Open Access
Research
(Published
online: 05-07-2016)
4.
Effectiveness of a recombinant human
follicle stimulating hormone on the ovarian follicles,
peripheral progesterone, estradiol-17β, and pregnancy rate of
dairy cows -
Mohamed Ali and Moustafa M. Zeitoun
Veterinary World, 9(7): 699-704
doi:
10.14202/vetworld.2016.699-704
Mohamed Ali:
Department of Animal Production and Breeding, Qassim University,
College of Agriculture and Veterinary Medicine, Buraidah 6622,
Saudi Arabia; mohamed0_9@yahoo.com
Moustafa M. Zeitoun:
Department of Animal Production and Breeding, Qassim University,
College of Agriculture and Veterinary Medicine, Buraidah 6622,
Saudi Arabia; mmzeitoun@yahoo.com
Received: 12-03-2016, Accepted: 02-06-2016, Published online:
05-07-2016
Corresponding author:
Mohamed Ali, e-mail: mohamed0_9@yahoo.com
Citation:
Ali M, Zeitoun MM (2016) Effectiveness of a recombinant human
follicle stimulating hormone on the ovarian follicles,
peripheral progesterone, estradiol-17β, and pregnancy rate of
dairy cows, Veterinary World, 9(7): 699-704.
Abstract
Aims:
This study aimed at elucidating the effects of recombinant human
follicle stimulating hormone (r-hFSH) on the ovarian follicular
dynamics, progesterone, estradiol-17β profiles, and pregnancy of
dairy cows.
Materials and Methods:
Three groups (G, n=5 cows) of multiparous dairy cows were used.
G1 (C) control cows were given controlled internal drug release
(CIDR) and prostaglandin F2α; G2 (L) cows were given low dose
(525 IU and G3 (H) cows were given high dose (1800 IU) of r-hFSH
on twice daily basis at the last 3 days before CIDR removal. All
cows were ultrasonically scanned for follicular growth and
dynamics, and blood samples were collected every other day for
two consecutive estrus cycles for the determination of
estradiol-17β and progesterone.
Results:
Estrus was observed in all C and L but not in H cows. Dominant
follicle was bigger in L compared to C and H cows. Dominant
follicle in C (16.00±2.5 mm) and L cows (17.40±2.3 mm)
disappeared at 72 h after CIDR removal. However, in H cows, no
ovulation has occurred during 7 days post-CIDR removal.
Progesterone was not different (p>0.10) among groups, whereas
estradiol-17β revealed significant (p<0.01) reduction in H
(15.96±2.5 pg/ml) cows compared to C (112.26±26.1 pg/ml) and L
(97.49±15.9 pg/ml) cows. Pregnancy rate was higher in L cows
(60%) compared with C cows (20%). However, H cows were not
artificially inseminated due to non-ovulation. Only a cow of C
group has calved one calf, however, 2 of the L cows gave birth
of twins and a cow gave single calf.
Conclusion:
Administration of a low dose (525 IU) of r-hFSH resulted in an
optimal size of dominant follicle, normal values of progesterone
and estradiol-17β, and 40% twinning rate, howeverusing 1800 IU
of r-hFSH, have adverse effects on ovarian follicular dynamics
and hormonal profiles with non-pregnancy of dairy cows raised
under hot climate.
Keywords:
dairy cows, estradiol-17β, follicles, progesterone, recombinant
human follicle stimulating hormone.
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