Open Access
Research
(Published
online: 30-06-2016)
22.
Molecular characterization of exon 3 of
caprine myostatin gene in Marwari goat -
Jai Prakash Khichar, Gyan Chand Gahlot, Vijay Kumar Agrawal,
Kiran, Ajay Singh Dewna, Prakash and Mohammad Ashraf
Veterinary World, 9(6): 676-679
doi:
10.14202/vetworld.2016.676-679
Jai Prakash Khichar:
Molecular Genetics Laboratory, Department of Animal Genetics and
Breeding, College of Veterinary & Animal Science, Rajasthan
University of Veterinary and Animal Science, Bikaner, Rajasthan,
India; Jai4me007@gmail.com
Gyan Chand Gahlot:
Molecular Genetics Laboratory, Department of Animal Genetics and
Breeding, College of Veterinary & Animal Science, Rajasthan
University of Veterinary and Animal Science, Bikaner, Rajasthan,
India; Gahlotgcbkn@rediffmail.com
Vijay Kumar Agrawal:
Molecular Genetics Laboratory, Department of Animal Genetics and
Breeding, College of Veterinary & Animal Science, Rajasthan
University of Veterinary and Animal Science, Bikaner, Rajasthan,
India; drsonalvet@rediffmail.com
Kiran:
Department of Clinical Veterinary Medicine, Ethics and
Jurisprudence, College of Veterinary and Animal Science, Bikaner,
Rajasthan, India; dr.kiranbeniwal@gmail.com
Ajay Singh Dewna:
Molecular Genetics Laboratory, Department of Animal Genetics and
Breeding, College of Veterinary & Animal Science, Rajasthan
University of Veterinary and Animal Science, Bikaner, Rajasthan,
India; ajaydewna@gmail.com
Prakash:
Molecular Genetics Laboratory, Department of Animal Genetics and
Breeding, College of Veterinary & Animal Science, Rajasthan
University of Veterinary and Animal Science, Bikaner, Rajasthan,
India; drprakashjangu@gmail.com
Mohammad Ashraf
Molecular Genetics Laboratory, Department of Animal Genetics and
Breeding, College of Veterinary & Animal Science, Rajasthan
University of Veterinary and Animal Science, Bikaner, Rajasthan,
India; aicrp@rediffmail.com
Received: 01-02-2016, Accepted: 30-05-2016, Published online:
30-06-2016
Corresponding author:
Jai Prakash Khichar, e-mail: Jai4me007@gmail.com
Citation:
Khichar JP, Gahlot GC, Agrawal VK, Kiran, Dewna AS, Prakash,
Ashraf M (2016) Molecular characterization of exon 3 of caprine
myostatin gene in Marwari goat,
Veterinary World, 9(6):
676-679.
Abstract
Aim:
To estimate genetic variability in exon 3 of caprine myostatin
gene in Marwari goats.
Materials and Methods:
A total of 120 blood samples from unrelated Marwari goats were
randomly collected from different villages of Bikaner
(Rajasthan), India. Genomic DNA was extracted from whole blood
using blood DNA isolation kit (Himedia Ltd.) as per
manufacturer’s protocol. The quality of extracted genomic DNA
was checked on 0.8% agarose gel. Specifically designed a primer
set for caprine myostatin (MSTN)
gene (Genebank accession no. DQ167575) was used to amplify the
exon 3 region of
MSTN
gene in Marwari goat. The genetic variability in exon 3 of
MSTN
gene in Marwari goat was assessed on 8% polyacrylamide gel
electrophoresis to detect single strand conformation
polymorphism (SSCP) pattern.
Results:
The exon 3 of
MSTN
gene in Marwari goat showed two types of conformation patterns
on 8% polyacrylamide gel. One of the patterns showed only two
bands and was considered as genotype AA, whereas another pattern
having an extra band was designated as genotype AB. The
frequencies of AA and AB genotype for exon 3 region of
MSTN
gene were calculated as 0.90 and 0.10, respectively.
Conclusion:
Low level of polymorphism was observed at exon 3 region of
MSTN
gene in Marwari goat through SSCP analysis. This information
could be utilized in future breeding plan to exploit the unique
characteristics of Marwari goat of Rajasthan.
Keywords:
exon 3, Marwari goat, myostatin gene, polymerase chain
reaction-single strand conformation polymorphism.
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