Open Access
Research
(Published
online: 25-03-2016)
15.
Evaluation of specific humoral
immune response in pigs vaccinated with cell culture adapted
classical swine fever vaccine -
Mrinal K. Nath,
D. K. Sarma, B. C. Das, P. Deka,
D. Kalita,
J. B. Dutta,
G. Mahato,
S. Sarma
and P. Roychoudhury
Veterinary World, 9(3): 308-312
doi:
10.14202/vetworld.2016.308-312
Mrinal K. Nath :
Department of
Veterinary Epidemiology and Preventive Medicine, College of
Veterinary Science, Assam Agricultural University, Khanapara,
Guwahati, Assam, India; mrinalnath2007@yahoo.com
D. K. Sarma :
ICAR-National
Research Centre on Pig, Rani, Guwahati, Assam, India;
dksarma1956@gmail.com
B. C. Das :
Department of
Veterinary Epidemiology and Preventive Medicine, College of
Veterinary Science, Assam Agricultural University, Khanapara,
Guwahati, Assam, India; bipban.das@gmail.com
P. Deka :
Department of
Veterinary Microbiology, College of Veterinary Science, Assam
Agricultural University, Khanapara, Guwahati, Assam, India;
drpankajaau@gmail.com
D. Kalita :
ICAR - All India
Co-ordinated Research Project on Pigs, College of Veterinary
Science, Assam Agricultural University, Khanapara, Guwahati,
Assam, India; dhireswar64@gmail.com
J. B. Dutta :
Department of
Veterinary Epidemiology and Preventive Medicine, College of
Veterinary Science, Assam Agricultural University, Khanapara,
Guwahati, Assam, India; duttajyotib@gmail.com
G. Mahato :
Department of
Veterinary Epidemiology and Preventive Medicine, College of
Veterinary Science, Assam Agricultural University, Khanapara,
Guwahati, Assam, India; gaurangamahato@yahoo.com
S. Sarma :
Department of
Veterinary Bio-chemistry, College of Veterinary Science, Assam
Agricultural University, Khanapara, Guwahati, Assam, India;
sarmasatya49@yahoo.com
P. Roychoudhury :
Department of
Veterinary Microbiology, College of Veterinary Science and
Animal Husbandry, Central Agricultural University, Aizawal,
Mizoram, India; parimal74@rediffmail.com
Received: 28-12-2015, Revised: 27-01-2016, Accepted: 02-02-2016,
Published online: 25-03-2016
Corresponding author:
Mrinal K. Nath, e-mail: mrinalnath2007@yahoo.com
Citation:
Nath MK, Sarma DK, Das BC, Deka P, Kalita D, Dutta JB, Mahato G,
Sarma S, Roychoudhury P (2016) Evaluation of specific humoral
immune response in pigs vaccinated with cell culture adapted
classical swine fever vaccine, Veterinary World, 9(3):
308-312.
Abstract
Aim:
To determine
an efficient vaccination schedule on the basis of the humoral
immune response of cell culture adapted live classical swine
fever virus (CSFV) vaccinated pigs and maternally derived
antibody (MDA) in piglets of vaccinated sows.
Materials and
Methods:
A cell culture
adapted live CSFV vaccine was subjected to different vaccination
schedule in the present study. Serum samples were collected
before vaccination (day 0) and 7, 14, 28, 42, 56, 180, 194, 208,
270, 284 and 298 days after vaccination and were analyzed by
liquid phase blocking enzyme-linked immunosorbent assay.
Moreover, MDA titre was detected in the serum of piglets at 21
and 42 days of age after farrowing of the vaccinated sows.
Results:
On 28
days after vaccination, serum samples of 83.33% vaccinated pigs
showed the desirable level of antibody titer (log10
1.50 at 1:32 dilution), whereas 100% animals
showed log10
1.50 at 1:32 dilution after 42 days of
vaccination. Animals received a booster dose at 28 and 180 days
post vaccination showed stable high-level antibody titre till
the end of the study period. Further, piglets born from pigs
vaccinated 1 month after conception showed the desirable level
of MDA up to 42 days of age.
Conclusion:
CSF
causes major losses in pig industry. Lapinised vaccines against
CSFV are used routinely in endemic countries. In the present
study, a cell culture adapted live attenuated vaccine has been
evaluated. Based on the level of humoral immune response of
vaccinated pigs and MDA titer in piglets born from immunized
sows, it may be concluded that the more effective vaccination
schedule for prevention of CSF is primary vaccination at 2
months of age followed by booster vaccination at 28 and 180 days
post primary vaccination and at 1 month of gestation.
Keywords:
antibody titer, classical swine fever vaccine, liquid phase
blocking-enzyme-linked immunosorbent assay, pig.
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