Open Access
Research
(Published
online: 19-06-2016)
13.
Evaluation of optimum roughage to concentrate ratio in maize
stover based complete rations for efficient microbial biomass
production using
in vitro
gas production technique -
Y. Ramana Reddy, N. Nalini Kumari, T. Monika and K. Sridhar
Veterinary World, 9(6): 611-615
doi:
10.14202/vetworld.2016.611-615
Y. Ramana Reddy:
Department of Animal Nutrition, College of Veterinary Science,
Kadapa - 516 360, Andhra Pradesh, India; ramanayr19@yahoo.co.in
N. Nalini Kumari:
Department of Animal Nutrition, College of Veterinary Science,
Hyderabad - 500 030, Telangana, India; nalini_reddy123@yahoo.co.in
T. Monika:
Department of Animal Nutrition, College of Veterinary Science,
Hyderabad - 500 030, Telangana, India; tmonika123@yahoo.co.in
K. Sridhar:
Department of Animal Nutrition, College of Veterinary Science,
Hyderabad - 500 030, Telangana, India; sri.vety@gmail.com
Received: 20-01-2016, Accepted: 10-05-2016, Published online:
19-06-2016
Corresponding author:
Y. Ramana Reddy, e-mail: ramanayr19@yahoo.co.in
Citation:
Ramana Reddy Y, Nalini Kumari N, Monika T, Sridhar K (2016)
Evaluation of optimum roughage to concentrate ratio in maize
stover based complete rations for efficient microbial biomass
production using
in vitro
gas production technique,
Veterinary World, 9(6):
611-615.
Abstract
Aim:
A study was undertaken to evaluate the optimum roughage to
concentrate ratio in maize stover (MS) based complete diets for
efficient microbial biomass production (EMBP) using
in vitro
gas production technique.
Materials and Methods:
MS based complete diets with roughage to concentrate ratio of
100:0, 90:10, 80:20, 70:30, 60:40, 50:50, 40:60, and 30:70 were
formulated, and 200 mg of oven-dried sample was incubated in
water bath at 39°C along with media (rumen liquor [RL] - buffer)
in
in vitro
gas syringes to evaluate the gas production. The gas produced
was recorded at 8 and 24 h of inc ubation.
In vitro
organic matter digestibility (IVOMD), metabolizable energy (ME),
truly digestible organic matter (TDOM), partitioning factor
(PF), and EMBP were calculated using appropriate formulae.
Ammonia nitrogen and total volatile fatty acids (TVFAs)
production were analyzed in RL fluid-media mixture after 24 h of
incubation.
Results:
In vitro
gas production (ml) at 24 h incubation, IVOMD, ME, TDOM, TVFA
concentration, and ammonia nitrogen production were increased
(p<0.01) in proportion to the increase in the level of
concentrate in the diet. Significantly (p<0.01) higher PF and
EMBP was noticed in total mixed ration with roughage to
concentrate ratio of 60:40 and 50:50 followed by 70:30 and
40:60.
Conclusion:
Based on the results, it was concluded that the MS can be
included in complete rations for ruminants at the level of
50-60% for better microbial biomass synthesis which in turn
influences the performance of growing sheep.
Keywords:
complete ration,
in vitro
gas technique, maize stover, roughage to concentrate ratio.
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