Open Access
Research
(Published
online: 10-06-2016)
7.
Diversity of
Meq
gene from clinical Marek’s disease virus
infection in Saudi Arabia -
Mahmoud H. A. Mohamed, Ibrahim M. El-Sabagh, Malik A. Al-Habeeb
and Yousef M. Al-Hammady
Veterinary World, 9(6): 572-578
doi:
10.14202/vetworld.2016.572-578
Mahmoud H. A. Mohamed:
Department of Clinical Studies, College of Veterinary Medicine,
King Faisal University, Al-Hufof, 31982, Saudi Arabia;
Department of Avian and Rabbit Medicine, Faculty of Veterinary
Medicine, Zagazig University, 44519, Zagazig, Egypt;
mahmoudhassanain@yahoo.com
Ibrahim M. El-Sabagh:
Central Biotechnology Laboratory, College of Veterinary
Medicine, King Faisal University, Al-Hufof, 31982, Saudi Arabia;
Department of Virology, Faculty of Veterinary Medicine, Cairo
University, 12211, Giza, Egypt; ibrahimelsabagh@yahoo.com
Malik A. Al-Habeeb:
Excutive Department of Risk Assessment, Saudi Food and Drug
Authority, Saudi Arabia; mxm0063@hotmail.com
Yousef M. Al-Hammady:
Central Veterinary Laboratory, Riyadh, Saudi Arabia; ymh_82_01@hotmail.com
Received: 27-01-2016, Accepted: 22-04-2016, Published online:
10-06-2016
Corresponding author:
Ibrahim M. El-Sabagh, e-mail: ibrahimelsabagh@yahoo.com
Citation:
Mohamed MHA, El-Sabagh IM, Al-Habeeb MA, Al-Hammady YM (2016)
Diversity of
Meq
gene from clinical Marek’s disease virus infection in Saudi
Arabia,
Veterinary World, 9(6):
572-578.
Abstract
Aim:
The aim of this study was to demonstrate the genomic features of
Meq
gene of Marek’s disease virus (MDV) recently circulating in
Saudi Arabia (SA).
Materials and Methods:
Two poultry flocks suffering from mortalities and visceral
tumors were presented to the Veterinary Teaching Hospital, King
Faisal University, SA. Subjected to different diagnostic
procedures: Case history, clinical signs, and necropsy as well
as polymerase chain reaction followed by
Meq
gene sequence analysis.
Results:
Case history, clinical signs, and necropsy were suggestive of
MDV infection. The
Meq
gene was successfully detected in liver and spleen of infected
chickens. A 1062 bp band including the native
Meq
ORF in addition to a 939 bp of
S-Meq
(short isoform of
Meq)
were amplified from Saudi 01-13 and Saudi 02-13, respectively.
The nucleotide and deduced amino acids sequences of the
amplified
Meq
genes of both Saudi isolates showed distinct polymorphism when
compared with the standard USA virulent isolates Md5 and GA. The
sequence analysis of the
S-Meq
gene showed a 123 bp deletion representing 41 amino acids
between two proline-rich areas without any frameshift. The
Meq
gene encoded four repeats of proline-rich repeats (PRRs
sequences), whereas the
S-Meq
contains only two PRRs. Interestingly, the phylogenetic analysis
revealed that both of SA MDV isolates are closely related to the
MDV strains from Poland.
Conclusion:
The two MDV isolates contain several nucleotide polymorphisms
resulting in distinct amino acid substitutions. It is suggested
that migratory and wild birds, as well as world trading of
poultry and its by-products, have a great contribution in the
transmission of MDVs overseas.
Keywords:
deletion, Marek’s disease virus,
Meq
gene sequence, phylogenic analysis, Saudi Arabia.
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