Open Access
Research
(Published
online: 20-03-2016)
13.
Effect of different antioxidant additives
in semen diluent on cryopreservability (−196°C) of buffalo semen
-
Hardik A. Patel, G. M. Siddiquee, Dinesh V. Chaudhari and Vishal
S. Suthar
Veterinary World, 9(3): 299-303
doi:
10.14202/vetworld.2016.299-303
Hardik A. Patel:
Sabarmati Ashram Gaushala, Ahmedabad, Gujarat, India; hapatel@sagbidaj.org
G. M. Siddiquee:
Department of Gynaecology and Obstetrics, College of Veterinary
Sceicne and Animal Husbandry, Sardarkrushinagar Dantiwada
Agricultural University, Deesa, Banaskanth, Gujarat, India; gms@yahoo.co.in
Dinesh V. Chaudhari:
Pashupalan Sansodhan Kendra, Veterinary College, Anand
Agricultural University, Anand, Gujarat, India; dvc@aau.in
Vishal S. Suthar:
Directorate of Research, Kamdhenu University, Gandhinagar,
Gujarat, India; vsuthar28@gmail.com
Received: 04-10-2015, Revised: 30-01-2016, Accepted: 06-02-2016,
Published online: 20-03-2016
Corresponding author:
Vishal S. Suthar, e-mail: vsuthar28@gmail.com
Citation:
Patel HA, Siddiquee GM, Chaudhari DV, Suthar VS (2016) Effect of
different antioxidant additives in semen diluent on
cryopreservability (−196°C) of buffalo semen,
Veterinary World, 9(3):
299-303.
Abstract
Aim:
The aim of this study was to evaluate the effect of different
antioxidant additives in standard tris-fructose-egg yolkglycerol
(TFYG) extender on the cryopreservability of buffalo semen.
Materials and Methods:
Semen collection using artificial vagina, twice weekly for 5
weeks from three pedigreed health breeding bulls of Mehsani
breed, aged between 6 and 8 years. Immediately after initial
evaluation all 30 qualifying ejaculates (10/bull) were split
into three aliquots and diluted at 34°C keeping the
concentration of 100 million spermatozoa/ml with standard TFYG
extender as control and TFYG having two antioxidant additives -
Cysteine HCl at 1 mg/ml and ascorbic acid at 0.2 mg/ml to study
their comparative performance. Semen filled in French Mini
straws using IS-4 system and gradually cooled to 4°C and
equilibrated for 4 h in cold handing cabinet. After completion
of equilibration, straws were cryopreserved in LN2 by
Programmable Bio-freezer. Semen was examined at post-dilution,
post-equilibration, and post-thaw stages for sperm quality
parameters, and at each stage plasma was separated for enzymatic
analysis of aspartate aminotransferase (AST), lactate
dehydrogenase (LDH), and alkaline phosphatase
(AKP).
Results:
The mean percentage of sperms in TFYG, TFYG + cysteine HCl and
TFYG + ascorbic acid diluents at postthaw stage in terms of
progressive motility (52.83±0.52, 57.83±0.52, 57.83±0.52),
livability (78.70±0.21, 82.33±0.23, 81.73±0.22), and abnormality
(5.43±0.21, 5.03±0.17, 5.23±0.18) varied significantly (p<0.05)
between control TFYG and TFYG having antioxidant additives. The
mean U/L activities of AST (78.70±0.47, 72.80±0.48, 73.30±0.54),
LDH (172.70±0.41, 155.78±0.42, 156.33±0.41), and AKP
(103.61±0.34, 90.20±0.34, 91.03±0.34) in semen diluted with TFYG,
TFYG + cysteine HCl and TFYG + ascorbic acid diluents at
post-thaw stage, respectively, which showed significantly
(p<0.05) higher leakage of enzymes in control TFYG than TFYG
incorporated with additives.
Conclusion:
Incorporation of antioxidant additives such as cysteine HCl and
ascorbic acid in standard TFYG diluents improves sperm quality
parameters, reduces enzyme leakage, and ultimately advances
cryopreservability of buffalo semen.
Keywords:
antioxidants, cryopreservation, diluent, egg yolk, enzyme,
semen.
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