Veterinary World

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Research (Published online: 19-06-2016)

12. Human umbilical mesenchymal stem cells conditioned medium promote primary wound healing regeneration - Dwi Liliek Kusindarta, Hevi Wihadmadyatami, Yuda Heru Fibrianto, Widagdo Sri Nugroho, Heru Susetya, Dewi Kania Musana, Hery Wijayanto, Surya Agus Prihatna and A. E. T. H. Wahyuni

Veterinary World, 9(6): 605-610



   doi: 10.14202/vetworld.2016.605-610



Dwi Liliek Kusindarta: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;

Hevi Wihadmadyatami: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;

Yuda Heru Fibrianto: Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;

Widagdo Sri Nugroho: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;

Heru Susetya: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;

Dewi Kania Musana: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;

Hery Wijayanto: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;

Surya Agus Prihatna: Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;

A. E. T. H. Wahyuni: Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia;


Received: 13-12-2015, Accepted: 06-05-2016, Published online: 19-06-2016


Corresponding author: Dwi Liliek Kusindarta, e-mail:

Citation: Kusindarta DL, Wihadmadyatami H, Fibrianto YH, Nugroho WS, Susetya H, Musana DK, Wijayanto H, Prihatna SA, Wahyuni AETH (2016) Human umbilical mesenchymal stem cells conditioned medium promote primary wound healing regeneration, Veterinary World, 9(6): 605-610.

Aim: This research was conducted to clarify the capability of human umbilical mesenchymal stem cells conditioned medium (HU-MSCM) to promote regenerations of primary wound healing on the incision skin injury.

Materials and Methods: In this study, two approaches in vitro and in vivo already done. On in vitro analysis, tube formation was performed using HU vein endothelial cells in the presence of HU-MSCM, in some experiments cells line was incubated prior the presence of lipopolysaccharide and HU-MSCM then apoptosis assay was performed. Furthermore, in vivo experiments 12 female rats (Rattus norvegicus) were used after rats anesthetized, 7 mm wound was made by incision on the left side of the body. The wound was treated with HU-MSCM containing cream, povidone iodine was run as a control. Wound healing regenerations on the skin samples were visualized by hematoxylin-eosin staining.

Results: In vitro models elucidate HU-MSCM may decreasing inflammation at the beginning of wound healing, promote cell migration and angiogenesis. In addition in vivo models show that the incision length on the skin is decreasing and more smaller, HE staining describe decreasing of inflammation phase, increasing of angiogenesis, accelerate fibroplasia, and maturation phase.

Conclusions: Taken together our observation indicates that HU-MSCM could promote the acceleration of skin tissue regenerations in primary wound healing process.

Keywords: human umbilical mesenchymal stem cells conditioned medium, regenerations, wound healing.

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