bandi ER, Tumati SR (2016) Evaluation of edible polymer coatings enriched with green tea extract on quality of chicken nuggets, Veterinary World, 9(7): 685-692.

Aim: The present study was conducted to evaluate the physico-chemical and microbiological characteristics of chicken nuggets coated with sodium alginate (SA) coatings at refrigerated (4±1°C) and frozen (−18±1°C) storage condition at regular periodic intervals.

Materials and Methods: Chicken meat nuggets were separated into three groups: Uncoated control (C), coated with alginate coating (T1), and coated with alginate coating incorporated with 1% green tea extract (GTE) (T2). The nuggets were analyzed at regular intervals of 5days for refrigerated storage and 15 days for frozen storage period in terms of pH, 2-thiobarbituric acid value (TBA), peroxide value (PV), total plate count (TPC), water loss, and sensory characteristics.

Results: The results indicated that the nuggets coated with alginate-based coatings effectively reduced the spoilage as indicated by pH, TBA, and PVs. pH values of the formulations ranged from 6.15 to 6.34 at refrigerated storage temperature (4±1°C) and 6.49-6.71 at frozen storage temperature (−18±1°C). TBA value of the treatments ranged from 1.28 to 1.54 mg MDA/kg and 1.34 to 1.50 mg MDA/kg under refrigerated and frozen storage temperatures, respectively. Color, flavor, juiciness, tenderness, and overall acceptability of the nuggets differed significantly (p<0.05) with the coated nuggets. The coated nuggets were well acceptable upto 15 days at refrigerated storage temperature (4±1°C) and upto 75 days at frozen storage temperature (−18±1°C). Nuggets coated with GTE incorporated coating solution had a lower TBA-reactive substances values, PVs, and TPCs when compared to the nuggets coated with SA and the control group.

Conclusion: Study revealed that incorporation of edible coatings with antioxidants, namely, GTE at 1% level had a significant effect in reducing the fat oxidation. The samples recorded a shelf life of 15 days under refrigerated storage when compared to their controls with 10 days of storage period and 75 days under frozen storage against controls with 60 days. T1, T2, and T3 formulations had higher sensory scores in comparison to the controls. Overall acceptability scores of T1 were higher when compared to the other formulations.

Keywords: chicken nuggets, edible coatings, green tea extract, sodium alginate.

