Open Access
Research
(Published
online: 05-06-2016)
4.
Plasma progesterone profile and conception
rate following exogenous supplementation of gonadotropin-releasing
hormone, human chorionic gonadotropin, and progesterone
releasing intra-vaginal device in repeat-breeder crossbred cows
-
N. K. J. Pandey, H. P. Gupta, Shiv Prasad and S. K. Sheetal
Veterinary World, 9(6): 559-562
doi:
10.14202/vetworld.2016.559-562
N. K. J. Pandey:
Department of Veterinary Gynaecology and Obstetrics, College of
Veterinary & Animal Sciences, G.B. Pant University of
Agriculture and Technology, Pantnagar - 263145, Uttarakhand,
India; nitin080132@gmail.com
H. P. Gupta:
Department of Veterinary Gynaecology and Obstetrics, College of
Veterinary & Animal Sciences, G.B. Pant University of
Agriculture and Technology, Pantnagar - 263145, Uttarakhand,
India; hpguptavgo@gmail.com
Shiv Prasad:
Department of Veterinary Gynaecology and Obstetrics, College of
Veterinary & Animal Sciences, G.B. Pant University of
Agriculture and Technology, Pantnagar - 263145, Uttarakhand,
India; shivp2003@yahoo.co.uk
S. K. Sheetal:
Department of Veterinary Gynaecology and Obstetrics, College of
Veterinary & Animal Sciences, G.B. Pant University of
Agriculture and Technology, Pantnagar - 263145, Uttarakhand,
India; sksheetalmuz@gmail.com
Received: 21-12-2015, Accepted: 25-04-2016, Published online:
05-06-2016
Corresponding author:
N. K. J. Pandey, e-mail: nitin080132@gmail.com
Citation:
Pandey NKJ, Gupta HP, Prasad S, Sheetal SK (2016) Plasma
progesterone profile and conception rate following exogenous
supplementation of gonadotropin-releasing hormone, human
chorionic gonadotropin, and progesterone releasing intra-vaginal
device in repeat-breeder crossbred cows,
Veterinary World, 9(6):
559-562.
Abstract
Aim:
This study was designed to evaluate the effect of gonadotropin-releasing
hormone (GnRH), human chorionic gonadotropin (hCG), and
progesterone impregnated intra-vaginal device on progesterone
profile and conception rate in repeat-breeding crossbred cows.
Materials and Methods:
Repeat-breeding crossbred cows aged 3-8 years (n=32), lactating
and negative to white side test were randomly divided into four
groups: Group 1 (Control, n=8), Group 2 (GnRH at 10 μg i.m,
n=8), Group 3 (hCG at 1500 IU i.m., n=8), and Group 4
(progesterone impregnated intra-vaginal device at 958 mg, n=8).
All the treatme nts were given on 5th daypostbreeding
and in Group 4 intra-vaginally implanted device was withdrawn on
9th day (i.e., implant inserted for total 4 days) of
the estrous cycle. Blood samples were collected on day 0, 5, 10,
15, and day 20 of estrous cycle, and plasma was separated for
progesterone estimation.
Results:
Accessory corpus luteum was not formed in crossbred cows of
Group4 and control group. However, total 6 and 8 accessory
corpora lutea were found in Group 2 and Group 3, respectively.
In pregnant cows, the plasma progesterone concentration
increased continuously from day 0 to day 20. In non-pregnant
cows, it increased from day 0 to day 15 and then declined. The
conception rate on day 60 in Group 1, Group 2, Group 3, and
Group 4 was 37.5%, 50%, 75%, and 37.5%, respectively.
Conclusions:
Treating repeat-breeder cows with hCG is effective in increasing
conception rate by developing accessory corpora lutea and higher
progesterone level.
Keywords:
human chorionic gonadotropin, gonadotropin-releasing hormone,
progesterone impregnated device, repeat breeding.
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