Open Access
Research
(Published
online: 28-03-2016)
19.
Occurrence of pathogenic
Vibrio parahaemolyticus in crustacean shellfishes in coastal
parts of Eastern India -
S. Parthasarathy, Suresh Chandra Das
and Ashok Kumar
Veterinary World, 9(3): 330-336
doi:
10.14202/vetworld.2016.330-336
S. Parthasarathy :
Division of
Veterinary Public Health, Indian Veterinary Research Institute,
Izatnagar, Bareilly - 243 122, Uttar Pradesh, India;
parthasarathyvet@gmail.com
Suresh Chandra
Das :
Veterinary Public
Health Laboratory, Indian Veterinary Research Institute, Eastern
Regional Station, Kolkata - 700 037, West Bengal, India;
dasivriers@gmail.com
Ashok Kumar :
Assistant Director
General (Animal Health), Indian Council of Agricultural
Research, Krishi Bhawan, New Delhi, India; ashokakt@rediffmail.com
Received: 20-11-2015, Revised: 15-02-2016, Accepted: 22-02-2016,
Published online: 28-03-2016
Corresponding author:
Suresh Chandra Das, e-mail: dasivriers@gmail.com
Citation:
Parthasarathy S, Das SC, Kumar A (2016) Occurrence of pathogenic
Vibrio parahaemolyticus in crustacean shellfishes in
coastal parts of Eastern India, Veterinary World, 9(3):
330-336.
Abstract
Aim:
The
objective of the study was to isolate and characterize
pathogenic Vibrio parahaemolyticus from crustacean
shellfishes (crab and shrimp) commonly retailed in coastal parts
of eastern India.
Materials and
Methods:
Samples were
processed by bacteriological isolation followed by biochemical
characterization in Kaper’s medium. Presumptively identified
isolates were confirmed by species-specific Vp-toxR
polymerase chain reaction (PCR) assay. Virulence and pandemic
property of the confirmed V. parahaemolyticus isolates
were determined by specific PCR assays.
Results:
On
screening of 167 samples comprising crabs (n=82) and shrimps
(n=85) by the standard bacteriological cultural method, V.
parahaemolyticus was presumptively identified in 86.6%
(71/82) and 82.3% (70/85) of respective samples. Of these, 46
(56%) and 66 (77.6%) isolates from crab and shrimp,
respectively, were confirmed as V. parahaemolyticus
by biochemical characterization (Kaper’s reaction) followed
by specific Vp-toxR PCR assay. About 10 isolates each
from crab and shrimp was found to carry the virulence gene (tdh).
It denotes that 12.2% of crab and 11.7% of shrimp in the study
area are harboring the pathogenic V. parahaemolyticus.
Such tdh+
isolates (n=20) were subjected for screening of
pandemic genotype by pandemic group specific (PGS) - PCR (PGS-PCR)
and GS-PCR (toxRS gene) where 11 (6.5%) isolates revealed
the pandemic determining amplicon (235 bp) in PGS-PCR and
belonged to crab (7.3%) and shrimp (6%) samples; however, 2
(2.4%) isolates were positive in GS-PCR and belonged to crab
samples only. These two GS-PCR+
isolates from crab were also positive in PGS-PCR.
Conclusion:
The
findings of the study conclusively indicated that a considerable
percentage of crab and shrimp in these areas were harboring
pathogenic and pandemic V. parahaemolyticus posing a
public health risk in consumption of improperly processed such
shellfishes. Cross contamination of other marine and fresh water
market fishes by such shellfishes in these areas may provide
scope for spreading this pathogen in community food chain.
Keywords:
crustaceans, pandemic, pathogenic, toxR-gene, Vibrio
parahaemolyticus.
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