Open Access
Research
(Published
online: 26-03-2016)
17.
Ultrastructural study on the granulocytes
of Uttara fowl (Gallus domesticus) -
Khan Idrees Mohd, Meena Mrigesh, Balwinder Singh and Ishwar
Singh
Veterinary World, 9(3): 320-325
doi:
10.14202/vetworld.2016.320-325
Khan Idrees Mohd:
Department of Veterinary Anatomy, College of Veterinary & Animal
Sciences, G.B. Pant University of Agriculture and Technology,
Pantnagar, Uttarakhand, India; idrees413@gmail.com
Meena Mrigesh:
Department of Veterinary Anatomy, College of Veterinary & Animal
Sciences, G.B. Pant University of Agriculture and Technology,
Pantnagar, Uttarakhand, India; meenamrigesh@gmail.com
Balwinder Singh:
Department of Veterinary Anatomy, College of Veterinary & Animal
Sciences, G.B. Pant University of Agriculture and Technology,
Pantnagar, Uttarakhand, India; dhot_balwinder@yahoo.co.in
Ishwar Singh:
Department
of Veterinary Anatomy, College of Veterinary & Animal Sciences,
G.B. Pant University of Agriculture and Technology, Pantnagar,
Uttarakhand, India; singh_iswar@yahoo.co.in
Received: 16-08-2015, Revised: 27-01-2016, Accepted: 09-02-2016,
Published online: 26-03-2016
Corresponding author:
Meena Mrigesh, e-mail: meenamrigesh@gmail.com
Citation:
Mohd KI, Mrigesh M, Singh B, Singh I (2016) Ultrastructural
study on the granulocytes of Uttara fowl (Gallus domesticus),
Veterinary World, 9(3): 320-325.
Abstract
Aim:
The present
study was conducted to know the ultrastructural detail of the
blood cells of Uttara fowl (native fowl of Uttarakhand).
Materials and
Methods:
The experiment
was conducted on 10 apparently healthy adult birds of either sex
reared at the Instructional Poultry Farm, G.B. Pant University
of Agriculture and Technology, Pantnagar, Uttarakhand. The blood
was collected from wing vein using ethylenediamine tetraacetic
acid as anticoagulant. The blood was further processed for
scanning and transmission electron microscopic (TEM) studies
separately.
Results:
Ultrastructurally, the heterophils were irregularly round in
shape. The cytoplasm was laden with pleomorphic membrane-bound
granules, viz., large elliptical-, medium oval-, large
round-, and medium round-shaped granules. The eosinophils under
TEM were irregularly circular in outline showing pseudopodia and
finger-like cytoplasmic processes. The cytoplasmic granules were
pleomorphic with elliptical-, round-, and rod-shaped granules.
The basophils were irregularly circular in outline containing
small hook-like cytoplasmic processes. The cytoplasm contained
electron dense and electron lucent round-shaped granules.
Conclusion:
Granulocytes contained pleomorphic cytoplasmic granules.
However, the shape and electron density of granules varied among
the different granulocytes and helped in the characterization of
different granulocytes.
Keywords:
blood cells, cytoplasmic granules, ultrastructure, Uttara fowl.
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