1. Ferrari, C.C., Sarantópoulos, C.I.G.,Carmello-Guerreiro, S.M. and Hubinger, M.D. (2011) Effect of osmotic dehydration and pectin edible coatings on quality and shelf life of fresh-cut melon.Food Bioprocess Technol., 60(1): 8091.
2. Moreiraa, M.R., Rouraa, S.I. andPoncea, S. (2011) Effectiveness of chitosan edible coatings to improve microbiological and sensory quality of fresh cut broccoli. Food Sci. Technol.Int.,44(10): 2335-2341.
3. Elena, P., Roi, R., Danino, S., Batia, H. and Victor, R. (2014) Gelatin-chitosan composite films and edible coatings to enhance the quality of food products layer-by-layer vs. Blended formulations. Food Bioprocess Technol., 7(1) 3319-3327
4. Mohana, C.O., Ravishankara, C.N., Lalitha, K.V. and Gopal, T.K.S. (2012) Effect of chitosan edible coating on the quality of double filleted Indian oil sardine (Sardinella longiceps) during chilled storage. Food Hydrocoll.,26(1):167-174.
5. Petrou, S., Tsiraki, M., Giatrakou, V. and Savvaidis, I.N.(2012) Chitosan dipping or oregano oil treatments, singly or combined on modified atmosphere packaged chicken breast meat.Food Microbiol., 156(3):264-271.
6. McHugh, T.H. and Avina-Bustillos, R.J.(2012) Applications of edible films and coatings to processed foods. In: Baldwin, E.A., Hagenmaier, R. and Bai, J., editors. Edible Coatings and Films to Improve Food Quality. CRC Press, Boca Raton. p291-318.
7. Kanatt, S.R., Rao, M.S.,Chawla, S.P. and Arun, S. (2012) Active chitosanepolyvinyl alcohol films with natural extracts. Food Hydrocoll., 29: 290-297.
8. López de Lacey, A.M., López-Caballero, M.E. and Montero, P. (2014) Agar films containing green tea extract and probiotic bacteria for extending fish shelf-life. Food Sci. Technol.,55:559-564.
9. Mehran, M., Hossein, T., Seyed, M., Razavi, R., Abdul, R., Oromiehiec, H.M., Javad, A. and Mojtaba, H.(2012) Characterization of antioxidant chitosan film incorporated with Zataria multiflora Boiss essential oil and grape seed extract. Food Sci. Technol., 46(2): 477-484.
10. Rhim, J.W. (2004) Physical and mechanical properties of water resistant sodium alginate films. Food Sci. Technol. Int., 37(3): 323-330.
11. Varela, P. and Fiszman, S.M. (2011) Hydrocolloids in fried foods. A review. Food Hydrocoll., 25(8): 1801-1812.
12. Benavidesa, S., Villalobos-Carvajalb, R. and Reyesb, J.E. (2012) Physical, mechanical and antibacterial properties of alginate film: Effect of the crosslinking degree and oregano essential oil concentration. J. Food Eng.,110(2): 232-239.
13. Kima, H.S., Quonc, M.J. and Kima, J.A. (2014) New insights into the mechanisms of polyphenols beyond antioxidant properties; Lessons from the green tea polyphenol, epigallocatechin 3-gallate. Redox. Biol., 2: 187-195.
PMid:24494192 PMCid:PMC3909779
14. Chen, L., Xin, X., Yuan, Q., Su, D. and Liu, W. (2013) Phytochemical properties and antioxidant capacities of various colored berries. J. Sci. Food Agric., 94(2):180-188
15. Chaturvedula, V.S.P. and Prakash, I. (2011) The aroma, taste, color and bioactive constituents of tea. J. Med. Plant Res., 5: 2110-2124.
16. Pripdeevech, P. and Machan, T. (2011) Fingerprint of volatile flavour constituents and antioxidant activities of teas from Thailand. Food Chem., 125: 797-802.
17. AOAC.(1995) Official Methods of Analysis. 6thed. Association Official Analytical Chemists International. Maryland, USA.
18. Trout, E.S., Hunt, N.C.,Jhonson, D.E., Claus, J.R.,Kastner, C.L. and Kropf, D.H. (1992) Chemical, physical and sensory characterization of ground beef containing 5 to 30% fat. J. Food Sci., 57:25-29.
19. Lawlor, J.B., Sheehy, P.J.A., Kerry, J.P., Buckley, D.J. and Morrisey, P.A. (2000) Measuring oxidative stability of beef muscles obtained from animals supplemented with vitamin E Using conventional and derivative spectrophotometry. J. Food Sci., 65(6): 1138-1141.
20. APHA. (1984) Compendium of Methods for the Microbiological Examination of Foods. 2nd ed. American Public Health Association, Washington, DC,USA.
21. Lu, F., Liu, D.H. and Ye, X.Q. (2009) Alginatee calcium coating incorporating nisin and EDTA maintains the quality of fresh northern snakehead (Channa argus) fillets stored at 4⁰C. J. Sci. Food Agric.,89: 848-854.
22. Chidanandaiah Keshri, R.C. and Sanyal, M.K. (2009) Effect of sodium alginate coating with preservatives on the quality of meat patties during refrigerated (4 ± 1⁰C) storage. J. Muscle Foods, 20: 275-292.
23. Yongling, S., Lei, L., Huixing, S., Juan, Y. and Yongkang, L. (2011) Effect of sodium alginate-based edible coating containing different anti-oxidants on quality and shelf life of refrigerated bream (Megalobrama amblycephala). Food Control, 22: 608-615.
24. Perumalla, A.V.S. and Hettiarachchy, N.S.(2011) Green tea and grape seed extracts e potential applications in food safety and quality. Food Res. Int., 44: 827-839.
25. Wu, Y., Weller, C.L., Hamouz, F., Cuppett, S. and Schnepf, M. (2001) Moisture loss and lipid oxidation for precooked ground-beef patties packaged in edible starch-alginate-based composite films. J. Food Sci., 66: 486-493.
26. Lee, C.H., An, D.S.,Lee, S.C., Park, H.J. and Lee, D.S.(2004) A coating for use as an antimicrobial and antioxidative packaging material incorporating nisin and a-tocopherol. J. Food Eng., 62:323-329.
27. Shon, J. and Chin, K.B. (2008) Effect of whey protein coating on quality attributes of low-fat, aerobically packaged sausage during refrigerated storage. J. Food Sci., 73(6):469-475.
28. Jeon, Y.J., Kamil, J.Y.V. and Shahidi, F. (2002) Chitosan as an edible invisible film for quality preservation of herring and Atlantic cod. J. Agric. Food Chem., 50:5167-5178.
29. Shon, J., Eo, J. and Eun, J.B. (2010) Effect of soy protein isolate coating on quality attributes of cut raw han-woo (Korean cow) beef, aerobically packaged and held refrigerated. J. Food Qual., 33(1): 42-60.
30. Rostami, H., Motallebi, A.A., Khanipour, A.A., Soltani, M. and Khanedan, N. (2010) Effect of whey protein coating on physico-chemical properties of gutted Kilka during frozen storage. Iran. J. Fish. Sci., 9(3): 412-421.
31. Natrajan, N. and Sheldon, B.W.(2000) Inhibition of Salmonella on poultry skin Using protein and polysaccharide-based films containing nisin formulation. J. Food Prot., 63: 1268-1272.
32. Cagri, A., Ustunol, Z. and Ryser, E. (2002) Inhibition of three pathogens on bologna and summer sausage slices using antimicrobial edible films. J. Food Sci., 67: 2317-2324.
33. Siripatrawan, U. and Noipha, S. (2012) Active film from chitosan incorporating green tea extract for shelf life extension of pork sausages. Food Hydrocoll., 27(1):102-108.
34. Seifzadeh, M., (2007) Application of edible films in fish products packaging. J. World Aquac., 7:34-40.
35. Kester, J.J. and Fennema, O.R. (1986) Edible films and coatings: A review. Food Ttechnol. Chicago, 40(12):47-59.