Open Access
Research
(Published
online: 19-06-2016)
12.
Human umbilical mesenchymal stem cells
conditioned medium promote primary wound healing regeneration -
Dwi Liliek Kusindarta, Hevi Wihadmadyatami, Yuda Heru Fibrianto,
Widagdo Sri Nugroho, Heru Susetya, Dewi Kania Musana, Hery
Wijayanto, Surya Agus Prihatna and A. E. T. H. Wahyuni
Veterinary World, 9(6): 605-610
doi:
10.14202/vetworld.2016.605-610
Dwi Liliek Kusindarta:
Department of Anatomy, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; indarta@ugm.ac.id
Hevi Wihadmadyatami:
Department of Anatomy, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia;
heviwihadmadyatami@ugm.ac.id
Yuda Heru Fibrianto:
Department of Physiology, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; fibrianto1802@gmail.com
Widagdo Sri Nugroho:
Department of Veterinary Public Health, Faculty of Veterinary
Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;
weesnugroho@ugm.ac.id
Heru Susetya:
Department of Veterinary Public Health, Faculty of Veterinary
Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;
heruanggikiki@yahoo.com
Dewi Kania Musana:
Department of Anatomy, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; musanakd@ugm.ac.id
Hery Wijayanto:
Department of Anatomy, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; herykh@ugm.ac.id
Surya Agus Prihatna:
Department of Obstetrics and Gynecology, Faculty of Veterinary
Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;
prihatno@ugm.ac.id
A. E. T. H. Wahyuni:
Department of Microbiology, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Yogyakarta, Indonesia; wahyuni_aeth@yahoo.com
Received: 13-12-2015, Accepted: 06-05-2016, Published online:
19-06-2016
Corresponding author:
Dwi Liliek Kusindarta, e-mail: indarta@ugm.ac.id
Citation:
Kusindarta DL, Wihadmadyatami H, Fibrianto YH, Nugroho WS,
Susetya H, Musana DK, Wijayanto H, Prihatna SA, Wahyuni AETH
(2016) Human umbilical mesenchymal stem cells conditioned medium
promote primary wound healing regeneration,
Veterinary World, 9(6):
605-610.
Abstract
Aim:
This research was conducted to clarify the capability of human
umbilical mesenchymal stem cells conditioned medium (HU-MSCM) to
promote regenerations of primary wound healing on the incision
skin injury.
Materials and Methods:
In this study, two approaches
in vitro
and
in vivo
already done. On
in vitro
analysis, tube formation was performed using HU vein endothelial
cells in the presence of HU-MSCM, in some experiments cells line
was incubated prior the presence of lipopolysaccharide and
HU-MSCM then apoptosis assay was performed. Furthermore,
in vivo
experiments 12 female rats (Rattus
norvegicus)
were used after rats anesthetized, 7 mm wound was made by
incision on the left side of the body. The wound was treated
with HU-MSCM containing cream, povidone iodine was run as a
control. Wound healing regenerations on the skin samples were
visualized by hematoxylin-eosin staining.
Results:
In vitro
models elucidate HU-MSCM may decreasing inflammation at the
beginning of wound healing, promote cell migration and
angiogenesis. In addition
in vivo
models show that the incision length on the skin is decreasing
and more smaller, HE staining describe decreasing of
inflammation phase, increasing of angiogenesis, accelerate
fibroplasia, and maturation phase.
Conclusions:
Taken together our observation indicates that HU-MSCM could
promote the acceleration of skin tissue regenerations in primary
wound healing process.
Keywords:
human umbilical mesenchymal stem cells conditioned medium,
regenerations, wound healing.
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